细胞周期相关蛋白cyclin D1、p53和p21在食管鳞癌中的表达改变及其病理学意义

doi:10.3724/SP.J.1005.2010.00455

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (5): 455-460.doi: 10.3724/SP.J.1005.2010.00455

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Expression of cell cycle related proteins cyclin D1, p53 and p21 in esophageal squamous cell carcinoma

LIN De-Chen¹, SHI Zhi-Zhou¹, XUE Li-Yan², CHEN Wei¹, XU Xin¹, HAN Ya-Ling¹, LV Ning², WANG Ming-Rong¹

1. State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China;
2. Department of Pathology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China

Received:2010-01-15 Revised:2010-03-14 Online:2010-05-20 Published:2010-05-15
Contact: Mingrong Wang E-mail:wangmr04@126.com

Abstract

Abstract:

Dysregulation of cell cycle control, especially G₁/S phase transition, is implicated in the pathogenesis of most human cancers, including esophageal squamous cell carcinoma (ESCC). However, the clinicopathological significance of aberrant expression of G₁/S phase-related proteins in ESCC remains unclear. In the present study, cyclin D1, p21^WAF1/Cip1and p53 protein expression were examined in 148 ESCC cases using immunohistochemistry combined with tissue microarray. We then analyzed the correlations between their expression and clinicopathological parameters and found that overexpression of p53 was associated with lymph node metastasis (P = 0.001). p21^WAF1/Cip1down-regulation was an independent prognostic factor by multivariate analysis (RR = 0.418, P <0 .001). Further more, array-CGH results revealed that cyclin D1 gene was amplified in 45.4% of ESCC patients. This study confirms that dysregulation of G1/S related genes is common in ESCC and reduced expression of p21^WAF1/Cip1 protein can predict shorter overall survival time of patients with ESCC.

Key words: esophageal squamous cell carcinoma, cell cycle regulation, immunohistochemistry, tissue microarray, prognosis

LIN De-Chen, SHI Zhi-Zhou, XUE Li-Yan, CHEN Wei, XU Cuan, HAN E-Ling, LV Ning, WANG Meng-Rong. Expression of cell cycle related proteins cyclin D1, p53 and p21 in esophageal squamous cell carcinoma[J]. HEREDITAS, 2010, 32(5): 455-460.

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Expression of cell cycle related proteins cyclin D1, p53 and p21 in esophageal squamous cell carcinoma

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