遗传 ›› 2002, Vol. 24 ›› Issue (1): 31-34.
朱嘉明;刘飞鹏;李月琴;钟振宇;张娜 ZHU Jia-ming;LIU Fei-peng;LI Yue-qing;ZHONG Zhen-yu;ZHANG Na
摘要: 根据天蚕抗菌肽A(cecropin A,CA)N端第1~7个 氨基酸残基、 蜂毒素(melittin,M)N端第5~12个氨基酸残基,以大肠杆菌偏爱的密码子设计合成了杂合肽CA(1~7)-M(5~12)基因,同载体pGEMEX-1连接后转化大肠杆菌JM109,经打点杂交筛选和序列测定,获得一个与设计一致的克隆。将此克隆中的质粒亚克隆至JM103(DE3) ,经IPTG诱导、BrCN切割和抑菌圈试验表明,克隆的杂合肽基因在JM109(DE3)中获得了表达。 Abstract:According to the studies of David Andreu and other scientists,we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid.This hybrid consists of CA(1-7) and M(5-12).The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI.A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).