锌指蛋白核酸酶的作用原理及其应用

doi:锌指结构域; 锌指蛋白; 锌指蛋白核酸酶; 同源重组; 基因打靶

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (2): 123-130.doi: 锌指结构域; 锌指蛋白; 锌指蛋白核酸酶; 同源重组; 基因打靶

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The mechanism and application of zinc finger nucleases

ZHONG Qiang , ZHAO Shu-Hong

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2010-07-01 Revised:2010-11-07 Online:2011-02-20 Published:2011-02-25
Contact: ZHAO Shu-Hong E-mail:shzhao@mail.hzau.edu.cn

Abstract

Abstract: Due to specific recognization of DNA sequences and designability, zinc finger nucleases (ZFN) has been used in knock in and knock out genes. Because of high ratio of homologous recombination of DSB-GT induces by ZFN, ZFN technology has been considered as the most powerful tool for gene modification. It has been successfully used at cell or embryo levels in plants and animals. Under the rapid development of high affinity zinc finger protein (ZFP), this technique will be applied to genetic engineering and breeding extensively in the future. This review discussed the DNA recognization mechanism and double strand break gene targeting (DSB-GT) of zinc finger nucleases (ZFN). Some application examples of ZFN were summarized.

Key words: zinc finger domain, zinc finger protein, zinc finger nucleases, homologous recombination, gene targeting

ZHONG Jiang, DIAO Shu-Gong. The mechanism and application of zinc finger nucleases[J]. HEREDITAS, 2011, 33(2): 123-130.

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