微卫星标记分析罗非鱼群体的遗传潜力

doi:10.3724/SP.J.1005.2011.00768

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (7): 768-775.doi: 10.3724/SP.J.1005.2011.00768

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Genetic potential analysis of six Tilapia populations by microsatellite DNA markers

YANG Hong¹, LI Da-Yu¹, CAO Xiang^{1, 2}, ZOU Zhi-Ying¹, XIAO Wei¹, ZHU Jing-Lin¹

1. Key Laboratory of Genetic Breeding and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China 2. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China

Received:2010-08-31 Revised:2011-01-18 Online:2011-07-20 Published:2011-07-25

Abstract

Abstract: Twenty five microsatellite loci were used to analyze two blue tilapia populations [“Xia’ao 1” (ZA), Guangxi population] and four nile tilapia populations [Egypt strain (ZN), 88 strain (XN), Guangxi population (GN), American strain (MN)]. A total of 7775 fragments ranging from 100 bp to 400 bp in length were obtained. Three to eight alleles were amplified in 25 loci and 143 alleles in all the six populations. The average number of alleles in each locus was 5.72. The average values of observed heterozygosity (Ho) ranged from 0.7253 to 0.8160, the average expected heterozygosity (He) ranged from 0.5146 to 0.6834, the average polymorphic information content (PIC) ranged from 0.4212 to 0.6105, and the number of average effective alleles (Ae) ranged from 2.20 to 3.23. The highest genetic similarity index was 0.9130 (between ZA and GA); and the lowest was 0.4352 (between ZA and ZN). The results showed that the four nile tilapia populations contained a high level of genetic potential, and the two blue tilapia populations were moderate.

Key words: genetic potential, O. Aureus, O. niloticus, microsatellite, genetic diversity

YANG Hong, LI Da-Yu, CAO Xiang, JU Zhi-Yang, XIAO Wei, CHU Jing-Lin. Genetic potential analysis of six Tilapia populations by microsatellite DNA markers[J]. HEREDITAS, 2011, 33(7): 768-775.

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Genetic potential analysis of six Tilapia populations by microsatellite DNA markers

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