中国西南地区3个隔离人群遗传亚结构分析

doi:10.16288/j.yczz.22-013

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Hereditas(Beijing) ›› 2022, Vol. 44 ›› Issue (5): 424-431.doi: 10.16288/j.yczz.22-013

• Research Article • Previous Articles Next Articles

Genetic substructure analysis of three isolated populations in southwest China

Fei Wang¹(), Meng Wang¹, Xinghua Zhang², Keli Yu², Lianbin Zheng², Yajun Yang¹()

1. School of Life Sciences, Fudan University, Shanghai 200438, China
2. School of Life Sciences, Tianjin Normal University, Tianjin 300387, China

Received:2022-01-13 Revised:2022-03-20 Online:2022-05-20 Published:2022-03-29
Contact: Yang Yajun E-mail:19210700153@fudan.edu.cn;yyj229@263.net
Supported by:
Supported by the Special for basic work of science and technology No(2015FY111700);the National Natural Science Foundation of China Nos(31271283);the National Natural Science Foundation of China Nos(31401022);the National Key Research and Development Program of the Ministry of Science and Technology No(2016YFF0202301)

Abstract

Abstract:

Southwest China is home to numerous ethnic minorities, as well as many geographically and genetically isolated groups. However, the genetic substructure of these ethnic groups, especially the paternal genetic structure between groups, has not been comprehensively analyzed. In this study, we used Y chromosome capture and Illumina sequencing technologies to investigate the paternal genetic structure of three isolated groups of male unrelated individuals, including Baima in Pingwu, Sichuan Province, Muya in Shimian, Sichuan Province, and Kongge in Jinghong, Yunnan Province. We calculated the frequencies of related haplogroups by the fixed-point compound amplification method and direct counting method, and used the Past3.0 software to perform principal component analysis to draw a population clustering tree. we observed that Kongge had 3 Y chromosome haplogroups, Baima had 4 Y chromosome haplogroups, and Muya had 5 Y chromosome haplogroups. The results showed that Kongge was most closely related to the Wa, and the Y chromosome types of the Baima and Muya were mainly concentrated in the D haplogroup and its lower reaches. It has the closest relationship with the Tibetans in Qamdo and Nyingchi. The study on the genetic structure of different ethnic groups has enriched the genetic relationship of isolated populations and provided a new perspective for understanding Chinese ethnic groups.

Key words: Y chromosome, population genetics, isolated populations, genetic sub-structure

Fei Wang, Meng Wang, Xinghua Zhang, Keli Yu, Lianbin Zheng, Yajun Yang. Genetic substructure analysis of three isolated populations in southwest China[J]. Hereditas(Beijing), 2022, 44(5): 424-431.

Figures/Tables 7

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References 24

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序号	族群	语系	样本量	白马人、木雅人与参考族群的单倍群频率(%)
序号	族群	语系	样本量	DE-YAP	D1*-M174	D1a1a-M15	D1a1b1-P47	E-M40
1	壮族	仡台	181	5.51	0.86	4.65
2	傣族	仡台	176	4.55	1.7	2.27	0.57
3	侗族	仡台	147	3.4		3.4
4	黎族	仡台	55	5.45		5.45
5	水族	仡台	95	5.26		5.26
6	瑶族	苗瑶	713	13.16		13.16
7	日喀则藏族	汉藏	286	39.2	1.7	18.5	18.5	0.3
8	拉萨藏族	汉藏	219	48.4	3.7	13.2	31.1
9	山南藏族	汉藏	285	37.2	2.5	11.9	22.5
10	甘南藏族	汉藏	301	52.5	1.3	8.6	40.2
11	青海藏族	汉藏	166	48.2	1.8	7.2	36.1
12	那曲藏族	汉藏	230	54.8	3.9	12.6	37.0
13	阿里藏族	汉藏	24	29.2		20.8	8.4
14	云南藏族	汉藏	29	37.9		6.9	31.0
15	昌都藏族	汉藏	278	62.2	0.4	9.0	49.3
16	林芝藏族	汉藏	144	68.1	4.9	16.7	44.4
17	嘉绒藏族	汉藏	92	46.7	1.1	30.4	9.8
18	川西藏族	汉藏	300	57.3	0.3	16.0	34.3	1.3
18	木雅	汉藏	74	59.5		8.1	51.4
20	白马	汉藏	167	61.1	1.2	41.9	18.0
21	羌族	汉藏	152	61.8	2.0	54.6	5.3
22	彝族	汉藏	107	14.2	0.9	13.1
23	拉祜族	汉藏	58	26.7		26.7
24	基诺族	汉藏	93	3.5		3.5

序号	族群	语系	样本量	空格人与参考族群的单倍群频率(%)
序号	族群	语系	样本量	D	C-M217	F2	N	O	Q
1	佤族	南亚	16	0	6.25	0	31.25	62.5	0
2	广西侗族	仡台	17	0	5.882353	0	0	94.11765	0
3	黎族	仡台	14	7.142857	0	0	14.28571	78.57143	0
4	毛南族	仡台	15	6.666667	0	0	0	93.33333	0
5	仡佬族	仡台	21	0	4.761905	0	14.28571	80.95238	0
6	湖南侗族	仡台	45	2.222222	22.22222	0	6.666667	66.66667	2.222222
7	苗族	苗瑶	20	0	0	0	0	100	0
8	白族	汉藏	34	5.882353	2.941176	0	8.823529	82.35294	0
9	哈尼族	汉藏	60	1.666667	21.66667	3.333333	15	55	3.333333
10	景颇族	汉藏	30	0	0	0	10	90	0
11	纳西族	汉藏	12	0	8.333333	0	41.66667	50	0
12	土家族	汉藏	33	3.030303	21.21212	0	6.060606	66.66667	3.030303
13	羌族	汉藏	27	33.33333	0	0	33.33333	29.62963	0
14	彝族	汉藏	24	16.66667	12.5	0	4.166667	66.666	0
15	空格	南亚	48	0	10.41667	0	54.16667	35.41667333	0

Genetic substructure analysis of three isolated populations in southwest China

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