不同基因型毛白杨同源重组变异研究

doi:10.16288/j.yczz.20-205

遗传 ›› 2021, Vol. 43 ›› Issue (2): 182-193.doi: 10.16288/j.yczz.20-205

不同基因型毛白杨同源重组变异研究

耿喜宁^1,^2,^3,⁴(), 芦特^1,^2,³, 杜康^1,^2,³, 杨珺^1,^2,³, 康向阳^1,^2,³()

¹ 北京林业大学,北京市林木分子设计育种高精尖创新中心,北京 100083
² 北京林业大学,北京市林木分子设计育种高精尖创新中心,北京 100083
³ 北京林业大学,城乡生态环境北京实验室,北京 100083
⁴ 平顶山学院,河南省生态经济型木本植物种质创新与利用重点实验室,平顶山 467000

收稿日期:2020-07-06 出版日期:2021-02-16 发布日期:2021-01-22
基金资助:
国家自然科学基金项目(31470667)

Variation of homologous recombination in Populus tomentosa with different genotypes

Xining Geng^1,^2,^3,⁴(), Te Lu^1,^2,³, Kang Du^1,^2,³, Jun Yang^1,^2,³, Xiangyang Kang^1,^2,³()

¹ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China
² National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China
³ Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing 100083, China
⁴ Henan Province Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Woody Plant, Pingdingshan University, Pingdingshan 467000, China

Received:2020-07-06 Online:2021-02-16 Published:2021-01-22
Supported by:
the National Natural Science Foundation of China(31470667)

摘要/Abstract

摘要：

同源重组是生物遗传变异的重要来源。受检测方法限制,高等植物同源重组发生及其产物——异源双链DNA(heteroduplex DNA, hDNA)鲜有报道。本研究采用构建抑制减数后分离群体检测同源重组产物hDNA的方法,以2个母本来源的基于抑制减数后分离获得的毛白杨(Populus tomentosa)杂种三倍体群体为研究材料,利用筛选出的110个简单序列重复(Simple sequence repeat, SSR)分子标记开展毛白杨不同基因型个体间9条染色体上hDNA发生及其遗传变异研究。结果表明,2个毛白杨雌株hDNA发生频率介于8.5%~87.2%之间,且hDNA发生频率与距着丝粒距离呈正相关关系,但同一染色体平均hDNA发生频率与染色体长度无相关关系;绝大多数的染色体检测出1~3次重组事件,少数检测出4次重组事件,极少数检测到5次重组事件;不同毛白杨基因型个体间同一染色体上hDNA发生频率总体相差不大,而在一些特定SSR位点间hDNA发生频率存在较大差异;与青杨杂种‘哲引3号’杨(P. pseudo-simonii×P. nigra ‘Zheyin3#’)相比,检测到的同源重组次数及hDNA发生频率和发生位置均存在较大差异。本研究首次对2个基因型毛白杨同源重组发生特征及其变异进行了研究,为揭示高等植物同源重组特点、种间和种内同源重组差异等提供了重要见解。

关键词: 毛白杨, 基因型, 异源双链DNA, 三倍体, 同源重组

Abstract:

Homologous recombination is an important source of biological genetic variation. Limited by detection methods, there are only a few reports on the homologous recombination in high plants and its product - heteroduplex DNA (hDNA). In the present study, applying the strategy of detecting hDNA by constructing populations from inhibited post-meiotic segregation, two hybrid triploid populations were constructed from two maternal parents inPopulus tomentosa by inhibiting post-meiotic segregation. One hundred and ten simple sequence repeat (SSR) markers were used to study the occurrence and variation of hDNA on nine chromosomes inP. tomentosa with different genotypes. The results showed that the frequencies of hDNA between two female parents inP. tomentosa ranged from 8.5% to 87.2%. The hDNA frequency was positively correlated to the distance from the centromere, but the average hDNA frequency on a chromosome had no correlation with the chromosome length. One to 3 times recombination events were detected on most chromosomes, and only a few four- or five-times recombination events were detected. The overall frequencies of hDNA on the same chromosome in two genotypic individuals were roughly similar, while the hDNA frequencies varied greatly at specific SSR loci. Compared withTacamahaca poplar hybrid,P. pseudo-simonii × P. nigra ‘Zheyin3#’, detection of homologous recombination times and the frequency and location of hDNA were largely different. This study is the first to describe the characteristics and variations of homologous recombination inP. tomentosa with two different genotypes, which will provide valuable insights for exploring the characteristics and variations of homologous recombination among interspecies and intraspecies in higher plant.

Key words: Populus tomentosa, genotype, heteroduplex DNA, triploid, homologous recombination

耿喜宁, 芦特, 杜康, 杨珺, 康向阳. 不同基因型毛白杨同源重组变异研究[J]. 遗传, 2021, 43(2): 182-193.

Xining Geng, Te Lu, Kang Du, Jun Yang, Xiangyang Kang. Variation of homologous recombination in Populus tomentosa with different genotypes[J]. Hereditas(Beijing), 2021, 43(2): 182-193.

图/表 13

表1

图1

图2

图3

图4

图5

图6

附表1

鉴定hDNA的110对SSR引物信息"

标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
LG_Ⅰ-18	1	0.047	atta	F: CACGCTAAGATTGTCTCACA	53.35	45.00	320/332	320/332	320/328
				R: TCACTGCATCAAGAAGATTG	51.30	40.00
LG_Ⅰ-22	1	0.059	agcag	F: AGATAAGCATGGCTTGTTGT	51.30	40.00	309/314	309/314	309
				R: CGTTACCATTCATTCCAAAT	49.25	35.00
GCPM_2935-2	1	0.275	ttc	F: TCAGCTTCAGCTTCTTCTTC	53.35	45.00	192/204	192/204	198
				R: AATTTGAAAAGGAAAAACCC	47.20	30.00
LG_Ⅰ-1223	1	1.241	gca	F: ATATCACTCGCCAAGTCAAC	53.35	45.00	190/193	190/193	196/199
				R: ATCACGCACAAGTTAGGTTT	51.30	40.00
LG_Ⅰ-1114	1	1.424	ta	F: AAGTGGATCAGGTGATGAAG	53.35	45.00	156/166	156/166	165
				R: CAATGTCTTTCGGTTCTTTC	51.30	40.00
LG_Ⅰ-1065	1	1.753	agc	F: AATGGAGGTCCCTAGCTTAC	55.40	50.00	383/386	383/386	386
				R: TGATATTGCTGGGCTATCTT	51.30	40.00
LG_Ⅰ-1027	1	1.834	ta	F: TGAGCTTAGGAACGAAGAAG	53.35	45.00	188/198	188/198	184/186
				R: CATCAATATGACTCCTCGGT	53.35	45.00
LG_Ⅰ-1125	1	1.835	tca	F: GATGCTTACCCTTTGCTCTA	53.35	45.00	400/409	400/409	400
				R: CATAATGGGCAAAGAATACC	51.30	40.00
GCPM_3979-1	1	4.749	ga	F: TATCAATTTCCGTGGATTTC	49.25	35.00	192/196	192/196	193
				R: GAAACTGAGAGGACTTGCTG	55.40	50.00
GCPM_124	1	6.328	cac	F: TTTGAGCACTTCAACTACCA	51.30	40.00	207/210	207/210	201/210
				R: TGTCTTCCCTTAGTCACCAC	55.40	50.00
GCPM_1960-1	1	11.783	gaaa	F: TATCGAATCACAATGGCATA	49.25	35.00	220/224	220/224	220
				R: TGCTCAGGTCATCTTTTCTT	51.30	40.00
GCPM_3408	1	20.668	gt	F: ATGGAAGTTTGAGTCCACTG	53.35	45.00	202/212	202/212	200/202
				R: CATGCATTACTTCAACCCTT	51.30	40.00
GCPM_2453-1	1	28.263	ggt	F: ACACCAAGAGCTGTAGCATT	53.35	45.00	216/222	216/222	216/219
				R: ACAACATGGCCTAACTCATC	53.35	45.00
GCPM_1719	1	36.636	aaag	F: AAGTGCTCATAACATCACCC	53.35	45.00	216/228	216/228	227/234
				R: CTTTCCTCATTCCTGTTCTG	53.35	45.00
GCPM_1263-1	1	41.934	ct	F: TGCATTAAGACATCACTTGC	51.30	40.00	235/243	235/243	230/232
				R: TTCGCTTCTGTATTTCCTGT	51.30	40.00
PTSSR1686	1	42.469	ttc	F: TCAGGATCGGAGTCGGAGTT	57.45	55.00	165/168	165/168	165
				R: TGAGCAGAAACGACACCACT	55.40	50.00
GCPM_734	2	1.595	cttt	F: GGCAATTTAGGTACAACAGC	53.35	45.00	177/181	177/181	181
				R: ACAAGCGAATGCTAATTGAT	49.25	35.00
GCPM_2085-1	2	1.957	ctt	F: GCAGGAACTAACAAGGTGAG	55.40	50.00	154/157	154/157	154
				R: TCGGTAGCAATTGTCTGAAT	51.30	40.00
GCPM_3588-1	2	2.443	aaca	F: TCGACCAACTATGTTTGACA	51.30	40.00	184/184	184/184	191/195
				R: AGTTGTAACTTTGGCCTGAA	51.30	40.00

附表1

附表1(续)

鉴定hDNA的110对SSR引物信息"

标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
GCPM_1857-1	2	3.205	tat	F: TGCCAGGGTAAGAAATAAAA	49.25	35.00	196/212	196/212	196/200
				R: GAACTGAACTGATCTTGTTGC	53.66	42.86
ORPM_222	2	5.481	ag	F: TGCGAACATTTTTCTTGTGG	51.30	40.00	201/205	201/205	205
				R: CGCAATAGAGCCTTTGGATG	55.40	50.00
GCPM_2637	2	11.126	tta	F: GACACCGTTTCTTTTCTGAG	53.35	45.00	213/216	213/216	217
				R: ACCAGATCTTCATCTTCCAA	51.30	40.00
PMGC_2709	2	12.013	ga	F: ATTGTAATTATTGAACACATGCC	50.64	30.43	234/236	234/236	200/214
				R: GTGCAGTTCAGAGTATTGTTG	53.66	42.86
ORPM_161	2	13.406	ta	F: CGGGCTAGCTCAATTAGGAG	57.45	55.00	205/219	205/219	205/207
				R: ATCACCGCGAGATTCTTGTC	55.40	50.00
ORPM_459	2	14.121	att	F: TTGTGGCTGTGACCAGAAAG	55.40	50.00	227/230	227/230	233/246
				R: CGAGCCTCAGAAAATTGCTT	53.35	45.00
GCPM_2768	2	20.942	ga	F: TTATTTGGATCCTGAAATGG	49.25	35.00	196/206	186/206	168/200
				R: GATGGTTCGGTATGTGAGTT	53.35	45.00
PTSSR2445	2	23.932	ag	F: CTTTGATCCCACCAACCCCA	57.45	55.00	230/244	230/244	240/242
				R: TAGGCAGCTCTTTGACTGCA	55.40	50.00
PTSSR774	2	24.833	tga	F: TAAGGGCAAACATGGGAGGG	57.45	55.00	239/242	239/242	236/239
				R: TAGGCAGAAGTAGCAGCAGC	57.45	55.00
GCPM_2840-1	3	2.708	ta	F: GAATCCAAAACAAAACATCC	49.25	35.00	227/231	227/231	225/231
				R: AGGCATCAAAACTAATTGGA	49.25	35.00
GCPM_134-1	3	2.708	ta	F: AGGCATCAAAACTAATTGGA	49.25	35.00	227/230	227/230	225/231
				R: GAATCCAAAACAAAACATCC	49.25	35.00
GCPM_2628-1	3	4.469	agc	F: TAGGCCATGTTTTTGCTTAT	49.25	35.00	211/214	211/214	211
				R: GTGCCTCATTTATTGGTAGG	53.35	45.00
ORPM_230	3	9.739	ga	F: CTTCCCACCCCCAACATAAC	57.45	55.00	213/219	213/219	211/215
				R: TGTCATTCCACAGAGAGTCTGG	57.67	50.00
GCPM_1184-1	3	14.831	ct	F: TCTTGGCGAGAGAAGTAGAG	55.40	50.00	156/158	156/158	154/164
				R: GGATTTGGTGAAAATTGAAG	49.25	35.00
GCPM_768-1	3	16.826	cac	F: ATTCGCTTCTTCTTCCTCTT	51.30	40.00	208/211	208/211	211
				R: TATGAATTCCTCGTTGAACC	51.30	40.00
GCPM_2434-2	3	17.172	tct	F: AGAGAGAGAGGTATGAGGGC	57.45	55.00	221/224	221/224	221
				R: GTTCGGTAAAGGTGATGGA	53.01	47.37
GCPM_1468-2	3	17.480	gac	F: CAAGTCATGGCTTCTCAAAC	53.35	45.00	192/195	192/195	195/198
				R: CTATTGATCCTTGAAGACGC	53.35	45.00

GCPM_3465	3	20.996	gt	F: TCAATGATTGGTCTTGTTGA	49.25	35.00	128/134	128/134	134/146
				R: CAGAATTCAGAATAGAACCCA	51.71	38.10
GCPM_2625-1	4	0.188	gt	F: GATTTCTATTGTGGCAAAGG	51.30	40.00	187/193	187/193	187
				R: TGTATTCCTCCACTCCACTC	55.40	50.00

附表1(续)

附表1(续)

鉴定hDNA的110对SSR引物信息"

标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
GCPM_1116-1	4	0.401	tca	F: GGGGCTAATATCAGTTTCCT	53.35	45.00	195/201	195/201	195
				R: TGAGAAAACCCTGGAAAATA	49.25	35.00
ORPM_394	4	0.667	tgc	F: AAAAAGCCCCACAATTATTCA	49.76	33.33	222/225	222/225	222/231
				R: GCAAGTTGCAATTGATGTCC	53.35	45.00
GCPM_3437	4	2.481	ag	F: AAGATCTGGTGTTGTTTTGG	51.30	40.00	234/236	234/236	236/242
				R: TTCTTAACAGAAGCCAGGAG	53.35	45.00
GCPM_3971-1	4	4.122	taaa	F: CAGCCAGCCTCTTTAAGATA	53.35	45.00	193/211	193/211	202/220
				R: ACAGCAATTCTGTACCTCGT	53.35	45.00
GCPM_1297-1	4	4.758	tct	F: GGGATGAATCAGGAGATGTA	53.35	45.00	135/141	135/141	140
				R: GAAGAAACCTGTGGGTGATA	53.35	45.00
ORPM_421	4	5.650	ta	F: AAATGATGTTGCGATTTCCA	49.25	35.00	213/221	213/221	219
				R: TCCCATCTCAACTACTCCAACA	55.81	45.45
PTSSR1738	4	6.603	ctcttc	F: CTTCCTCGTTTGGCCCCTAA	57.45	55.00	277/295	277/295	289/301
				R: ACCAGCTAGTTCGGGCTTTC	57.45	55.00
ORPM_201	4	7.736	tc	F: GACTCCACCCAGTTCTGCTC	59.50	60.00	214/226	214/226	224/226
				R: AACTTCCCATCGAATGATCG	53.35	45.00
PTSSR1873	4	8.381	GA	F: GCGTGAGATGAGGGAGAGTG	59.50	60.00	184/198	184/198	186/202
				R: CGTCAGGCGACAATTCCAAC	57.45	55.00
PTSSR1932	4	9.402	TAC	F: CGGCAGGTCTGATGTCTTCA	57.45	55.00	120/123	120/123	117/123
				R: CCATGGAGTCTAAAGGTGGCA	57.57	52.38
GCPM_3819-1	4	16.315	gaa	F: ATCATCAAGTTCACGAAAGC	51.30	40.00	240/243	240/243	237
				R: ACTGACCTACCACCATTTTG	53.35	45.00
GCPM_295-1	4	16.784	aag	F: TAAGAGGATTACCAGGCAGA	53.35	45.00	136/145	136/145	142/148
				R: CCCACTAACAATAAGCTTGG	53.35	45.00
PTSSR1623	4	20.046	tga	F: CATCTCCCACCACCACAACA	57.45	55.00	238/248	238/248	238
				R: CTCTGCGTAAACAACTGCGG	57.45	55.00
PTSSR2267	4	21.767	tca	F: TGGAAGGCAAATCGTCGTCA	55.40	50.00	182/197	182/197	190
				R: AAGTGGAGCAGACAGACACT	55.40	50.00
GCPM_3234-1	4	21.772	agc	F: TCTCAATGTCGACACCATTA	51.30	40.00	195/198	195/198	195
				R: TGCAAGAACTGCTATCACTG	53.35	45.00
GCPM_1255	5	1.974	ag	F: GAACCTTAAAACCAGAACCC	53.35	45.00	191/193	191/193	189/193
				R: GAGCCACAGAAATACTGCTC	55.40	50.00
GCPM_379-1	5	4.025	tat	F: GGCCATTCATTAGATGTAGC	53.35	45.00	153/156	153/156	153
				R: AAATCAAGATCCAAAGCAAA	47.20	30.00
GCPM_1063	5	5.996	ca	F: AGTTAATTGCGCATGTTCTT	49.25	35.00	168/188	168/188	170/176
				R: AAACAAACTCCAGCAAACAT	49.25	35.00
GCPM_3536-2	5	9.139	ttg	F: AGATTCTTTTTCGGCTTCTT	49.25	35.00	137/146	137/146	141/144
				R: AGAAGATGCTGGAGTTCAGA	53.35	45.00
GCPM_1838	5	9.156	ga	F: GTTCAGCGAAAGCTAAAGAG	53.35	45.00	142/146	142/146	138/168
				R: CACAGAATTACAGCTGATGC	53.35	45.00

附表1(续)

附表1(续)

鉴定hDNA的110对SSR引物信息"

标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
GCPM_2013-1	5	14.238	At	F: TTTAGGGTCGTAGTGGAAAA	51.30	40.00	238/240	238/240	250/254
				R: AAACCTGGAGGAGAATTAGAA	51.71	38.10
ORPM_136	5	16.608	ct	F: TTTAAGCCTCCGAAAACCAA	51.30	40.00	228/240	228/240	232
				R: CTGCAAGGCGAGGTTATTCT	55.40	50.00
ORPM_442	5	16.608	ct	F: TGGTTTTCGGAGGCTTAAAA	51.30	40.00	244/254	244/254	236
				R: CCTGCAGCGAGTATTAATTGG	55.61	47.62
GCPM_3151	5	22.434	ag	F: ACCATCATTAACCCCACATA	51.30	40.00	144/156	144/156	135/155
				R: AAAGAAACCAGACCACACAC	53.35	45.00
GCPM_2858-1	5	22.702	tg	F: TGCAAGTCTTTTTAGGAACC	51.30	40.00	254/256	254/256	254/260
				R: TTCAAAATGCATCAAAGTGT	47.20	30.00
GCPM_540-1	5	24.498	taa	F: GATGGGGAGGTTATTTTCTT	51.30	40.00	154/160	154/160	154
				R: CAATATTGAGGAAAATCAAAGG	50.22	31.82
GCPM_4008-1	5	24.561	acc	F: AGAGAGAAGCTTGTGTCCAG	55.40	50.00	161/170	161/170	170
				R: TGAGGAAGCAGAAGTAGAGC	55.40	50.00
ORPM_25	5	25.091	ta	F: AAGAGTTGAAGGCTGGACGA	55.40	50.00	236/240	236/240	236
				R: AGACATGCATGAAGCCATGA	53.35	45.00
GCPM_547-1	6	1.831	at	F: CCTCTTGAAAAGAAGCAAAA	49.25	35.00	217/231	217/231	213
				R: ATCAAAAATGCCGATTAAAA	45.15	25.00
GCPM_139	6	2.516	gt	F: ATGACATGACATGATTGGAA	49.25	35.00	220/228	220/228	200/226
				R: CTTCTGCTGGAAGAAGAAAA	51.30	40.00
GCPM_2126	6	3.888	ag	F: CACGTAAACAGCTTCCAAGT	53.35	45.00	178/194	178/194	160/176
				R: TAATGATTCCAGCTATGGGT	51.30	40.00
GCPM_1072-2	6	4.204	ttta	F: AGGAAAACAAAGGAGAGGAG	53.35	45.00	150/154	150/154	142/154
				R: ATGCTTAAAAGGGGATCTCT	51.30	40.00
GCPM_3539-2	6	5.331	aat	F: TATTCGGTACAAGACTTGGG	53.35	45.00	215/218	215/218	212/215
				R: TAATTGTAAAGCGGCTATCG	51.30	40.00
GCPM_2642-2	6	6.965	cca	F: GCTTAGCTGGATGAGAAGAA	53.35	45.00	239/241	239/241	239
				R: ATAGTTACAGGCCACCATTG	53.35	45.00
PTSSR580	6	13.716	atg	F: AATGCATCCTCAGCTCCAGG	57.45	55.00	281/284	281/284	281
				R: CGGCGGCTGCAAACATTAAT	55.40	50.00
GCPM_1017-1	6	22.438	ataa	F: GTTTAATTCCCACGTCGTTA	51.30	40.00	171/175	171/175	175/177
				R: CGAATGAAGAAAAACCATTC	49.25	35.00
GCPM_2705-1	6	22.633	ac	F: TTTGCACAGGTAAGTTGATG	51.30	40.00	146/150	146/150	148/152
				R: ATTGACCATAGCAGACAACC	53.35	45.00
GCPM_2034	6	23.087	ag	F: ACAAACTGCTTTGTTTGGTT	49.25	35.00	176/190	176/190	186/196
				R: CTCCATTCATAAAATCGAGC	51.30	40.00
GCPM_1632-1	6	23.663	ct	F: TTTCTCTCTCTGAAACCCCT	53.35	45.00	211/225	211/225	203/221
				R: AGCGACTCACTGAGCTTTAG	55.40	50.00
ORPM_365	6	23.788	tg	F: GGGTTGGACCTGCTCAAATA	55.40	50.00	226/228	226/228	226/238
				R: CAGCTGCTTTTTCATGGCTA	53.35	45.00

附表1(续)

附表1(续)

鉴定hDNA的110对SSR引物信息"

标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
GCPM_2627-1	6	24.930	cac	F: TAAGTCCCACTACACCCAAC	55.40	50.00	234/254	234/254	240/255
				R: GAGTTCGAGAGAGGGAATCT	55.40	50.00
GCPM_2615-1	6	27.782	cttt	F: ATGTCAACGTCACTGACAAA	51.30	40.00	231/237	231/237	229/236
				R: ATTAGGCAATGCAGAACACT	51.30	40.00
GCPM_1054-1	7	0.863	ct	F: AGGTCTGTGCAAGGAATAAA	51.30	40.00	159/165	159/165	154/165
				R: GTCTGTAATCAAGCCAAAGC	53.35	45.00
GCPM_741-1	7	1.416	at	F: CCGTTTGATTAAAAAGATGC	49.25	35.00	212/214	212/214	214
				R: TTATTGAGCTGATGATCCCT	51.30	40.00
WPMS_17	7	6.495	cac	F: ACATCCGCCAATGCTTCGGTGTTT	59.57	50.00	137/146	137/146	137/140
				R: GTGACGGTGGTGGCGGATTTTCTT	61.28	54.17
GCPM_3738-1	7	9.014	ac	F: TGAACAAGACACCAAAATGA	49.25	35.00	133/139	133/139	133
				R: TCTCGACTTTACCATCTCGT	53.35	45.00
GCPM_2741-1	7	12.076	at	F: CAAGCAGTATCTTCCACTGA	53.35	45.00	142/146	142/148	134
				R: TAGCCAACCACTCTAAAGGA	53.35	45.00
GCPM_3476-1	7	12.189	ggt	F: GGGAATGTAAGGATGTGTTG	53.35	45.00	202/220	202/220	205/211
				R: AACCAGAAAACGACAGTCAC	53.35	45.00
GCPM_3332-1	7	13.116	ag	F: TCCACTGCCTATGAACTTTT	51.30	40.00	138/154	138/154	138
				R: CACCCAATAGCTTCCATATT	51.30	40.00
GCPM_3727-1	7	13.848	ct	F: TTGGGGTTAGTGACTAGTGG	55.40	50.00	152/158	152/158	152/154
				R: CAAGCTGTGTAAAGACACCA	53.35	45.00
LG_Ⅷ-50	8	0.255	ga	F: AACAACAGATCGCTGAAACT	51.30	40.00	265/281	265/281	265/271
				R: CCATGTACTTAGGCATGGAT	53.35	45.00
GCPM_2992	8	1.833	TA	F: GATCATAACCCAGGAAACAA	51.30	40.00	237/241	237/241	241
				R: CGTGAATTTTTGGGATTTTA	47.20	30.00
GCPM_1344-2	8	3.669	tat	F: GAGACCTGAGAAACAGAGGA	55.40	50.00	227/230	227/230	230
				R: CAAATGCAACGCAATAATAA	47.20	30.00
GCPM_33-1	8	4.192	tta	F: TTTGGCCTTAACTCTCCATA	51.30	40.00	171/174	171/174	167/179
				R: AGTTGCTCTCAAAGAAGGTT	51.30	40.00
GCPM_13-1	8	7.309	taa	F: ATTGTTCTTGTTGAAGGACG	51.30	40.00	207/213	207/213	204/210
				R: AGAGCAAACAAATTGATGGT	49.25	35.00
ORPM_126	8	11.293	[ct]4cct	F: GCCGAAGTTGACGATAGCTC	57.45	55.00	215/222	215/222	215
				R: GAGTTAAACCCACCCTGCAA	55.40	50.00
ORPM_455	8	11.293	ga	F: GAGTTAAACCCACCCTGCAA	55.40	50.00	215/225	215/225	215
				R: GCCGAAGTTGACGATAGCTC	57.45	55.00
GCPM_2958-1	8	11.639	ct	F: AAAGAGGATTACTTAGGCGG	53.35	45.00	201/215	201/215	217/219
				R: ACGAGAACATTCATCAATCC	51.30	40.00
GCPM_3474-1	8	16.814	agc	F: GATCCGAAAACAACAACAAT	49.25	35.00	123/135	123/135	123
				R: ACCCCTTTCTCTTCTCAATC	53.35	45.00

附表1(续)

参考文献 43

[1]	Cromie GA, Smith GR. Branching out: meiotic recombination and its regulation. Trends Cell Biol , 2007, 17(9): 448-455. Homologous recombination is a dynamic process by which DNA sequences and strands are exchanged. In meiosis, the reciprocal DNA recombination events called crossovers are central to the generation of genetic diversity in gametes and are required for homolog segregation in most organisms. Recent studies have shed light on how meiotic crossovers and other recombination products form, how their position and number are regulated and how the DNA molecules undergoing recombination are chosen. These studies indicate that the long-dominant, unifying model of recombination proposed by Szostak et al. applies, with modification, only to a subset of recombination events. Instead, crossover formation and its control involve multiple pathways, with considerable variation among model organisms. These observations force us to ‘branch out’ in our thinking about meiotic recombination. doi: 10.1016/j.tcb.2007.07.007
[2]	Melamed-Bessudo C, Shilo S, Levy AA. Meiotic recombination and genome evolution in plants. Curr Opin Plant Biol , 2016, 30: 82-87.
[3]	Mancera E, Bourgon R, Huber W, Steinmetz LM. Genome-wide survey of post-meiotic segregation during yeast recombination. Genome Biol , 2011, 12(4): R36. doi: 10.1186/gb-2011-12-4-r36 pmid: 21481229
[4]	Martini E, Borde V, Legendre M, Audic S, Regnault B, Soubigou G, Dujon B, Llorente B. Genome-wide analysis of heteroduplex DNA in mismatch repair-deficient yeast cells reveals novel properties of meiotic recombination pathways. PLoS Genet , 2011, 7(9): e1002305. doi: 10.1371/journal.pgen.1002305 pmid: 21980306
[5]	Preuss D, Rhee SY, Davis RW. Tetrad analysis possible in Arabidopsis with mutation of the quartet ( qrt ) genes . Science , 1994, 264(5164): 1458-1460. pmid: 8197459
[6]	Francis KE, Lam SY, Harrison BD, Bey AL, Berchowitz LE, Copenhaver GP. Pollen tetrad-based visual assay for meiotic recombination in Arabidopsis . Proc Natl Acad Sci USA , 2007, 104(10): 3913-3918.
[7]	Sun YJ, Ambrose JH, Haughey BS, Webster TD, Pierrie SN, Muñoz DF, Wellman EC, Cherian S, Lewis SM, Berchowitz LE, Copenhaver GP. Deep genome-wide measurement of meiotic gene conversion using tetrad analysis in Arabidopsis thaliana . PLoS Genet , 2012, 8(10): e1002968.
[8]	Berchowitz LE, Copenhaver GP. Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets . Nat Protoc , 2008, 3(1): 41-50. doi: 10.1038/nprot.2007.491 pmid: 18193020
[9]	Yelina NE, Ziolkowski PA, Miller N, Zhao XH, Kelly KA, Muñoz DF, Mann DJ, Copenhaver GP, Henderson IR. High-throughput analysis of meiotic crossover frequency and interference via flow cytometry of fluorescent pollen in Arabidopsis thaliana . Nat Protoc , 2013, 8(11): 2119-2134. doi: 10.1038/nprot.2013.131 pmid: 24113785
[10]	Li X, Li L, Yan JB. Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize. Nat Commun , 2015, 6: 6648. pmid: 25800954
[11]	Dreissig S, Fuchs J, Himmelbach A, Mascher M, Houben A. Sequencing of single pollen nuclei reveals meiotic recombination events at megabase resolution and circumvents segregation distortion caused by postmeiotic processes . Front Plant Sci , 2017, 8: 1620. doi: 10.3389/fpls.2017.01620 pmid: 29018459
[12]	Dong CB, Mao JF, Suo YJ, Shi L, Wang J, Zhang PD, Kang XY. A strategy for characterization of persistent heteroduplex DNA in higher plants. Plant J , 2014, 80(2): 282-291.
[13]	Bauer E, Falque M, Walter H, Bauland C, Camisan C, Campo L, Meyer N, Ranc N, Rincent R, Schipprack W, Altmann T, Flament P, Melchinger AE, Menz M, Moreno-González J, Ouzunova M, Revilla P, Charcosset A, Martin OC, Schön CC. Intraspecific variation of recombination rate in maize. Genome Biol , 2013, 14(9): R103.
[14]	Yin TM, Difazio SP, Gunter LE, Zhang XY, Sewell MM, Woolbright SA, Allan GJ, Kelleher CT, Douglas CJ, Wang MX, Tuskan GA. Genome structure and emerging evidence of an incipient sex chromosome in Populus . Genome Res , 2008, 18(3): 422-430. doi: 10.1101/gr.7076308 pmid: 18256239
[15]	Kersten B, Pakull B, Fladung M. Genomics of sex determination in dioecious trees and woody plants. Trees , 2017, 31(4): 1113-1125.
[16]	Kang N, Bai FY, Zhang PD, Kang XY. Inducing chromosome doubling of embryo sac in Populus tomentosa with high temperature exposure for hybrid triploids . J Beijing Fore Univ , 2015, 37(2): 79-86.
	康宁, 白凤莹, 张平冬, 康向阳. 高温诱导胚囊染色体加倍获得毛白杨杂种三倍体. 北京林业大学学报, 2015, 37(2): 79-86.
[17]	Li YH. Chromosome doubling of female gametes in white poplars[Dissertation]. Beijing Forstry University, 2007.
	李艳华. 白杨雌配子染色体加倍技术研究[学位论文]. 北京林业大学, 2007.
[18]	Wang J. Techniques of polyploid induction in Populus spp. (Section Tacamahaca )[Dissertation]. Beijing Forstry University, 2009.
	王君. 青杨派树种多倍体诱导技术研究[学位论文]. 北京林业大学, 2009.
[19]	Schuelke M. An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol , 2000, 18(2): 233-234. doi: 10.1038/72708 pmid: 10657137
[20]	Hulce D, Li X, Snyder-Leiby T, Johathan LC. GeneMarker® Genotyping Software: Tools to increase the statistical power of dna fragment analysis. J Biomol Tech , 2011,22(Suppl.): S35-S36.
[21]	Yin TM, Zhang XY, Gunter LE, Li SX, Wullschleger SD, Huang MR, Tuskan GA. Microsatellite primer resource for Populus developed from the mapped sequence scaffolds of the Nisqually-1 genome . New Phytol , 2009, 181(2): 498-503. doi: 10.1111/j.1469-8137.2008.02663.x pmid: 19121044
[22]	Vining KJ, Pomraning KR, Wilhelm LJ, Priest HD, Pellegrini M, Mockler TC, Freitag M, Strauss SH. Dynamic DNA cytosine methylation in the Populus trichocarpa genome: tissue-level variation and relationship to gene expression . BMC Genomics , 2012, 13: 27. doi: 10.1186/1471-2164-13-27 pmid: 22251412
[23]	Xin HY, Zhang T, Han YH, Wu YF, Shi JS, Xi ML, Jiang JM. Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides . Chromosoma , 2018, 127(3): 313-321. doi: 10.1007/s00412-018-0664-y pmid: 29520650
[24]	Si WN, Yuan Y, Huang J, Zhang XH, Zhang YC, Zhang YD, Tian DC, Wang CL, Yang YH, Yang SH. Widely distributed hot and cold spots in meiotic recombination as shown by the sequencing of rice F ₂ plants . New Phytol , 2015, 206(4): 1491-1502.
[25]	Wijnker E, Velikkakam James G, Ding J, Becker F, Klasen JR, Rawat V, Rowan BA, de Jong DF, de Snoo CB, Zapata L, Huettel B, de Jong H, Ossowski S, Weigel D, Koornneef M, Keurentjes JJ, Schneeberger K. The genomic landscape of meiotic crossovers and gene conversions in Arabidopsis thaliana . eLife , 2013, 2: e01426. doi: 10.7554/eLife.01426 pmid: 24347547
[26]	Henderson IR. Control of meiotic recombination frequency in plant genomes. Curr Opin Plant Biol , 2012, 15(5): 556-561. pmid: 23017241
[27]	Yelina N, Diaz P, Lambing C, Henderson IR. Epigenetic control of meiotic recombination in plants. Sci China Life Sci , 2015, 58(3): 223-231. doi: 10.1007/s11427-015-4811-x pmid: 25651968
[28]	Kleckner N, Storlazzi A, Zickler D. Coordinate variation in meiotic pachytene SC length and total crossover/ chiasma frequency under conditions of constant DNA length. Trends Genet , 2003, 19(11): 623-628. doi: 10.1016/j.tig.2003.09.004 pmid: 14585614
[29]	Ruiz-Herrera A, Vozdova M, Fernández J, Sebestova H, Capilla L, Frohlich J, Vara C, Hernández-Marsal A, Sipek J, Robinson TJ, Rubes J. Recombination correlates with synaptonemal complex length and chromatin loop size in bovids-insights into mammalian meiotic chromosomal organization. Chromosoma , 2017, 126(5): 615-631. doi: 10.1007/s00412-016-0624-3 pmid: 28101670
[30]	Quevedo C, Del Cerro AL, Santos JL, Jones GH. Correlated variation of chiasma frequency and synaptonemal complex length in Locusta migratoria . Heredity , 1997, 78(5): 515-519.
[31]	Lynn A, Koehler KE, Judis L, Chan ER, Cherry JP, Schwartz S, Seftel A, Hunt PA, Hassold TJ. Covariation of synaptonemal complex length and mammalian meiotic exchange rates. Science , 2002, 296(5576): 2222-2225.
[32]	Anderson LK, Stack SM, Fox MH, Zhang CS. The relationship between genome size and synaptonemal complex length in higher plants. Exp Cell Res , 1985, 156(2): 367-378. doi: 10.1016/0014-4827(85)90544-0 pmid: 3967684
[33]	Giraut L, Falque M, Drouaud J, Pereira L, Martin OC, Mézard C. Genome-wide crossover distribution in Arabidopsis thaliana meiosis reveals sex-specific patterns along chromosomes . PLoS Genet , 2011, 7(11): e1002354. doi: 10.1371/journal.pgen.1002354 pmid: 22072983
[34]	Basheva EA, Bidau CJ, Borodin PM. General pattern of meiotic recombination in male dogs estimated by MLH1 and RAD51 immunolocalization. Chromosome Res , 2008, 16(5): 709-719.
[35]	Mancera E, Bourgon R, Brozzi A, Huber W, Steinmetz LM. High-resolution mapping of meiotic crossovers and non- crossovers in yeast. Nature , 2008, 454(7203): 479-485.
[36]	Page SL, Hawley RS. Chromosome choreography: the meiotic ballet. Science , 2003, 301(5634): 785-789. pmid: 12907787
[37]	Jones GH. The control of chiasma distribution. Symp Soc Exp Biol , 1984, 38: 293-320. pmid: 6545727
[38]	Ritz KR, Noor MAF, Singh ND. Variation in recombination rate: adaptive or not? Trends Genet , 2017, 33(5): 364-374. doi: 10.1016/j.tig.2017.03.003 pmid: 28359582
[39]	Drouaud J, Mercier R, Chelysheva L, Bérard A, Falque M, Martin O, Zanni V, Brunel D, Mézard C. Sex-specific crossover distributions and variations in interference level along Arabidopsis thaliana chromosome 4 . PLoS Genet , 2007, 3(6): e106. doi: 10.1371/journal.pgen.0030106 pmid: 17604455
[40]	Luo C, Li X, Zhang QH, Yan JB. Single gametophyte sequencing reveals that crossover events differ between sexes in maize. Nat Commun , 2019, 10(1): 785. pmid: 30770831
[41]	Dong CB. Analysis of homologous recombination characteristics and 2n gamete heterozygosity in Populus [Dissertation]. Beijing Forestry University, 2015.
	董春波. 杨树同源重组特点及2n配子杂合性分析[学位论文]. 北京林业大学, 2015.
[42]	Yang SH, Yuan Y, Wang L, Li J, Wang W, Liu HX, Chen JQ, Hurst LD, Tian DC. Great majority of recombination events in Arabidopsis are gene conversion events . Proc Natl Acad Sci USA , 2012, 109(51): 20992-20997.
[43]	Hadad RG, Pfeiffer TW, Poneleit CG. Repeatability and heritability of divergent recombination frequencies in the Iowa Stiff Stalk Synthetic ( Zea mays L. ). Theor Appl Genet , 1996, 93( 5-6): 990-996.

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项目	Chr.01	Chr.02	Chr.03	Chr.04	Chr.05	Chr.06	Chr.07	Chr.08	Chr.19
染色体长度(Mb)	50.5	25.3	21.8	24.3	25.9	27.9	15.6	19.5	15.9
标记数量	16	12	9	16	13	14	8	9	13

标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
标记名称	染色体	位置 (Mb)	重复单元	引物序列(5?→3?)	Tm (℃)	GC含量(%)	3532中的等位基因(bp)	3119中的等位基因(bp)	YX1中的等位基因(bp)
MB50065	19	0.301	A	F: CACCAAAATTAAAGGGCCAA			204/218	204/218	201
				R: GCATGCTAGTGCCTTTTCCT
MB41376	19	1.940	at	F: GAGCAGGTGACTCGTGAACA			251/255	251/255	251
				R: GGAGAGTGATAAAACCAACAATCC
MB41583	19	2.580	gcg	F: CAAGTTGGCTTGGATGGATT			144/162	144/162	146/149
				R: AACCAGCCCAACAAATCAAG
MB55349	19	3.680	tc	F: GGACACGTGATGTCATGGAG			264/268	264/268	258/260
				R: TCAAATGCAAAGCCCTTTTT
MB54907	19	4.713	ga	F: TAGCAAAAGCAGCAGCAGAG			262/300	262/300	262/378
				R: CAAGTGCGTGAAAAACATGG
MB54763	19	5.159	tta	F: AGTTGAATTTGCGCTTCGAT			128/134	128/134	128
				R: TAGGGTGTGGCCAACATACA
MB84322	19	5.526	tta	F: GCATGCTGTTTGCATCTAAAG			234/248	234/248	234
				R: ATTAAACCTCGGGTCCTGGT
MB120414	19	7.927	tg	F: GTGGCGAAGCTTTATTCCTG			297/311	297/311	307/311
				R: TAAAGCCCAAAGCAGTCACC
LG_XIX-11	19	8.770	atca	F: CAACATGAAATGAGCTGCTA			398/404	398/404	404/408
				R: TCCACATGATGTCTGATTTG
MB125350	19	9.649	tc	F: AAAGAGTCAACGGGCAAAAA			284/290	284/290	292/300
				R: AGTGGCAGCTGAATGGAAAT
MB37553	19	10.266	ta	F: TGACCAAGAGATTGCCACAG			298/306	298/306	317/343
				R: GAAAGTTTCATGTGCCGGAT
MB125779	19	11.237	t	F: TTGAAGTATGATTAGCATCCTCG			112/115	112/115	115/123
				R: TCCCTCTGGTTACCATTTGC
MB70130	19	15.319	a	F: TGCTCCAGAGGTTACTCCGT			233/239	233/239	233
				R: TCCAATGATGGATCTGGTGA

不同基因型毛白杨同源重组变异研究

Variation of homologous recombination in Populus tomentosa with different genotypes

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