大豆C2H2型锌指蛋白基因SCTF-1的克隆及功能分析

doi:10.3724/SP.J.1005.2012.00749

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HEREDITAS(Beijing) ›› 2012, Vol. 34 ›› Issue (6): 749-756.doi: 10.3724/SP.J.1005.2012.00749

Cloning and functional analysis of SCTF-1 encoding a C₂H₂-type zinc finger protein from soybean

SONG Bing^1,2, WANG Pi-Wu¹, FU Yong-Ping¹, FAN Xu-Hong³, XIA Hai-Feng^1,4, GAO-Wei⁴, HONG Yang¹, WANG He¹, ZHANG Zhuo¹, MA Jian¹

1. College of Agriculture, Jilin Agricultural University, Changchun 130118, China 2. Jilin City Academy of Agricultural Sciences, Jilin City 132101, China 3. Soybean Research Center, Jilin Academy of Agricultural Sciences, Changchun 130033, China 4. Tonghua Academy of Agricultural Sciences, Tonghua 135000, China

Received:2012-02-09 Revised:2012-03-02 Online:2012-06-20 Published:2012-06-25

Abstract

Abstract:

The zinc finger protein is one of the proteins with finger-like domain. Some of them are transcription factors which play important role in plant growth and plant resistance to abiotic stresses. In this paper, a novel C₂H₂-type zinc finger protein gene SCTF-1 (GenBank accession number JQ692081) was isolated from soybean (Glycine max (L.) Merr.) This gene has a 699 bp ORF (open reading frame) with no in-tron and encodes a 24.9 kDa protein with 233 amino acids. Its isoelectric point (pI) is 8.33. The SCTF-1 protein contains two typical C₂H₂-type zinc finger domains. Both of them have highly conserved amino acid sequence-QALGGH which is a particular characteristic of plant. Transient expression of the GFP-SCTF-1 protein in onion epidermal cell showed that SCTF-1 was localized in cell nuclei. RT-PCR results showed that SCTF-1 gene was expressed with high levels in flowers and leaves in soybean, but low in roots and stems. The expression of SCTF-1 gene was strongly induced by low temperature in the soybean seedlings. Overexpression of SCTF-1 enhanced cold tolerance of transgenic tobacco (Nicotiana tabacum L.) compared to the control.

Key words: soybean, zinc finger protein, C₂H₂-type, SCTF-1 gene, subcellular localization, cold tolerance

SONG Bing, WANG Pi-Wu, FU Yong-Ping, FAN Xu-Hong, XIA Hai-Feng, GAO-Wei, HONG Yang, WANG He, ZHANG Zhuo, MA Jian. Cloning and functional analysis of SCTF-1 encoding a C₂H₂-type zinc finger protein from soybean[J]. HEREDITAS(Beijing), 2012, 34(6): 749-756.

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Cloning and functional analysis of SCTF-1 encoding a C₂H₂-type zinc finger protein from soybean

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Cloning and functional analysis of SCTF-1 encoding a C2H2-type zinc finger protein from soybean

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Cloning and functional analysis of SCTF-1 encoding a C₂H₂-type zinc finger protein from soybean