马铃薯抗晚疫病基因Rpi-blb2的LRR区域多态性及分子进化分析

doi:10.3724/SP.J.1005.2012.00485

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HEREDITAS ›› 2012, Vol. 34 ›› Issue (4): 485-494.doi: 10.3724/SP.J.1005.2012.00485

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Nucleotide polymorphism and molecular evolution of the LRR region in potato late blight resistance gene Rpi-blb2

YOU Lu-Peng, MIAO Jing, ZOU Ai-Lan, QI Jin-Liang, YANG Yong-Hua

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China

Received:2011-07-03 Revised:2012-01-08 Online:2012-04-20 Published:2012-04-25

Abstract

Abstract: Rpi-blb2, which is originally derived from Solanum bulbocastanum, is a broad-spectrum potato late blight resistance gene and belongs to the NBS-LRR family. Here, the LRR homologues of Rpi-blb2 were cloned with PCR method from 40 potato cultivars (including 20 resistant potato cultivars and 20 susceptible ones) and 7 wild potato popula-tions. Then, the similarities of the sequences, polymorphic (segregating) sites, and nucleotide diversities were estimated by bioinformatic methods. The results showed that high nucleotide polymorphism and some hot-spot mutations existed in the LRR region of Rpi-blb2. The test of Ka/Ks ratio showed that the function of LRR was conserved because of the purifying selection, although different positions of the Rpi-blb2 LRR region were under different selection pressures. Moreover, the LRR region of Rpi-blb2 had no clear differentiation between the cultivated and wild potatoes.

Key words: potato late blight, Rpi-blb2, LRR region, polymorphism

CLC Number:

YOU Lu-Peng, MIAO Jing, ZOU Ai-Lan, QI Jin-Liang, YANG Yong-Hua. Nucleotide polymorphism and molecular evolution of the LRR region in potato late blight resistance gene Rpi-blb2[J]. HEREDITAS, 2012, 34(4): 485-494.

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Nucleotide polymorphism and molecular evolution of the LRR region in potato late blight resistance gene Rpi-blb2

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