沼泽型水牛Y染色体微卫星标记的筛选与多态性检测

doi:10.3724/SP.J.1005.2010.00242

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (3): 242-247.doi: 10.3724/SP.J.1005.2010.00242

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Screening and polymorphism of Y chromosome microsatellite markers in swamp buffalo

ZHANG Xiao-Ming, YUE Xiang-Peng, ZHANG Chun-Mei, LAN Xian-Yong, CHEN Hong, LEI Chu-Zhao

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received:2009-08-31 Revised:2009-12-03 Online:2010-03-20 Published:2010-03-15
Contact: LEI Chu-Zhao E-mail:leichuzhao1118@126.com

Abstract

Abstract:

To understand the genetic diversity of buffalo Y chromosome, 14 microsatellite markers specific for cattle Y chromosomes were used to analyze 31 male buffaloes of three populations (i.e., HH, BN, and PR) originating from southeastern Yunnan Province, P. R. China. The aim of this study was to examine the practicability of cattle Y chromosome-specific microsatellite markers in studying genetic diversity of buffalo Y chromosome. Three markers (INRA008, UMN0103, and UMN0504) produced single allele, and three (UMN1113, UMN0304, and BC1.2) produced three alleles. But these markers were all monomorphic. Another three markers (UMN0920, UMN0307, and UMN3008) amplified irregular ladder-like bands. These markers were not suitable for investigating the swamp buffalo Y chromosome genetic diversity. The remaining five microsatellites (INRA124, INRA189, BM861, PBR1F1, and UMN2001) were polymorphic, which can be used to study the swamp buffalo Y chromosome genetic diversity. The mean number of alleles (Na), expected heterozygosity (He), genetic diversity (GD), polymorphism information content (PIC), and Shannon's information index (SI) across these five polymorphic loci in the buffalo population samples studied were 2.8000, 0.3998, 0.4144, 0.3245, and 0.5849, respectively, demonstrating a moderate degree of polymorphism in the Y chromosome of the buffalo population.

Key words: swamp buffalo, Y chromosome microsatellite, genetic diversity

ZHANG Xiao-Meng, LE Xiang-Feng, ZHANG Chun-Mei, LA Xian-Yong, CHEN Hong, LEI Chu-Chao. Screening and polymorphism of Y chromosome microsatellite markers in swamp buffalo[J]. HEREDITAS, 2010, 32(3): 242-247.

References

[1] FAO《生产年鉴2007》. Http://www.fao.org/.

[2] Lei CZ, Zhang W, Chen H, Lu F, Ge QL, Liu RY, Dang RH, Yao YY, Yao LB, Lu ZF, Zhao ZL. Two maternal lineages revealed by mtDNA D-loop sequences in Chinese native water buffaloes (Bubalus bubalis). Asian-Austral J Anim Sci, 2007, 20(4): 471–476.

[3] Lei CZ, Zhang W, Chen H, Lu F, Liu RY, Yang XY, Zhang HC, Liu ZG, Yao LB, Lu ZF, Zhao ZL. Independent ma-ternal origin of Chinese swamp buffalo (Bubalus bubalis). Anim Genet, 2007, 38(2): 97–102.

[4] 张毅. 中国水牛分子遗传多样性及其起源分化的研究[学位论文]. 中国农业大学, 2006.

[5] Hoffmann I, Marsan PA, Barker JSF, Cothran EG, Hanotte O, Lenstra JA, Milan D, Weigend S, Simianer H. New MoDAD marker sets to be used in diversity studies for the major farm animal species: recommendations of a joint ISAG/FAO working group. In: Proc. 29th International Conference on Animal Genetics. Tokyo, Japan, 2004.

[6] Liu WS, Mariani P, Beattie CW, Alexander LJ, Ponce de Leon FA. A radiation hybrid map for the bovine Y Chro-mosome. Mamm Genome, 2002, 13(6): 320–326.

[7] Liu WS, Beattie CW, Ponce de Leon FA. Bovine Y chro-mosome microsatellite polymorphisms. Cytogenet Ge-nome Res, 2003, 102 (1–4): 53–58.

[8] Raymond M, Rousset F. GENEPOP (version 1.2): popula-tion genetic software for exact tests and ecumenicism. J Hered, 1995, 86(3): 248–249.

[9] Goudet J. 2001. FSTT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). available from http://www.unil.ch/izea/softwares/fstat.htmi.

[10] Marshall TC, Slate J, Kruuk LEB, Pemberton JM. Statis-tical confidence for likelihood-based paternity inference in natural populations. Mol Ecol, 1998, 7(5): 639–655.

[11] Edwards CJ, Gaillard C, Bradley DG, MacHugh DE. Y-specific microsatellite polymorphisms in a range of bo-vid species. Anim Genet, 2000, 31(2): 127–130.

[12] Li MH, Zerabruk M, Vangen O, Olsaker I, Kantannen. Re-duced genetic structure of north Ethiopian cattle revealed by Y-chromosome analysis. Heredity, 2007, 98 (4): 214–221.

[13] Van Hooft WF, Groen AF, Prins HT. Phylogeography of the African buffalo based on mitochondrial and Y-chromosomal loci: Pleistocene origin and population expansion of the Cape buffalo subspecies. Mol Ecol, 2002, 11(2): 267–279.

[14] Van Hooft P, Greyling BJ, Prins HHT, Getz WM, Jolles AF. Selection at the Y chromosome of the African buffalo driven by rainfall. 2007, 2(10): e1086–e1090.

[15] Schwerin M, Gallagher DS, Miller JR, Thomsen PD. Mapping of repetitive bovine DNA sequences on cattle Y chromosome. Cytogenet Cell Genet, 1992, 61(3): 189–194.

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Screening and polymorphism of Y chromosome microsatellite markers in swamp buffalo

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