腹部脂肪组织APN基因DNA甲基化及mRNA表达与维吾尔族T2DM的相关性

doi:10.16288/j.yczz.14-199

[an error occurred while processing this directive]

HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (3): 269-275.doi: 10.16288/j.yczz.14-199

• Research Articles • Previous Articles Next Articles

Correlation between type 2 diabetes and DNA methylation and mRNA expression of APN in abdominal adipose tissues in Xinjiang Uygur population

Jun Zhang¹, Wangqiang Zhang², Yulei DING¹, Peng Xu¹, Tingting WANG¹, Wenjing Xu¹, Huan Lu¹, Zongzhi Liu¹, Jianxin Xie¹

1. The Key Laboratory of Xinjiang Endemic and Ethnic Disease, Ministry of Education , Medicine School of Shihezi University, Shihezi 832000,China;
2. Department of Cardiology, First Affiliated Hospital, Medicine School of Shihezi University, Shihezi 832000,China

Received:2014-06-19 Revised:2014-10-11 Online:2015-03-20 Published:2015-02-10

Abstract

Abstract: To investigate the relationship between type 2 diabetes mellitus (T2DM) onset and development and mRNA expression and promoter methylation of adiponectin (APN) gene in abdominal adipose tissues of Xinjiang Uygur population, abdominal adipose tissues of omentum were collected and divided into control, obesity and T2DM groups. The status of APN promoter methylation was detected by denaturing high performance liquid chromatography (DHPLC), while the mRNA expression level of APN was detected by RT-PCR. Results show that methylation positive rate of APN was at the lowest level in control, middel in obesity and highest in T2DM groups, and the differences are statistically significant. Comparing the APN mRNA relative copy number of adipose tissue in each group, we found that the relative copy number of APN in control group is significantly higher than that of obesity and T2DM groups. There is a negative correlation between the mRNA expression level of APN in abdominal adipose tissue and fasting plasma glucose (FPG), glycosylated hemoglobin (HbA₁c) and triglyceride (TG) level. There is a negative correlation in DNA promoter methylation and mRNA expression of APN gene. Relative copy number of APN in DNA methylation positive group is significantly lower than that of the negative group. In conclusion, increased APN promoter methylation results in decreased mRNA expression, which induces glucose and lipid metabolic disorder, thus contributing to the initiation and development of T2DM in Xinjiang Uygur population.

Key words: Uygur people, T2DM, abdominal adipose tissue, APN gene, DNA methylation

Jun Zhang, Wangqiang Zhang, Yulei DING, Peng Xu, Tingting WANG, Wenjing Xu, Huan Lu, Zongzhi Liu, Jianxin Xie. Correlation between type 2 diabetes and DNA methylation and mRNA expression of APN in abdominal adipose tissues in Xinjiang Uygur population[J]. HEREDITAS(Beijing), 2015, 37(3): 269-275.

References

[1] Maier S, Olek A. Diabetes: a candidate disease for efficient DNA methylation profiling. J Nutr , 2002, 132(8): 2440S-2443S.
[2] Barrès R, Osler ME, Yan J, Rune A, Fritz T, Caidahl K, Krook A, Zierath JR. Non-CpG methylation of the PGC-lα promoter through DNMT3B controls mitochondrial density. Cell Metab , 2009, 10(3): 189-198.
[3] 霍丽梅, 宋光耀, 叶蔚. 脂联素与2型糖尿病发生发展的研究进展. 临床汇萃, 2005, 20(1): 54-56.
[4] 徐忠星, 张和占, 买买提·西日甫, 买买提阿不拉艾山, 库热西江·托乎提, 程钦, 董宇莉, 依米提, 艾山江. 新疆墨玉县维吾尔族糖尿病患病率调查. 中国基层医药, 2002, 9(3): 221.
[5] Scherer PE, Williams S, Fogliano M, Baldini G, Lodish HF. A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem , 1995, 270(45): 26746-26749.
[6] Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, Hara K, Mori Y, Ide T, Murakami K, Tsuboyama-Kasaoka N, Ezaki O, Akanuma Y, Gavrilova O, Vinson C, Reitman ML, Kagechika H, Shudo K, Yoda M, Nakano Y, Tobe K, Nagai R, Kimura S, Tomita M, Froguel P, Kadowaki T. The fat-derived hormonead-iponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med , 2001, 7(8): 941-946.
[7] Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, Okamoto Y, Iwahashi H, Kuriyama H, Ouchi N, Maeda K, Nishida M, Kihara S, Sakai N, Nakajima T, Hasegawa K, Muraguchi M, Ohmoto Y, Nakamura T, Yamashita S, Hanafusa T, Matsuzawa Y. Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Bio , 2000, 20(6): 1595-1599.
[8] Abbasi F, Chu JW, Lamendola C, McLaughlin T, Hayden J, Reaven GM, Reaven PD. Discrimination between obesity and insulin resistance in the relationship with adiponectin. Diabetes , 2004, 53(3): 585-590.
[9] Côté M, Mauriège P, Bergeron J, Alméras N, Tremblay A, Lemieux I, Després JP. Adiponectinemia in visceral obesity: impact on glucose tolerance and plasma lipo protein and lipid levels in men. J Clin Endocrinol Metab , 2005, 90(3): 1434-1439.
[10] Lindsay RS, Funahashi T, Hanson RL, Matsuzawa Y, Tanaka S, Tataranni PA, Knowler WC, Krakoff J. Adiponectin and development of type 2 diabetes in the pima Indian population. Lancet , 2002, 360(9326): 57-58.
[11] Bobbert T, Rochlitz H, Wegewitz U, Akpulat S, Mai K, Weickert MO, Möhlig M, Pfeiffer AF, Spranger J. Changes of adiponectin oligomer composition by moderate weight reduction. Diabetes , 2005, 54(9): 2712-2719.
[12] Maeda N, Shimomura I, Kishida K, Nishizawa H, Matsuda M, Nagaretani H, Furuyama N, Kondo H, Takahashi M, Arita Y, Komuro R, Ouchi N, Kihara S, Tochino Y, Okutomi K, Horie M, Takeda S, Aoyama T, Funahashi T, Matsuzawa Y. Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med , 2002, 8(7): 731-737.
[13] Kubota N, Terauchi Y, Yamauchi T, Kubota T, Moroi M, Matsui J, Eto K, Yamashita T, Kamon J, Satoh H, Yano W, Froguel P, Nagai R, Kimura S, Kadowaki T, Noda T. Disruption of adiponectin causes insulin resistance and neointimal formation. J Biol Chem , 2002, 277(29): 25863- 25866.
[14] Hotta K, Funahashi T, Bodkin NL, Ortmeyer HK, Arita Y, Hansen BC, Matsuzawa Y. Circulating concentrations of the adipocyte protein adiponectin are decreased in parallel with reduced insulin sensitivity during the progression to type 2 diabetes in rhesus monkey. Diabetes , 2001, 50(5): 1126-1133.
[15] Dwivedi RS, Herman JG, McCaffrey TA, Raj DS. Beyond genetics: epigenetic code in chronic kidney disease. Kidney Int , 2011, 79(1): 23-32.
[16] Bechtel W, McGoohan S, Zeisberg EM, Müller GA, Kalbacher H, Salant DJ, Müller CA, Kalluri R, Zeisberg M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med , 2010, 16(5): 544-550.
[17] Laborie LB, Mackay DJG, Temple IK, Molven A, Søvik O, Njølstad PR. DNA hypomethylation, transient neonatal diabetes, and prune

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Correlation between type 2 diabetes and DNA methylation and mRNA expression of APN in abdominal adipose tissues in Xinjiang Uygur population

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Mengxuan Xu, Ming Zhou. Advances of RNA polymerase IV in controlling DNA methylation and development in plants [J]. Hereditas(Beijing), 2022, 44(7): 567-580.
[2]	Wang Ya'nan, Tao Xu, Wanpeng Wang, Qingzhu Zhang, Xie Li'nan. Role of epigenetic modifications in the development of crops essential traits [J]. Hereditas(Beijing), 2021, 43(9): 858-879.
[3]	Xiangqian Zhang, Nan Li, Xinming Xie. Design and exploration of epigenetic comprehensive experiments [J]. Hereditas(Beijing), 2021, 43(12): 1179-1187.
[4]	Xinyue Wang, Liang Li, Qiuhui Duan, Dali Li, Jinlian Chen. Effect of Uhrf1 on intestinal development [J]. Hereditas(Beijing), 2021, 43(1): 84-93.
[5]	Hengzhen Cui, Mizhu Sun, Runzhi Wang, Chenyu Li, Yuxuan Huang, Qiuju Huang, Xiaomeng Qiao. DNA methylation in the medial prefrontal cortex regulates alcohol-related behavior in rats [J]. Hereditas(Beijing), 2020, 42(1): 112-125.
[6]	Xinyuan Wang, Yu Zhang, Nan Yang, He Cheng, Yujie Sun. DNMT3a mediates paclitaxel-induced abnormal expression of LINE-1 by increasing the intragenic methylation [J]. Hereditas(Beijing), 2020, 42(1): 100-111.
[7]	Xin Huang,Yongqiang Chen,Guoliang Xu,Shuhong Peng. DNA methylation in adipose tissue and the development of diabetes and obesity [J]. Hereditas(Beijing), 2019, 41(2): 98-110.
[8]	Xuqiong Yang, Zhenfang Wu, Zicong Li. Advances in epigenetic reprogramming of somatic cells nuclear transfer in mammals [J]. Hereditas(Beijing), 2019, 41(12): 1099-1109.
[9]	Yunfeng Pan, Yanyi Wang, Jingwen Chen, Yimei Fan. Mitochondrial metabolism’s effect on epigenetic change and aging [J]. Hereditas(Beijing), 2019, 41(10): 893-904.
[10]	Junyi Ju,Quan Zhao. Regulation of γ-globin gene expression and its clinical applications [J]. Hereditas(Beijing), 2018, 40(6): 429-444.
[11]	Chendong Liu, Lu Yang, Hongzhou Pu, Qiong Yang, Wenyao Huang, Xue Zhao, Li Zhu, Shunhua Zhang. Epigenetics regulates gene expression patterns of skeletal muscle induced by physical exercise [J]. Hereditas(Beijing), 2017, 39(10): 888-896.
[12]	Ke Zhang, Guangde Feng, Baoyun Zhang, Wei Xiang, Long Chen, Fang Yang, Mingxing Chu, Pingqing Wang. Application of epigenetic markers in molecular breeding of the swine [J]. Hereditas(Beijing), 2016, 38(7): 634-643.
[13]	Yiran Zhu, Meiling Zhang, Zhichao Zhai, Yunjiao Zhao, Xin Ma. Epigenetic regulation of genomic imprinting in germline cells and preimplantation embryos [J]. HEREDITAS(Beijing), 2016, 38(2): 103-108.
[14]	Shuli Liu, Shengli Zhang, Ying Yu. Research progress of regulatory mechanism of DNA methylation in complex traits using monozygotic twins [J]. Hereditas(Beijing), 2016, 38(12): 1043-1055.
[15]	Yangyang Liu, Hengmi Cui. The method of estimating bisulfite conversion rate in DNA methylation analysis [J]. HEREDITAS(Beijing), 2015, 37(9): 939-944.