遗传 ›› 2010, Vol. 32 ›› Issue (12): 1269-1274.doi: 10.3724/SP.J.1005.2010.01269

• 研究报告 • 上一篇    下一篇

家蚕性连锁平衡致死系致死基因的SSR定位

轩楠, 牛宝龙, 王海龙, 庄俐, 孟智启   

  1. 浙江省农业科学院蚕桑研究所, 杭州 310021
  • 收稿日期:2010-02-28 修回日期:2010-05-23 出版日期:2010-12-20 发布日期:2010-12-20
  • 通讯作者: 孟智启 E-mail:mengzq2011@sina.com
  • 基金资助:

    国家863计划项目(编号:2006AA10A119)、国家863专题项目(编号:2008AA10Z139)和浙江省重大科技专项重点农业项目 (编号:2010C12005)资助

Mapping of the lethal genes in the sex-linkaged balanced lethal silk-worm Bombyx mori using SSR markers

XUAN Nan, NIU Bao-Long, WANG Hai-Long, ZHUANG Li, MENG Zhi-Qi   

  1. Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2010-02-28 Revised:2010-05-23 Online:2010-12-20 Published:2010-12-20
  • Contact: MENG Zhi-Qi E-mail:mengzq2011@sina.com

摘要: 家蚕性连锁平衡致死系(S-14)雄蚕的两条Z染色体分别携带有一个非等位、紧密连锁的隐性胚胎期致死基因l1(lethal gene 1)和l2(lethal gene 2)。两个致死基因的致死时期分别是转青期和G2期。将S-14品系的雄蚕和家蚕P50品系的野生型雌蚕杂交, F1代雄蚕和P50品系雌蚕回交, 即P50×(P50×S14)。回交后代雌蛾根据父本(F1代雄蚕)携带l1l2 基因分成两类BC1-l1 和BC1-l2, 分别用来做l1l2 基因定位。利用公布的家蚕全基因组序列筛选l1 基因和l2 基因所在Z染色体与P50品系Z染色体间的差异SSR标记, 分别获得16个和18个差异性SSR标记, 用差异性标记检测BC1-l1 和BC1-l1, 最终将l1 基因定位在Z染色体物理图谱中的19.79 Mb位点到染色体末端约2.60 Mb范围内, 将l2 基因定位在Z染色体物理图谱的17.86 Mb位点到18.55 Mb位点约 0.69 Mb范围内。

关键词: 家蚕, 性连锁平衡致死系, 致死基因, SSR, 基因定位

Abstract: The males of sex-linkaged balanced lethal silkworm strain (S-14) has two non-allelic recessive genes (lethal gene 1, l1 and lethal gene 2, l2). The two genes are located on two different Z chromosomes and cause death of embryos at body pigmentation stage and end reversal embryo stage, respectively. We firstly hybridized the males of S-14 strain with the females having wild-type genes of P50 strain and then backcrossed the males of F1 with females of P50 strain. A total of 1660 female moths of BC1 generation were divided into two groups, 1 100 in BC1-l1 and 560 in BC1-l1 according to the lethal gene carried by these female moths′ fathers-fame moths of F11, respectively. Based on the nucleotide sequence infor-mation from the published physical map of Bombyx mori, we developed 16 polymorphic SSR markers in l1 gene region and 18 polymorphic SSR makers in l2 gene region compared to the allelic region of P50 strain and used these SSR markers and groups of BC1-l1 and BC1-l2 to map the two lethal genes, respectively. Gene l1 was mapped on the region of Z chromosome, covering a physical distance of 2.60 Mb. Gene l2 was fine mapped on the region of Z chromosome, covering a physical distance of 0.69 Mb.

Key words: gene location, Bombyx mrori, sex-linkaged balanced lethal strain, lethal gene, SSR