DNA技术在海洋食品物种鉴定中的应用

doi:10.3724/SP.J.1005.2010.00555

遗传 ›› 2010, Vol. 32 ›› Issue (6): 555-560.doi: 10.3724/SP.J.1005.2010.00555

DNA技术在海洋食品物种鉴定中的应用

张丽¹, 张良², 刘书成², 张义军³, 韩艺⁴

1. 广东海洋大学农学院, 湛江524025;
2. 广东海洋大学食品科技学院, 湛江524025;
3. 河南省内黄县安乡农业服务中心, 安阳 456300;
4. 河南省鹿邑县畜牧局, 周口 477200

收稿日期:2009-09-27 修回日期:2010-01-03 出版日期:2010-06-20 发布日期:2010-05-24
通讯作者: 刘书成 E-mail:Lsc771017@163.com
基金资助:
广东海洋大学博士启动基金项目(编号：0712130)资助

DNA-based methods for identification of seafood species

ZHANG Li¹, ZHANG Liang², LIU Shu-Cheng², ZHANG Yi-Jun³, HAN Yi⁴

1. Agricultural College, Guangdong Ocean University, Zhanjiang 524025, China;
2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524025, China;
3. Anxiang Agricultural Service Center, Neihuang County, Anyang 456300, China;
4. Luyi Farming Bureau, Henan Province, Zhoukou 477200, China

Received:2009-09-27 Revised:2010-01-03 Online:2010-06-20 Published:2010-05-24
Contact: LIU Shu-Cheng E-mail:Lsc771017@163.com

摘要/Abstract

摘要：

随着分子生物学技术的发展, 海洋食品物种鉴定方法由原来的蛋白质水平深入到了DNA水平。目前应用于海洋食品物种鉴定的DNA技术主要是FINS(Forensically informative nucleotide sequencing)、PCR-RFLP和物种特异性PCR标记技术等, 能够实现对新鲜、冰冻、腌制或灌装食品物种进行鉴定, 而对混合样本的鉴定及量化分析是尚待解决的一个问题。基因数据库对物种鉴定的影响也越来越大, 是海洋加工食品物种鉴定可利用的另一种重要信息资源。文章综述了DNA技术在海洋食品物种鉴定中的应用研究进展, 并展望DNA技术在海洋食品检测中的发展趋势。

关键词: DNA技术, 海洋食品, 物种鉴定

Abstract:

With the development of molecular biotechnology, methods for identification of seafood species are developed from protein to DNA. At present, the main DNA-based methods for species identification are FINS, PCR-RFLP, and specific-PCR, which have been used to identify the species of fresh, frozen, and pickled or canned seafood. However, qualitative and quantitative methods for identification of the mixed seafood species remain to be resolved. The gene databases play an important role in identifying species and are valuable information resources for identification of seafood species. In this paper, recent progresses of major DNA-based methods for identification of seafood species are reviewed and the perspec-tives of this field are discussed.

Key words: DNA biotechnology, seafood, species identification

张丽，张良，刘书成，张义军，韩艺. DNA技术在海洋食品物种鉴定中的应用[J]. 遗传, 2010, 32(6): 555-560.

ZHANG Li, ZHANG Liang, LIU Shu-Cheng, ZHANG Xi-Jun, HAN Yi. DNA-based methods for identification of seafood species[J]. HEREDITAS, 2010, 32(6): 555-560.

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DNA技术在海洋食品物种鉴定中的应用

DNA-based methods for identification of seafood species

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