大黄鱼耐低温性状相关微卫星标记的筛选

doi:10.3724/SP.J.1005.2010.00248

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (3): 248-253.doi: 10.3724/SP.J.1005.2010.00248

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Screening of microsatellite markers associated with cold tolerance of large yellow croaker (Pseudosciaena crocea R.)

GAO Guo-Qiang^{1, 2}, CHANG Yu-Mei¹, HAN Qi-Xia^{1, 2}, CHI Bing-Jie^{1, 2}, LI Ming-Yun³, XUE Liang-Yi³,
LIANG Li-Qun¹

1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
3. College of Life Sciences, Ningbo University, Ningbo 315211, China

Received:2009-07-09 Revised:2009-10-27 Online:2010-03-20 Published:2010-03-15
Contact: LIANG Li-Qun E-mail:llq-1019@163.com

Abstract

Abstract:

Cold tolerance is one of the major economic characters in fish. In order to discuss the cold tolerance of large yellow croaker (Pseudosciaena crocea R.), fifteen fluorescent dye-labeled microsatellite markers were applied to detect genetic differences between F₁ offsprings of cold tolerance group and normal group of large yellow croaker by SSR-PCR. Each group contained 20 randomly and separately sampled individuals. As a result, marker LYC0002 five alleles (LYC0002_{104 bp}, LYC0002_{106 bp}, LYC0002_{108 bp}, LYC0002_{110 bp}, and LYC0002_{112 bp}) were amplified with marker LYC0002 in both groups and 60% (12/20) of individuals had allele LYC0002_{112 bp} in cold tolerance group exclusively, which indicated that this allele is probably sensitive to temperature and associated with gene for cold tolerance. In addition, four alleles (LYC0002_{106 bp}, LYC0002_{108 bp}, LYC0002_{110 bp}, and LYC0002_{112 bp}) were sequenced individually. Sequence alignments showed that LYC0002_{112 bp} allele contains 10 (CA) repeats, the remaining three alleles lacked one (CA) one by one, corresponding to the stepwise mutation model (SMM) of microsatellite.

Key words: large yellow croaker (Pseudosciaena crocea R.), cold tolerance, microsatellite, fluorescent dye

GAO Guo-Jiang, CHANG Yu-Mei, HAN Qi-Xia, CHE Bing-Jie, LI Meng-Yun, XUE Liang-Xi, LIANG Li-Qun. Screening of microsatellite markers associated with cold tolerance of large yellow croaker (Pseudosciaena crocea R.)[J]. HEREDITAS, 2010, 32(3): 248-253.

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Screening of microsatellite markers associated with cold tolerance of large yellow croaker (Pseudosciaena crocea R.)

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