遗传 ›› 2001, Vol. 23 ›› Issue (5): 445-633.

• 论文 • 上一篇    下一篇

青海湖裸鲤mtDNA遗传多样性的初步研究

赵凯1;李军祥1;张亚平2;罗静2;李太平1;吴华1;田海宁1 ZHAO Kal1;LI Jun-xiang1;ZHANG Ya-ping2;LUO Jing2;LI Tai-ping1;WU Hua1;TIAN Hai-ning1   

  1. 1.青海大学农牧学院动物科学系,西宁 810003 2.中国科学院昆明动物所细胞与分子进化研究开放实验室,昆明 650223 1.College of Agriculture and Animal Husbandry,Qinghai University,Qinghai,Xining 810003,China; 2.Laboratory of Cellular and Molecular Euolution,Kunming Institute of Zoologythe Chinese Academy of Sciences,Yunnan,Kunming 650223,China
  • 收稿日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

Mitochondrial DNA Diversity in Naked Carps in Qinghai-lake (Gymnocypris prezwalskii prezwalskii)

  • Received:1900-01-01 Online:2001-10-10 Published:2001-10-10

摘要: 本文用BclⅠ、AvaⅠ、BamHⅠ、PstⅠ、KpnⅠ、PvuⅡ共6种限制性内切核酸酶,分析了15尾青海湖裸鲤mtDNA的限制性片段长度多态性,共检测出20个酶切位点,发现BclⅠ、BamHⅠ和PvuⅡ三种酶切类型具有多态性.根据不同个体mtDNA的酶切类型,青海湖裸鲤存在4种mtDNA单倍型,计算mtDNA多态度π值为0.0043,初步认为青海湖裸鲤在线粒体DNA上存在较丰富的群体内变异、
Abstract:An analysis of patterns of cleavage of mtDNA by restriction endonucleases was performed for 15 Gymnocypris przewalskii przewalskii.Mitochondrial DNA polymorphisms were detected in the restriction patterns generated by the following 5 enzymes,BclI,AvaI,BamH],PstI,KpnI,PvuII.Only 3 of them (BclI,BantHI,PvuII) were found to be polymorphisms.Our results shorted that there were 4 mtDNA haplotypes in Gyrnnocypris przewalskii przewalskii and the genetic divergenec(π)was 0.0043,which indicated that mtDNA genetic diversity in Gymnocypris przewalskii przewalskii is higher.

关键词: 青海湖裸鲤, mtDNA, 遗传多样性
Key words,
限制性片段长度多态性, Qinghai lake Naked carps