遗传 ›› 2003, Vol. 25 ›› Issue (3): 327-329.
赵春江;李宁;邓学梅 ZHAO Chun-Jiang;LI Ning;DENG Xue-Mei
摘要: 应用引物错配技术结合单碱基突变位点而配合成一个酶切位点,使之成为可用PCR-RFLP方法分析的突变位点,是对单碱基突变位点进行基因型鉴定的有效而简捷的手段。本文以鸡胞外脂肪酸结合蛋白(Extracelluar fatty acid binding protein,EX-FABP)基因单碱基突变的基因型检测为例,探讨了应用创造酶切位点PCR(Created Restriction Site PCR,CRS-PCR)检测单碱基突变基因型的思路、方法和策略。
Abstract:Created Restriction Site PCR (CRS-PCR) is a simple and efficient method to identify SNP genotypes.One or more mismatch bases are used in a primer to create a restriction site by combining SNP site after PCR.The CRS-PCR products can be genotyped with a way the same as PCR-RFLP.In the study,Extracelluar fatty acid binding protein (EX-FABP) gene was served as an example for establishing the CRS-PCR method.Strategy of CRS-PCR was also discussed.