遗传 ›› 2014, Vol. 36 ›› Issue (12): 1256-1260.doi: 10.3724/SP.J.1005.2014.1256

• 研究报告 • 上一篇    下一篇

陆地棉皱缩叶突变体基因wr3的初步定位

李峰利1, 狄佳春2, 赵亮2, 陈旭升2   

  1. 1. 南京农业大学农学院, 南京 210095;
    2. 江苏省农业科学院, 农业部长江下游棉花与油菜重点实验室, 南京 210014
  • 收稿日期:2014-06-04 出版日期:2014-12-20 发布日期:2014-12-20
  • 通讯作者: 陈旭升, 研究员, 研究方向:棉花遗传育种。E-nail: njcxs@126.com E-mail:776840727@qq.com
  • 作者简介:李峰利, 硕士研究生, 研究方向:棉花分子遗传育种。
  • 基金资助:

    转基因生物新品种培育科技重大专项子课题(编号:2011ZX08005-001)和转基因特色专用棉新品种培育项目(编号:2011ZX08005-005)资助

Mapping of a new wrinkled leaf (wr3) gene in upland cotton

Fengli Li1, Jiachun Di2, Liang Zhao2, Xusheng Chen2   

  1. 1. Agronomy College of Nanjing Agricultural University, Nanjing 210095, China;
    2. Key Laboratory of Cotton and Rape in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2014-06-04 Online:2014-12-20 Published:2014-12-20

摘要:

陆地棉皱缩叶突变体是本项目组自主发现的一种自然突变体。前期对其表型性状的观察发现该突变体的农艺性状有别于前人已报道的皱叶突变体;通过陆陆杂交世代群体对其遗传规律的研究表明, 皱缩叶突变性状是受一对完全隐性基因wr3控制的质量性状。文章以皱叶突变自交系L037和海7124为亲本配置组合得到的海陆杂交F2为作图群体, 利用本实验室遴选的平均覆盖棉花26对染色体上的234对SSR引物(每染色体相对等距离分布9对引物), 通过对双亲以及近等基因池的筛选, 共得到12对多态性引物。用这些引物检测F2作图群体每个单株的标记基因型, 经Join Map 4.0分析显示, 位于Chr.21的引物BNL3279与目的基因连锁, 二者间的遗传距离为28.7 cM。随后对Chr.21上的其他引物进一步筛选, 共得到16对与目的基因连锁的标记, 其中与目的基因距离最近的标记NAU3740的遗传距离为4.8 cM, 另一侧标记cgr5428的遗传距离为10.4 cM。由此推定, 皱缩叶突变体基因wr3在棉花第21染色体上。

关键词: 陆地棉, 皱缩叶突变体, 基因定位, SSR, BSA分析法

Abstract:

The phenotype of a wrinkled leaf mutant in upland cotton newly found was different from other analogous mutants reported previously. Genetic analysis indicates that the mutation is controlled by a single recessive gene wr3. In this paper, an F2 mapping population derived from the mutation inbred line L037 crossing with Hai7124 (Gossypium babardense) was used. A total of 12 pairs of polymorphic primers were selected from 234 pairs of SSR primers obtained before, which covered 26 pairs of cotton chromosomes at regular intervals. Genetic mapping of the F2 population showed that gene wr3 was linked to the primer BNL3279 on Chr.21 with the distance of 28.7 cM. Further screening of other primers on Chr.21 showed that gene wr3 was flanked by the primers NAU3740 and cgr5428, with genetic distance of 4.8 cM and 10.4 cM, respectively.

Key words: Gossypium hirsutum, wrinkled leaf mutant, gene mapping, SSR, bulked segregation analysis