家蚕性连锁平衡致死系致死基因的SSR定位

doi:10.3724/SP.J.1005.2010.01269

遗传 ›› 2010, Vol. 32 ›› Issue (12): 1269-1274.doi: 10.3724/SP.J.1005.2010.01269

家蚕性连锁平衡致死系致死基因的SSR定位

轩楠, 牛宝龙, 王海龙, 庄俐, 孟智启

浙江省农业科学院蚕桑研究所, 杭州 310021

收稿日期:2010-02-28 修回日期:2010-05-23 出版日期:2010-12-20 发布日期:2010-12-20
通讯作者: 孟智启 E-mail:mengzq2011@sina.com
基金资助:
国家863计划项目(编号：2006AA10A119)、国家863专题项目(编号：2008AA10Z139)和浙江省重大科技专项重点农业项目 (编号：2010C12005)资助

Mapping of the lethal genes in the sex-linkaged balanced lethal silk-worm Bombyx mori using SSR markers

XUAN Nan, NIU Bao-Long, WANG Hai-Long, ZHUANG Li, MENG Zhi-Qi

Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2010-02-28 Revised:2010-05-23 Online:2010-12-20 Published:2010-12-20
Contact: MENG Zhi-Qi E-mail:mengzq2011@sina.com

摘要/Abstract

摘要： 家蚕性连锁平衡致死系(S-14)雄蚕的两条Z染色体分别携带有一个非等位、紧密连锁的隐性胚胎期致死基因l₁(lethal gene 1)和l₂(lethal gene 2)。两个致死基因的致死时期分别是转青期和G₂期。将S-14品系的雄蚕和家蚕P50品系的野生型雌蚕杂交, F₁代雄蚕和P50品系雌蚕回交, 即P50×(P50×S14)。回交后代雌蛾根据父本(F₁代雄蚕)携带l₁ 或l₂ 基因分成两类BC1-l₁ 和BC1-l₂, 分别用来做l₁ 和l₂ 基因定位。利用公布的家蚕全基因组序列筛选l₁ 基因和l₂ 基因所在Z染色体与P50品系Z染色体间的差异SSR标记, 分别获得16个和18个差异性SSR标记, 用差异性标记检测BC1-l₁ 和BC1-l₁, 最终将l₁ 基因定位在Z染色体物理图谱中的19.79 Mb位点到染色体末端约2.60 Mb范围内, 将l₂ 基因定位在Z染色体物理图谱的17.86 Mb位点到18.55 Mb位点约 0.69 Mb范围内。

关键词: 家蚕, 性连锁平衡致死系, 致死基因, SSR, 基因定位

Abstract: The males of sex-linkaged balanced lethal silkworm strain (S-14) has two non-allelic recessive genes (lethal gene 1, l₁ and lethal gene 2, l₂). The two genes are located on two different Z chromosomes and cause death of embryos at body pigmentation stage and end reversal embryo stage, respectively. We firstly hybridized the males of S-14 strain with the females having wild-type genes of P50 strain and then backcrossed the males of F₁ with females of P50 strain. A total of 1660 female moths of BC1 generation were divided into two groups, 1 100 in BC1-l₁ and 560 in BC1-l₁ according to the lethal gene carried by these female moths′ fathers-fame moths of F1₁, respectively. Based on the nucleotide sequence infor-mation from the published physical map of Bombyx mori, we developed 16 polymorphic SSR markers in l₁ gene region and 18 polymorphic SSR makers in l₂ gene region compared to the allelic region of P50 strain and used these SSR markers and groups of BC1-l₁ and BC1-l₂ to map the two lethal genes, respectively. Gene l₁ was mapped on the region of Z chromosome, covering a physical distance of 2.60 Mb. Gene l₂ was fine mapped on the region of Z chromosome, covering a physical distance of 0.69 Mb.

Key words: gene location, Bombyx mrori, sex-linkaged balanced lethal strain, lethal gene, SSR

轩楠，牛宝龙，王海龙，庄俐，孟智启. 家蚕性连锁平衡致死系致死基因的SSR定位[J]. 遗传, 2010, 32(12): 1269-1274.

HAN Nan, NIU Bao-Long, WANG Hai-Long, PENG Li, MENG Zhi-Qi. Mapping of the lethal genes in the sex-linkaged balanced lethal silk-worm Bombyx mori using SSR markers[J]. HEREDITAS, 2010, 32(12): 1269-1274.

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家蚕性连锁平衡致死系致死基因的SSR定位

Mapping of the lethal genes in the sex-linkaged balanced lethal silk-worm Bombyx mori using SSR markers

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