关键词: 基因结构, 系统进化树, 线粒体基因组, 藏鸡

Abstract: Using PCR amplification, sequencing and assembling, we obtained the complete mitochondrial genome of tibetan chicken. The complete mitochondrial genome was 16783bp in length. It contained 37 genes (13 protein coding genes, 2 rRNA, 22 tRNA) and a control region. The simulated restriction enzymes map revealed the pattern of DraI restriction endonucleases was specialized in tibetan chicken. Phylogenetic trees based on the D-loop locus and the 13 protein coding genes by Neighbor-joining analysis indicated that the red junglefowl was the direct ancestor of tibetan chicken and tibetan chicken was closest to white leghorn and white p1ymouth rock. However, the evolution of Tibetan chicken appeared to be relatively independent from that of white leghorn and white p1ymouth rock. The possible reasons were that the ancestor of Tibetan chicken lived in a relatively isolated environment after entered into high altitude and developed individual genetic characters.