基于CRISPR/Cas9系统在全基因组范围内筛选功能基因及调控元件研究进展

doi:10.16288/j.yczz.19-390

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Hereditas(Beijing) ›› 2020, Vol. 42 ›› Issue (5): 435-443.doi: 10.16288/j.yczz.19-390

• Review • Previous Articles Next Articles

Progress on genome-wide CRISPR/Cas9 screening for functional genes and regulatory elements

Siyuan Liu^1,², Guoqiang Yi², Zhonglin Tang^2,³(), Bin Chen¹()

^1. College of Animal Science & Technology, Hunan Agricultural University, Changsha 410128, China;
^2. Genome Analysis Laboratory of the Ministry of Agriculture, Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
^3. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2020-01-02 Revised:2020-04-08 Online:2020-05-20 Published:2020-04-26
Contact: Tang Zhonglin,Chen Bin E-mail:tangzhonglin@caas.cn;chenbin7586@126.com
Supported by:
Supported by Position Expert of Hunan Province Pig Industry Technology System No(2019-2021);Shenzhen Key Technology Projects No(JSGG20180507182028625);National Science and Technology Major Project of China No(2016ZX08006002-005);the Key R&D Programmes of Guangdong Province No(2018B020203002)

Abstract

Abstract:

The CRISPR/Cas9 system is a powerful tool which has been extensively used for genome editing in the past few years. Nuclease-dead Cas9 (CRISPR/dCas9), a Cas9 protein mutant without splicing ability, along with loss-of- function (LOF), gain-of-function (GOF), or non-coding genes scanning approaches can reveal genome-scale functional determinants. CRISPR/Cas9 has been widely adopted to decipher disease mechanisms and pinpoint drug targets in the life science field, and also provide novel insights into animal genetics and breeding. In this review, we summarize the research progress in high-throughput CRISPR/Cas9 screening for revealing the functional genes and regulatory elements in the whole genome. We also highlight the applications of CRISPR/Cas9 system in the animal cells, providing a reference for gene editing and other related research in related fields.

Key words: CRISPR/Cas9, genome-wide screening, functional gene, regulatory elements

Siyuan Liu, Guoqiang Yi, Zhonglin Tang, Bin Chen. Progress on genome-wide CRISPR/Cas9 screening for functional genes and regulatory elements[J]. Hereditas(Beijing), 2020, 42(5): 435-443.

Figures/Tables 1

References 68

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Progress on genome-wide CRISPR/Cas9 screening for functional genes and regulatory elements

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