细菌Cpx信号转导系统的功能及调控机制研究进展

doi:10.16288/j.yczz.21-208

遗传 ›› 2021, Vol. 43 ›› Issue (8): 747-757.doi: 10.16288/j.yczz.21-208

细菌Cpx信号转导系统的功能及调控机制研究进展

吴丽雯(), 曾洁, 薛云新, 赵西林()

厦门大学公共卫生学院,分子疫苗学和分子诊断学国家重点实验室,厦门 361102

收稿日期:2021-06-10 修回日期:2021-07-14 出版日期:2021-08-20 发布日期:2021-07-23
通讯作者: 赵西林 E-mail:wlw6621@126.com;zhaox5@xmu.edu.cn
作者简介:吴丽雯,在读硕士研究生,专业方向：公共卫生。E-mail: wlw6621@126.com
基金资助:
国家自然科学基金面上项目编号(81971905);福建省海洋经济发展补助资金项目资助编号(FJHJF-L-2019-4)

Progress on the function and regulatory mechanisms of bacterial Cpx signal transduction system

Liwen Wu(), Jie Zeng, Yunxin Xue, Xilin Zhao()

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China

Received:2021-06-10 Revised:2021-07-14 Online:2021-08-20 Published:2021-07-23
Contact: Zhao Xilin E-mail:wlw6621@126.com;zhaox5@xmu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China No(81971905);Marine Economy Development Subsidy Foundation of Fujian Province of China No(FJHJF-L-2019-4)

摘要/Abstract

摘要：

Cpx (conjugative pilus expression)双组分信号转导系统是革兰阴性细菌中一种复杂的包膜应激系统,能感应从不同信号传输点传入的多种包膜信号。位于胞质中的反应调节子CpxR磷酸化后能够调节众多编码内外膜上相关蛋白基因的表达。Cpx系统的激活还能调节细菌对抗生素和酸等压力的抵抗性。本文介绍了Cpx系统的组成,重点对Cpx系统的信号感应及调控机制进行综述,以期为Cpx系统的调控网络及其调节细菌重要生理过程的研究提供参考依据。

关键词: Cpx双组分系统, 包膜应激反应, 抗生素耐药, 酸应激

Abstract:

The Cpx (conjugative pilus expression) two-component signal transduction system is a complex envelope stress response system in Gram-negative bacteria, which can sense a variety of extracellular stimuli that enter the signaling pathway at different points. The phosphorylation of the CpxR, the cytoplasmic cognate response regulator of the Cpx system, can lead to changes in the expression of genes encoding proteins involved in inner and outer membrane functions. Activation of the Cpx system contributes to bacterial resistance/tolerance to certain antibiotics and acidic stress. In this review, we summarize the composition, and the mechanisms of signal detection, and the transcriptional regulation of the Cpx system, with a goal to provide guidance for the study of the regulatory network of the Cpx system and its important regulatory roles in bacterial physiology.

Key words: Cpx two-component system, envelope stress responses, antibiotic resistance, acid stress

吴丽雯, 曾洁, 薛云新, 赵西林. 细菌Cpx信号转导系统的功能及调控机制研究进展[J]. 遗传, 2021, 43(8): 747-757.

Liwen Wu, Jie Zeng, Yunxin Xue, Xilin Zhao. Progress on the function and regulatory mechanisms of bacterial Cpx signal transduction system[J]. Hereditas(Beijing), 2021, 43(8): 747-757.

图/表 2

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细菌种类	抗生素耐药性	啊啊啊啊
大肠杆菌(Escherichia coli)	氨基糖苷类、β-内酰胺类、杀菌肽聚糖、羟基脲、阳离子抗菌肽、细胞透膜肽(Cell penetrating peptide, CPP)	[13,14,64~67]
肠出血性大肠杆菌O157:H7 (Enterohaemorrhagic Escherichia coli O157:H7)	磷霉素	[68]
肺炎克雷伯菌(Klebsiella pneumoniae)	β-内酰胺类、氯霉素、利福平、四环素、链霉素、红霉素	[63,69]
鼠伤寒沙门氏菌(Salmonella typhimurium)	氨基糖苷类、β-内酰胺类	[70,71]
产气克雷伯氏菌(Klebsiella aerogenes)	β-内酰胺类	[12,72~74]
产气肠杆菌(Enterobacter aerogenes)
铜绿假单胞菌(Pseudomonas aeruginosa)
霍乱弧菌(Vibrio cholerae)
副猪嗜血杆菌(Haemophilus parasuis)	大环内酯类	[75]

细菌Cpx信号转导系统的功能及调控机制研究进展

Progress on the function and regulatory mechanisms of bacterial Cpx signal transduction system

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