粗糙脉孢菌基因组分泌蛋白的初步分析

遗传 ›› 2006, Vol. 28 ›› Issue (2): 200-207.

粗糙脉孢菌基因组分泌蛋白的初步分析

周晓罡¹;李成云²;赵之伟⁴;苏源⁴;张绍松¹;李进斌³

杨静²;刘林²;业艳芬²

(1. 云南省农业科学院生物技术与种质资源研究所, 昆明650223；2. 云南省农业大学农业生物多样性与病虫害控制教育部重点实验室，昆明 650201； 3. 云南省农业科学院农业环境资源研究所，昆明 650205；4. 云南大学省部共建生物资源保护与利用国家重点实验室培育基地，昆明 650091)

收稿日期:2005-02-16 修回日期:2005-10-08 出版日期:2006-02-10 发布日期:2006-02-10
通讯作者: 李成云

Analysis of the Secreted Proteins Encoded by Genes in Genome of Filamental Fungus ( Neurospora crassa)

ZHOU Xiao-Gang¹ ，LI Cheng-Yun²， ZHAO Zhi-Wei⁴，

SU Yuan⁴，ZHANG Shao-Song¹，LI Jin-Bin³ ，Yang Jing²，LI

(1. Biotechnology&Genetic Germplasm Institute，Yunnan Academy of Agricultural Sciences，Kunming 650223， China; 2. The Ministry of Education Key Laboratory for Agricultural Biodiversity and Pest Management，Yunnan Agricultural University， Kunming 650201，China ; 3. Agriculture Environment & Resource Research Institute，Yunnan Academy of Agricultural Sciences，Kunming 650205， China; 4. Key Laboratory for Conservation and Utilization of Bio-resources，Yunnan University，Kunming 650091， China)

Received:2005-02-16 Revised:2005-10-08 Online:2006-02-10 Published:2006-02-10
Contact: LI Cheng-Yun

摘要/Abstract

摘要：

文章报道利用信号肽预测软件SignalP v3.0和PSORT，跨膜螺旋结构预测软件TMHMMv2.0和THUMBUP，GPI-锚定位点预测软件big-PI Predictor和亚细胞器中蛋白定位分布预测软件TargetP v1.01对粗糙脉孢菌全基因组数据库中已公布的10 082个氨基酸序列进行预测分析。结果表明在粗糙脉孢菌中有437个蛋白为分泌蛋白，编码这些蛋白最小的可读框（open reading frame，ORF）为252 bp，最大为6 604 bp，平均1 433 bp，分泌蛋白信号肽长度介于15~59个氨基酸之间。在437个分泌蛋白中，205个具有功能描述，主要包括各种酶类、细胞能量生成、运转以及自身修复、防卫等多种功能。这些蛋白所参与的生化过程可能发生在膜外的周质空间或是菌体外的场所，为该物种营养的摄取，以及对环境做出响应服务。

关键词: 粗糙脉孢菌, 信号肽, 分泌蛋白

Abstract: The completed genome sequence of filamental fungus Neurospora crassa has completed and 10 082 open reading frames (ORFs) were predicted in whole genome. Among them, 437 proteins were secreted ones based on the combination of prediction algorithms SignalP v3.0 and PSORT, transmembrane domains prediction algorithms TMHMM v2.0 and THUMBUP , potential GPI-anchor sites prediction algorithm big-PI predictor and the subcellular protein location prediction algorithm TargetP v1.01. The minimum ORF length of the encoding sequences for 437 secreted proteins was 252 bp, the maximum ORF length was 6 604 bp and average was 1 433 bp. The length of signal peptide of secreted proteins ranged from 15 to 59 amino acids. Among the 437 secreted proteins, 205 were provided with function description. The function of secretory proteins were involved in kinds of enzyme, cell energy, transmition, cell recovered itself and defense mechanism et al. These results implied that processes catalyzed by these proteins probably occurred outside of cells, at least partially.

中图分类号:

Q 936

周晓罡，李成云，赵之伟，苏源，张绍松，李进斌，杨静，刘林，业艳芬. 粗糙脉孢菌基因组分泌蛋白的初步分析[J]. 遗传, 2006, 28(2): 200-207.

ZHOU Xiao-Gang, LI Cheng-Yun, ZHAO Zhi-Wei, SU Yuan, ZHANG Shao-Song, LI Jin-Bin, Yang Jing, LIU Lin. Analysis of the Secreted Proteins Encoded by Genes in Genome of Filamental Fungus ( Neurospora crassa)[J]. HEREDITAS, 2006, 28(2): 200-207.

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