遗传 ›› 1997, Vol. 19 ›› Issue (5): 37-40.

• 论文 • 上一篇    下一篇

具有高外切葡聚糖纤维二糖水解酶Ⅰ活力的新型黄单胞工程菌的构建

王春卉; 汪天虹; 高培基; 钟玲 WANG Chun-Hui;WANG Tain-Hong;GAO Pei-Ji;ZHONG Ling   

  1. 山东大学生命科学学院微生物系,济南 250100 Microbiology Department,The college of Life Science,Shandong University,Jinan 250100
  • 收稿日期:1900-01-01 出版日期:1997-10-10 发布日期:1997-10-10

Construction of Novel Engineering Xanthomonas with Higher Cellubiohydrolase I Activity

  • Received:1900-01-01 Online:1997-10-10 Published:1997-10-10

摘要: 由广宿主质粒pRK404和微紫青霉外切葡聚糖纤维二糖水解酶Ⅰ(CBHI)c DNA基因cbhⅠ构建了重组质粒pRK404-181,在携带有诱动质粒的菌株ED86 54(pRK2013)的存在下, 采用三亲滤膜结合的方法,将该质粒转移到野油菜黄单胞菌S-152中,并成功地得到表达。结合子S-152(pRK404-181)外切葡聚糖纤维二糖水解酶的pNPC活力比原始菌株S-152提高了2-4倍,滤纸酶活提高了1倍。
Abstract:Recombinant plasmid pRK404-181 was constructed with the wide-host-range plasmid pRK404 and the cellubiohydrolase I(cbh I)gene of Penicillium janthinellum.The plasmid was transferred into Xanthomonas campestris S-152 with the aid of helper plasmid pRK2013.As a result,the intracellular collubiohydrolase activity of S-152(pPK404-181)increased 2~4 time,and the filter paper degrading activity increased 1 time in comparison with the initial bacterial Xanthomonas campestris S-152.

关键词: 接合转移, 微紫青霉, Conjugation, 外切葡聚糖纤维二糖水解酶基因
Key words,
野油菜黄单胞菌, 亚型, 马铃薯, classⅠpatatin基因, 块茎

Key words: Subtype, Tuber, class I patatin gene, Potato