植物质体基因工程调控元件研究进展

doi:10.16288/j.yczz.23-021

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Hereditas(Beijing) ›› 2023, Vol. 45 ›› Issue (6): 501-513.doi: 10.16288/j.yczz.23-021

• Review • Previous Articles Next Articles

Progress on regulatory elements of plant plastid genetic engineering

Yifan Yu^1,²(), Zhen OuYang², Juan Guo¹, Yujun Zhao¹(), Luqi Huang^1,²()

1. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China

Received:2023-01-30 Revised:2023-04-29 Online:2023-06-20 Published:2023-05-22
Contact: Zhao Yujun,Huang Luqi E-mail:18641603721@163.com;zhaoyj@nrc.ac.cn;huangluqi01@126.com
Supported by:
Key Project at Central Government Level(2060302);Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ15-YQ-061);Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZXT202103)

Abstract

Abstract:

With the advancement of plant synthetic biology, plastids have emerged as an optimal platform for the heterologous production of numerous commercially valuable secondary metabolites and therapeutic proteins. In comparison on nuclear genetic engineering, plastid genetic engineering offers unique advantages in terms of efficient expression of foreign genes and biological safety. However, the constitutive expression of foreign genes in the plastid system may impede plant growth. Therefore, it is imperative to further elucidate and design regulatory elements that can achieve precise regulation of foreign genes. In this review, we summarize the progress made in developing regulatory elements for plastid genetic engineering, including operon design and optimization, multi-gene coexpression regulation strategies, and identification of new expression regulatory elements. These findings provide valuable insights for future research.

Key words: plant synthetic biology, plastid genetic engineering, genetic transformation, operon, regulatory element

Yifan Yu, Zhen OuYang, Juan Guo, Yujun Zhao, Luqi Huang. Progress on regulatory elements of plant plastid genetic engineering[J]. Hereditas(Beijing), 2023, 45(6): 501-513.

Figures/Tables 3

Fig. 1

Table 1

Table 2

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研究内容	启动子	5′UTR	3′UTR	参考文献
苦瓜(Momordica charantia)叶绿体转化载体的构建及其功能评价	PpsbA	psbA	TpsbA	[41]
可育转质体拟南芥植物的高效培育	Prrn/PpsbA/Pclp	rbcL/T7g10L	TpsbA/TatpA/Trps16/ TatpB/TrrnB	[19]
适用于拟南芥质体转化的载体及壮观霉素敏感性拟南芥品系的开发	Prrn	atpB	TpsbA	[42]
莴苣叶绿体用于生产口服增强型疫苗抗原SARS-CoV-2刺突蛋白	Prrn/PpsbA	psbA	TrbcL/TpsbA	[43]
RuBisCO的基因工程改良	PrbcL	rbcL	Trps16/TrbcL	[31]
药用植物黄花蒿叶绿体遗传转化体系的建立	Prrn/PpsbA	T7g10L/psbA	TpsbA/Trps16	[24]
揭示质体编码的乙酰辅酶A羧化酶基因对代谢和发育功能的影响	Prrn		TpsbA	[44]
将复制微型染色体开发为质体基因工程的新工具	Prrn/PclpP	T7g10L	TrrnB/TpsbA	[45]

研究内容	启动子	5′UTR	3′UTR	参考文献
烟草新型IEE的开发	Prrn	T7g10L	TpsbA/TrbcL/Trps16	[92]
莱茵衣藻新型IEE的开发	PrbcL	T7g10L	TrbcL	[38]
工程化PPR蛋白在新型质体诱导表达调控系统中的应用	Prrn/PpsbA	atpB/atpH	TpsbA/TrbcL	[71]
工程化RNA结合蛋白在新型非绿色质体诱导表达调控系统中的应用	Prrn	atpB/atpH	TpsbA/TrbcL	[99]
质体未加工多顺反子中ORF独立翻译合成系统的开发	Prrn/PpsbA	clp/rbcL/atpB/T7g10L/ psbA/cry9Aa2/atpH	TpsbA	[106]
核编码翻译增强子TDA1在莱茵衣藻叶绿体诱导表达调控系统中的应用	Prrn	atpA		[72]
核糖开关质体诱导表达系统在虾青素生物合成中的应用	T7/Prrn/PpsbA	T7g10L/psbA	TpsbA/TatpA/Trps16/ TrbcL/T7 T	[33]
莱茵衣藻新型温度敏感表达调控系统的开发	PpsaA	psaA	TrbcL	[105]
莱茵衣藻维生素可逆性调控系统的开发	PpsbD/PpsaA	psbD/psaA	TrbcL/TpsaD	[107]

Progress on regulatory elements of plant plastid genetic engineering

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