遗传 ›› 2001, Vol. 23 ›› Issue (3): 157-158.

• 论文 •    下一篇

中国鄂温克、鄂伦春、达斡尔族永生细胞系的建立与保存

薛雅丽;王琦;史忠诚;刘岸;张钰;黄小义;黄承滨;陈白滨;杨焕杰;傅松滨;李璞 XUE Ya-li;WANG Qi;SHI Zhong-cheng;LIU An;ZHANG Yu;HUANG Xiao-yi; HUANG Cheng-bin;CHEN Bai-bin;YANG Huan-jie;FU Song-bin;LI Pu   

  1. 哈尔滨医科大学医学遗传学研究室,黑龙江 哈尔滨 150086 Laboratory of Medical Genetics,Department of Medical Biology,Harbin Medical University,Harbin 150086,China
  • 收稿日期:1900-01-01 出版日期:2001-06-10 发布日期:2001-06-10

Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the Ewenki,the Oroqen and the Daur Ethnic Groups in China

  • Received:1900-01-01 Online:2001-06-10 Published:2001-06-10

摘要: 本文采用EBV(Epstein?Barr Virus)上清液转化B淋巴细胞,并加入环胞霉素A(Cyclosporine A)抑制T淋巴细胞,成功地对中国东北地区鄂温克族、鄂伦春族及达斡尔族的部分个体建立了永生细胞系,其中鄂温克族49株,鄂伦春族40株,达斡尔族51株,总计140株。永久保存我国特有民族的基因组,为分析其遗传学差异奠定了基础。
Abstract:The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhabit the activity of T cells.In the present study ,140 immortal cell lines of the Ewenki,the Oroqen and the Daur ethnic groups in the Northeast China were established .This is an important part of the research of human genome diversity for the exploration of the origin and progression of different ethnic groups ,and also provide enough research materials for further studies.

关键词: 少数民族
Key words,
EBV, immortal lymphoblastoid cell line, EBV, 遗传永生细胞系