遗传 ›› 2001, Vol. 23 ›› Issue (5): 439-620.

• 论文 • 上一篇    下一篇

不同ES细胞系体外定量神经分化的比较

汤富酬;韩嵘;薛友纺;尚克刚 TANG Fu-chou;HAN Rong;XUE You-fang;SHANG Ke gang   

  1. 北京大学生命科学学院遗传教研室,北京 100871 College of Life Sciences ,Peking University,Beijing 100871,China
  • 收稿日期:1900-01-01 出版日期:2001-10-10 发布日期:2001-10-10

Compare of Neural Differentiation of Two ES Cell Lines

  • Received:1900-01-01 Online:2001-10-10 Published:2001-10-10

摘要: 利用视黄酸(RA)诱导小鼠胚胎干细胞通过悬浮类胚培养法体外定量分化为神经样细胞.结果显示能够进行种系嵌合的MESPU22细胞系与不能进行种系嵌合的MESPU13细胞系的体外神经分化比例在统计学上没有明显差异.此结果对于人的胚胎干细胞全能性的鉴定与检测有重要启示。
Abstract: Murine embryonic stem cells were induced to differentiate into neuron-like cells by all-trans Retinotic Acids through embryoid body culture. The results indicate that there is no obvious difference between MESPU22 cell line which is highly germline competent and MESPU13 cell line which has no germline competence. The result has important illumination on the identity of the totipotence of human embryonic stem cells.

关键词: neuronal differentiation in Vitro, RA诱导   
Key words,
ES cell line, 体外神经分化, 胚胎干细胞(ES细胞)