应用MSAP方法检测鸡不同组织基因组的甲基化状态

doi:10.3724/SP.J.1005.2011.00620

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (6): 620-626.doi: 10.3724/SP.J.1005.2011.00620

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Analysis of DNA methylation in different chicken tissues with MSAP

XU Qing¹, ZHANG Yuan², SUN Dong-Xiao², WANG Ya-Chun², TANG Shao-Qing², ZHAO Meng¹

1. Institute of Life Science and Biotechnology, Beijing Jiaotong University, Beijing 100044, China 2. Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received:2010-10-14 Revised:2010-12-08 Online:2011-06-20 Published:2011-06-25
Contact: SUN Dong-Xiao E-mail:sundx@cau.edu.cn

Abstract

Abstract: With methylation sensitive amplified polymorphism (MSAP), the DNA methylation levels and patterns of CCGG sites in genomes was analyzed among four different tissues and between parents and offsprings from three groups of adult chicken, White Leghorn, White Plymouth Rock, and their F₁ hybrids. The results indicated that the degree of methylation was approximate 29.7% in muscle, 27.5% in liver, 27.5% in heart, and 26.1% in kidney. There was significantly different in the level of methylation in the 3 different groups and in 4 different tissues (P<0.05). The fully-methylated sites were less than the hemi-methylated sites among the 4 tissues, which was different from that of plants. The two tissue-specific MSAP fragments were isolated, sequenced, and characterized, both of which were located in the coding regions. These results clearly demonstrated that there was difference in the methylation level among various tissues and different groups, which suggested that the genetic factor may have effect on the individual methylation level.

Key words: chicken, genome, methylation, MSAP, group

XU Jing, ZHANG Yuan, SUN Dong-Xiao, WANG Ya-Chun, TANG Chao-Jing, DIAO Meng. Analysis of DNA methylation in different chicken tissues with MSAP[J]. HEREDITAS, 2011, 33(6): 620-626.

References

[1] Kurkjian C, Kummar S, Murgo AJ. DNA methylation: its role in cancer development and therapy. Curr Probl Cancer, 2008, 32(5): 187-235.
[2] Gopalakrishnan S, van Emburgh BO, Robertson KD. DNA methylation in development and human disease. Mutat Res, 2008, 647(1-2): 30-38.
[3] Vanyushin BF. Enzymatic DNA methylation is an epigenetic control for genetic functions of the cell. Biochemistry (Mosc), 2005, 70(5): 488-499.
[4] Bird A. DNA methylation patterns and epigenetic memory. Genes Dev, 2002, 16(1): 6-21.
[5] Mandel JL, Chambon P. DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes. Nucleic Acids Res, 1979, 7(8): 2081-2103.
[6] 唐韶青, 张沅, 徐青, 孙东晓, 俞英. 不同动物部分组织基因组甲基化程度的差异分析. 农业生物技术学报, 2006, 14(4): 507-511.
[7] 班谦, 赵宗胜, 曹体婷. 家鸡胚胎发育过程DNA甲基化的MSAP检测. 安徽农业科学, 2009, 37(21): 9902-9904.
[8] Lu YL, Rong TZ, Cao MJ. Analysis of DNA methylation in different maize tissues. J Genet Genomics, 2008, 35(1): 41-48.
[9] Xu ML, Li XQ, Korban SS. AFLP-based detection of DNA methylation. Plant Mol Biol Rep, 2000, 18(4): 361-368.
[10] McClelland M, Nelson M, Raschke E. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucleic Acids Res, 1994, 22(17): 3640-3659.
[11] Labra M, Grassi F, Imazio S, Di Fabio T, Citterio S, Sgorbati S, Agradi E. Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L. Chemosphere, 2004, 54(8): 1049-1058.
[12] Aina R, Sgorbati S, Santagostino A, Labra M, Ghiani A, Citterio S. Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp. Physiol Plant, 2004, 121(3): 472-480.
[13] Li XQ, Xu ML, Korban SS. DNA methylation profiles differ between field-and in vitro-grown leaves of apple. J Plant Physiol, 2002, 159(11): 1229-1234.
[14] de Montera B, Boulanger L, Taourit S, Renard JP, Eggen A. Genetic identity of clones and methods to explore DNA. Cloning Stem Cells, 2004, 6(2): 133-139.
[15] Xiong LZ, Xu CG, Saghai Maroof MA, Zhang QF. Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique. Mol Gen Genet, 1999, 261(3): 439-446.
[16] Shaked H, Kashkush K, Ozkan H, Feldman M, Levy AA. Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell, 2001, 13(8): 1749-1759.
[17] Gardiner-Garden M, Frommer M. CpG islands in verte-brate genomes. J Mol Biol, 1987, 196(2): 261-282.
[18] Waalwijk C, Flavell RA. DNA methylation at a CCGG sequence in the large intron of the rabbit β-globin gene: tissue-specific variations. Nucleic Acids Res, 1978, 5(12): 4631-4634.
[19] Grunau C, Hindermann W, Rosenthal A. Large-scale methylation analysis of human genomic DNA reveals tissue-specific differences between the methylation profiles of genes and pseudogenes. Hum Mol Genet, 2000, 9(18): 2651-2663.
[20] Song F, Smith JF, Kimura MT, Morrow AD, Matsuyama T, Nagase H, Held WA. Association of tissue-specific differentially methylated regions (TDMs) with differential gene expression. Proc Natl Acad Sci USA, 2005, 102(9): 3336-3341.
[21] Messeguer R, Ganal MW, Steffens JC, Tanksley SD. Characterization of the level, target sites and inheritance of cytosine methylation in tomato nuclear DNA. Plant Mol Biol, 1991, 16(5): 753-770.
[22] Holliday R, Pugh JE. DNA modification mechanisms and gene activity during development. Science, 1975, 187(4173): 226-232.
[23] Walsh CP, Bestor TH. Cytosine methylation and mammalian development. Genes Dev, 1999, 13(1): 26-34.

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Analysis of DNA methylation in different chicken tissues with MSAP

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