遗传 ›› 1993, Vol. 15 ›› Issue (3): 16-19.

• 论文 • 上一篇    下一篇

微核直径测试作为非整倍体诱发剂的分析手段

汪旭;李雯;周汝敏;曹能;刘素清 WANG Xu;LI Wen;ZHOU Ru-Min;CAO Neng;LIU Su-Qing   

  1. 云南师范大学生物系,昆明 650092 Department of Biology,Yunnan Normal University,Kunming 650092
  • 收稿日期:1900-01-01 出版日期:1993-06-10 发布日期:1993-06-10

Measurement of Micronucleus Diameters as a Test for the Detection of Aneugens

  • Received:1900-01-01 Online:1993-06-10 Published:1993-06-10

摘要: 以小鼠骨髓红细胞微核直径测试,比较了秋水仙素(COL)与昆明山海棠(THH)、对苯二酚(HQ)在哺乳动物体细胞中的非整倍体诱发效应。丝裂霉素C(MMC)作为多功能染色体断裂剂引入实验,为诱发非整倍体的阴性对照。结果发现,COL组,71%的微核直径(d)大于或等于所在细胞直径(D)的五分之一(d≥D/5);THH诱发微核中,54%的微核d≥D/5;HQ及MMC组,分别有47%及14%的微核相对直径达此阈值。暗示THH及HQ具有类似COL的某种非整倍体诱发效应。微核直径测试可作为非整倍体诱发剂检测的辅助手段。
The relative diameters of micronucleus induced by colchicines(COL),Triptergium hypoglaucum(Level)Hutch(THH)and hydroquinone(HQ)were compared to evaluate their aneugenic activities in mouse bone marrow erythrocytes.MitomycinC(MMC)was taken as the negative control in the experiment because it is a multifunctional clastogen without aneugenic potential.Diameters of the cytoplasm(D)and the micronucleus(d)of each micronucleated erythrocytes were measured with a micrometer in a microscope.The frequency of relatively large micronuclei(d≥D/5)was found(71%) in COL treated group.In the THH and HQ treated groups,the relatively large micronuclei were 54% and 47%,respectively.Such micronuclei were infrequent(14%)in the MMC treated group.The results implied that THH and HQ may possess some aneugenic potential like COL.

关键词: 非整倍体, 纺锤体毒剂, 有丝分裂抑制剂, 微核直径测试, 小鼠骨髓红细胞
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