PCR反应混合液的冻干处理及其应用

遗传 ›› 2002, Vol. 24 ›› Issue (2): 171-173.

PCR反应混合液的冻干处理及其应用

汪秀峰;李莉;皮桃花;倪大虎;张毅;向太和;杨剑波 WANG Xiu-feng;LI Li;PI Tao-hua;NI Da-hu;ZHANG Yi;XIANG Tai-he;YANG Jian-bo

安徽省农业科学院农业部水稻遗传育种重点开放实验室，合肥 230031 Key Laboratory of Rice Genetics and Breeding for Agricultural Ministry of China,Anhui Academy of Agricultural Sciences,Hefei 230031

收稿日期:1900-01-01 出版日期:2002-04-10 发布日期:2002-04-10

Lyophilized Mixture of PCR Ingredients and Its Application

Received:1900-01-01 Online:2002-04-10 Published:2002-04-10

摘要/Abstract

摘要： 本研究将除模板DNA以外的PCR反应混合液，经过真空浓缩冻成干粉状后，分别置于室温和4℃贮存，经过一定的贮藏时间后进行PCR反应，发现经冻干处理的样品能在较长的贮存时间内保持扩增活性，这将给实际工作带来很大的便利。
Abstract:In this paper we introduce a new storage method of PCR ingredient.PCR mixture except DNA template has been frozen to dry powder by the DNA-Plus system.This kind of powder was stored at room temperature or 4℃.PCR has been run in different period of storage.It was discovered that the samples of lyophilization could keep activity for a long time.

关键词: 冻干, 贮存, storage, lyophilization, PCR扩增
Key words

汪秀峰，李莉，皮桃花，倪大虎，张毅，向太和，杨剑波. PCR反应混合液的冻干处理及其应用[J]. 遗传, 2002, 24(2): 171-173.

WANG Xiu-feng, LI Li, PI Tao-hua, NI Da-hu, ZHANG Yi, XIANG Tai-he, YANG Jian-bo. Lyophilized Mixture of PCR Ingredients and Its Application[J]. HEREDITAS, 2002, 24(2): 171-173.

[1]	陈豫，曲乐庆，贾旭. 水稻种子储藏蛋白及其基因表达[J]. 遗传, 2003, 25(3): 367-372.
[2]	赵世民，徐金相，刘根齐，刘承宪，李雪梅. 胡萝卜与烟草原生质体的低温保存[J]. 遗传, 1997, 19(6): 18-20.