类伸展蛋白OsPEX1对水稻花粉育性的影响

doi:10.16288/j.yczz.20-296

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Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (3): 271-279.doi: 10.16288/j.yczz.20-296

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Effect of extensin-like OsPEX1 on pollen fertility in rice

Hang Dai¹(), Yan Li¹, Shunchun Liu¹, Lei Lin¹, Juanyan Wu¹, Zhijie Zhang¹, Qichun Peng¹, Nan Li¹, Xiangqian Zhang¹()

¹ College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China

Received:2020-09-17 Online:2021-03-16 Published:2021-03-01
Supported by:
the National Natural Science Foundation of China(31671594)

Abstract

Abstract:

LRXs (leucine-rich repeat extensins) are chimeric cell wall proteins containing an N-terminal leucine-rich repeat (LRR) and a C-terminal extensin domain. Increasing evidences suggest that LRXs family genes play important roles in pollen germination and pollen tube growth in Arabidopsis thaliana. However, the functions of rice (Oryza sativa L.) LRX genes in pollen development remain poorly understood. Bioinformatics analysis showed that the rice LRX gene family consist of eight members, namely OsPEX3, OsLRX3 and OsLRX5 located on chromosome 1, OsLRX1, OsLRX3, OsLRX2,OsPEX1 and OsPEX2 located on chromosome 2, 5, 6, 11 and 12, respectively. The OsPEX1 gene is preferentially expressed in rice anther, suggesting that it may be involved in the regulation of pollen development. Next, we further investigated the role of the OsPEX1 gene in rice by knockdown of its expression using an RNAi approach. The OsPEX1 RNAi transgenic lines showed a significant decrease in seed setting rate (10%~30%) due to pollen sterility. Further quantitative RT-PCR analysis indicated that the OsPEX1 gene was significantly down-regulated in the RNAi transgenic lines. The results indicate that the OsPEX1plays an important role in the regulation of rice pollen development. Further studies on this gene could provide insights on the molecular and genetic mechanisms in this developmental process.

Key words: rice, leucine-rich repeats extensins, pollen fertility

Hang Dai, Yan Li, Shunchun Liu, Lei Lin, Juanyan Wu, Zhijie Zhang, Qichun Peng, Nan Li, Xiangqian Zhang. Effect of extensin-like OsPEX1 on pollen fertility in rice[J]. Hereditas(Beijing), 2021, 43(3): 271-279.

Figures/Tables 6

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Effect of extensin-like OsPEX1 on pollen fertility in rice

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