基于IPA分析自噬对大鼠肝再生中树突状细胞的调节作用

doi:10.16288/j.yczz.14-391

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HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (3): 276-282.doi: 10.16288/j.yczz.14-391

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Regulation of autophagy on dendritic cells during rat liver regeneration by IPA

Qiwen Wang^{1, 2, 3}, Wei Jin^{1, 2, 3}, Cuifang Chang^{1, 2, 3}, Cunshuan Xu^{1, 2, 3}

1. Key Laboratory for Bioengineering, Henan Normal University, Xinxiang 453007, China;
2. Co-construction Key Laboratory for Cell Differentiation and Regulation, Henan Normal University, Xinxiang 453007, China;
3. College of Life Science, Henan Normal University, Xinxiang 453007, China

Received:2014-11-13 Revised:2015-01-12 Online:2015-03-20 Published:2015-02-10

Abstract

Abstract: To understand the mechanism underlying autophagy in regulating dendritic cells during rat liver regeneration, we used the method of percoll density gradient centrifugation combined with immunomagnetic bead to isolate dendritic cells, the Rat Genome 230 2.0 Array to determine the expression changes of autophagy-related genes, and Ingenuity Pathway Analysis 9.0 (IPA) to determine the autophagy activities. The results indicated that LC3, BECN1, ATG7 and SQSTM1 genes had significant expression changes during rat liver regeneration. There were 593 genes related to autophagy, among which 210 genes were identified as significant. We also showed that the activity of autophagy was enhanced in the priming phase and teminal phase of liver regeneration, weakened in the proliferative stage by comparative analysis method of IPA. The autophagy-related physiological activities mainly included RNA expression, RNA transcription, cell differentiation and proliferation, involving in PPARα/RXRα activation, acute phase response signaling, TREM1 signaling, IL-6 signaling, IL-8 signaling and IL-1 signaling, whose activities were increased or decreased in liver regeneration. Cluster analysis found that P53 and AMPK signaling participated in the regulation of dendritic cells autophagy, with AMPK signaling in the priming phase of liver regeneration, and both signaling pathways in the terminal phase. We conclude that dendritic cells autophagy played an important role in initiation of the immune response in priming phase and depletion of dendritic cells in late phase during rat liver regeneration.

Key words: rat liver regeneration, autophagy, dendritic cells, Rat Genome 230 2.0 Array, ingenuity pathway analysis

Qiwen Wang, Wei Jin, Cuifang Chang, Cunshuan Xu. Regulation of autophagy on dendritic cells during rat liver regeneration by IPA[J]. HEREDITAS(Beijing), 2015, 37(3): 276-282.

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Regulation of autophagy on dendritic cells during rat liver regeneration by IPA

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