遗传 ›› 2004, Vol. 26 ›› Issue (1): 69-74.

• 论文 • 上一篇    下一篇

用RAPD分子标志方法研究氧化亚铁硫杆菌遗传多样性

贺治国1;胡岳华1;胡维新2;钟慧2;徐兢1;朱敏2 HE Zhi-Guo1;HU Yue-Hua1;HU Wei-Xin2;ZHONG Hui2;XU Jing1;Zhu Min2   

  1. 1.中南大学资源加工与生物工程学院, 长沙 410083; 2.中南大学湘雅医学院分子生物学研究中心,长沙 410078 1.School of Resource Process and Bioengineering,CSU,Changsha 410083,China; 2.Molecular Biology Research Centre,Medical School of Xiangya,CSU,Changsha 410078,China
  • 收稿日期:1900-01-01 出版日期:2004-02-10 发布日期:2004-02-10

Studies of Polymorphisms of Thiobacillus Ferrooxidans Using RAPD

  • Received:1900-01-01 Online:2004-02-10 Published:2004-02-10

摘要:
本文对采自7个不同环境的氧化亚铁硫杆菌进行了随机扩增多态性DNA(RAPD)分析,20个引物中筛选出扩增效果较好的4个引物,每个引物能产生1~9条DNA条带。通过4个引物的RAPD分析获得的平均相似性系数表明不同来源的菌之间的相关系数在44%~83%之间。
Abstract:Random amplified polymorphic DNA(RAPD) was used in analyzing the polymorphisms of Thiobacillus ferrooxidans from seven different places.Of the 20 primers,four could generate reproducible RAPD profiles,and each one produced 1~9 bands.The similarity coefficients obtained from profiles generated by four primers among Thiobacillus Ferrooxidans were about 44%~83%.


关键词: RAPD, Thiobacillus ferrooxidans, 亲缘关系
Key words,
RAPD, 氧化亚铁硫杆菌