核糖体蛋白RPL15 cDNA序列在真骨鱼类系统进化研究中的价值 &

遗传 ›› 2006, Vol. 28 ›› Issue (2): 171-178.

核糖体蛋白RPL15 cDNA序列在真骨鱼类系统进化研究中的价值
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向筑¹; 张竞男¹; 宋平¹; 胡珈瑞²; 钟扬³

1. 武汉大学生命科学学院教育部发育生物学重点实验室, 武汉430072; 2. 武汉大学中南医院, 武汉430071; 3. 复旦大学生命科学学院生物多样性与生态工程教育部重点实验室, 上海200433

收稿日期:2004-11-26 修回日期:2005-07-24 出版日期:2006-02-10 发布日期:2006-02-10
通讯作者: 宋平

The Study of Ribosomal Protein L15 cDNA Sequences as a Molecular Marker in the Teleostei Phylogenetic Analysis

XIANG Zhu¹, ZHANG Jin-Nan¹, SONG Ping¹, HU Jia-Rui², ZHONG Yang³

1. Key Laboratory of the Ministry of Education for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072,China; 2. Zhongnan Hospital, Wuhan University, Wuhan 430071, China; 3. Ministry of Education, Key Laboratory for Biodiversity Science and Ecological Engineering, Shanghai 200433 China

Received:2004-11-26 Revised:2005-07-24 Online:2006-02-10 Published:2006-02-10
Contact: SONG Ping

摘要/Abstract

摘要：

应用RT-PCR, 从真骨总目（Teleostei）5目15种鱼类中首次克隆了核糖体大亚基蛋白L15 (RPL15, ribosomal protein L15) 的完整cDNA序列。以海鲢形亚组（Elopomorpha）的鳗鲡作为外类群, 对这些真骨鱼类的核糖体蛋白L15 cDNA序列进行了系统发育分析, 结果表明: (1)RPL15基因在真骨鱼类等许多真核生物进化中高度保守; (2)系统树中各物种之间的关系与形态分类一致。RPL15编码区适合于真骨鱼类目以上分类阶元的分子系统学研究。

关键词: 分子系统学, 核糖体蛋白L15（RPL15）, 真骨鱼类

Abstract: In this study, the full-length cDNAs of RPL15 (ribosomal protein L15) gene were cloned and sequenced from fifteen fishes of five orders under Teleostei. The complete ORF sequences were analyzed for phylogenetic reconstruction for the first time to evaluate the potential of RPL15 gene as a novel marker in resolving teleostean phylogenetic relationships. The resultant NJ, MP and ML trees with Anguilla japonica as the outgroup were generally resolved and largely congruent with the morphology-based tree though some incongruities were observed. The incongruities were then analyzed for some explanations. The results suggested that: (1) RPL15 gene was highly conserved during eukaryotic evolution; (2) RPL15 ORF might be a good phylogenetic marker for resolving teleostean relationships. It might be especially appropriate for the higher-level relationships (such as interordinal), and it was possibly suitable for lower-level relationships as well. The same can be true for other eukaryotes.

中图分类号:

Q591.2

向筑，张竞男，宋平，胡珈瑞，钟扬. 核糖体蛋白RPL15 cDNA序列在真骨鱼类系统进化研究中的价值

& [J]. 遗传, 2006, 28(2): 171-178.

XIANG Zhu, ZHANG Jin-Nan, SONG Ping, HU Jia-Rui, ZHONG Yang. The Study of Ribosomal Protein L15 cDNA Sequences as a Molecular Marker in the Teleostei Phylogenetic Analysis[J]. HEREDITAS, 2006, 28(2): 171-178.