遗传 ›› 2008, Vol. 30 ›› Issue (10): 1241-1248.doi: 10.3724/SP.J.1005.2008.01241

• 综述 • 上一篇    下一篇

蛙科两栖动物皮肤抗菌肽的分子多样性及功能

金莉莉; 王秋雨

  

  1. 辽宁大学生命科学院, 沈阳 110036

  • 收稿日期:2008-02-22 修回日期:2008-05-14 出版日期:2008-10-10 发布日期:2008-10-10
  • 通讯作者: 王秋雨

Molecular diversities and functions of antibacterial peptides from the skins of Ranidae of amphibians

JIN Li-Li; WANG Qiu-Yu   

  1. School of Life Science, Liaoning University, Shenyang 110036, China
  • Received:2008-02-22 Revised:2008-05-14 Online:2008-10-10 Published:2008-10-10
  • Contact: WANG Qiu-Yu

摘要:

摘要: 蛙科(Ranidae)是全球分布最广泛的两栖动物, 种类超过650种。为开拓和适应广阔的栖息地及多样的生态环境, 其皮肤腺体中进化产生了结构复杂、种类繁多的抗菌肽。它们除具有广谱抗菌活性外, 还有抗肿瘤、抗病毒等生物学活性。蛙科动物皮肤抗菌肽起源于共同祖先, 在漫长的进化过程中, 基因发生了多重复制和突变, 形成了天然抗菌肽的巨大资源库。这些肽在模拟膜的溶剂中几乎都是疏水的, 并带正电荷, 以一种两亲性的α-螺旋的构象存在。根据氨基酸组成及结构的相似性, 可以将蛙科动物抗菌肽分成brevinin-1、esculentin-1、esculentin-2、temporin、ranalexin、ranatuerin-1、ranatuerin-2、plaustrin、brevinin-2、tigerinin、japonicin、nigrocin和melittin相关肽等若干个家族。文章结合作者的研究工作, 综述了目前已经鉴定的蛙科动物皮肤抗菌肽的分子多样性特点、家族性质和生物学活性的研究进展, 并阐述了东北林蛙新家族抗菌肽的特点。
关键词: 蛙科; 抗菌肽家族; 分子多样性; 生物学活性

关键词: 分子多样性, 生物学活性, 蛙科, 抗菌肽家族

Abstract:

Abstract: Granular glands in the frog skins of Ranidae of amphibians, a widely distributed group with over 650 spe-cies, synthesize and secrete a remarkably diverse array of peptides with the broad-spectrum antibacterial, antifungal and other biologic activities to protect the organism against a wide range of pathogens, which are believed to have arisen as a result of multiple gene duplication events. Almost without exception, these components are hydrophobic, cationic and form an amphipathic α-helix in a membrane-mimetic solvent. The peptides can be grouped into families on the basis of structural similarity. To date, brevinin-1, esculentin-1, esculentin-2, and temporin peptides, ranalexin, ranatuerin-1, ranatuerin-2 and palustrin, brevinin-2, tigerinin, japonicin, nigrocin and melittin-related peptides have been found in amphibians of Ranidae. In this paper, the molecular diversity, structural feature and the biological ac-tivity of Ranidae antibacterial peptides were reviewed.