遗传 ›› 2004, Vol. 26 ›› Issue (1): 69-74.
贺治国1;胡岳华1;胡维新2;钟慧2;徐兢1;朱敏2 HE Zhi-Guo1;HU Yue-Hua1;HU Wei-Xin2;ZHONG Hui2;XU Jing1;Zhu Min2
摘要:
本文对采自7个不同环境的氧化亚铁硫杆菌进行了随机扩增多态性DNA(RAPD)分析,20个引物中筛选出扩增效果较好的4个引物,每个引物能产生1~9条DNA条带。通过4个引物的RAPD分析获得的平均相似性系数表明不同来源的菌之间的相关系数在44%~83%之间。
Abstract:Random amplified polymorphic DNA(RAPD) was used in analyzing the polymorphisms of Thiobacillus ferrooxidans from seven different places.Of the 20 primers,four could generate reproducible RAPD profiles,and each one produced 1~9 bands.The similarity coefficients obtained from profiles generated by four primers among Thiobacillus Ferrooxidans were about 44%~83%.