TNF-a基因多态性及其与奶牛乳房炎的相关性分析

doi:10.3724/SP.J.1005.2010.00929

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (9): 929-934.doi: 10.3724/SP.J.1005.2010.00929

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Polymorphism of bovine TNF-a gene and its association with mastitis in Chinese Holstein cows

XU A-Juan, LIU Xiao-Lin, GUO Jia-Zhong, XIA Zhi

Shaanxi Key Laboratory of Molecular Biology for Agriculture, Animal Science Technology College of Northwest A & F University, Yan-gling 712100, China

Received:2009-12-24 Revised:2010-03-23 Online:2010-09-20 Published:2010-09-20
Contact: LIU Xiao-Lin E-mail:liuxiaolin@nwsuaf.edu.cn

Abstract

Abstract: Four hundred and seven Chinese Holstein cows were chosen and divided into 2 groups (healthy group (317), sub-clinical and clinical group (100)) to investigate variation in bovine Tumor necrosis factor-alpha (TNF-a) gene and analyze their associations with mastitis. By PCR-RFLP and CRS-RFLP analyses, three polymorphic sites were detected, with one G→A mutation at 39 bp in exon2 and one C→T mutation at 293 bp in exon4, and C→G mutation in 5´UTR. The three polymorphic sites were able to be cut by DraⅠ, AfaⅠ, and DdeⅠ restriction enzymes respectively, and the DraⅠlocus was Create Restriction Enzyme Cutting Site. Chi-square analysis suggested that the three polymorphic sites did not reach Hardy-Weinberg equilibrium (P<0.05). Least square linear model (LSM) analysis indicated that the DraⅠ, AfaⅠ, and DdeⅠ loci of TNF-a gene were associated with somatic cell score (SCS) (P<0.05). Genotype AA in DraⅠlocus and BB in AfaⅠ locus showed significantly higher SCS (P<0.05) and genotype AB in DdeⅠlocus showed significantly lower SCS than AA and BB genotypes in the population tested (P<0.05). This suggested that genotypes BB in DraⅠloci, AA in AfaⅠloci, and AB in DdeⅠloci can be used as candidate markers for mastitis resistance selection in dairy cattle.

Key words: TNF-a gene, PCR-RFLP, polymorphism, mastitis, relativity

XU A-Juan, LIU Xiao-Lin, GUO Jia-Zhong, JIA Zhi. Polymorphism of bovine TNF-a gene and its association with mastitis in Chinese Holstein cows[J]. HEREDITAS, 2010, 32(9): 929-934.

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Polymorphism of bovine TNF-a gene and its association with mastitis in Chinese Holstein cows

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