RAD51调控REV1参与DNA双链断裂修复

doi:10.16288/j.yczz.18-176

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Hereditas(Beijing) ›› 2018, Vol. 40 ›› Issue (11): 1007-1014.doi: 10.16288/j.yczz.18-176

• Orginal Article • Previous Articles Next Articles

RAD51 regulates REV1 recruitment to DNA double-strand
break sites

Min Huang^1,²,Yeran Yang^1,²,Xiaoyan Sun¹,Ting Zhang¹,Caixia Guo^1,^2,³()

^1. CAS Key Laboratory of Genomics and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
^2. University of Chinese Academy of Sciences, Beijing 100049, China
^3. School of Future Technology, Chinese Academy of Sciences, Beijing 101408, China

Received:2018-06-29 Revised:2018-10-19 Online:2018-11-20 Published:2018-10-30
Contact: Guo Caixia E-mail:guocx@big.ac.cn
Supported by:
Supported by the National Key Research and Development Program of China(2018YFA0108500);the National Natural Science Foundation of China(81630078);the National Natural Science Foundation of China(31471331)

Abstract

Abstract:

REV1 is one of the major Y-family DNA polymerases. It not only functions as a scaffold protein to mediate other specialized DNA polymerases to sites of lesions, but also inserts deoxycytidine across the lesion strand during translesion DNA synthesis (TLS). Meanwhile, REV1 has been reported to be involved in homologous recombination (HR) repair. Here we further explore the roles of REV1-interacting proteins RAD51 and RAD51C in REV1-mediated DNA double-strand break (DSB) repair. We found that RAD51 but not RAD51C regulates REV1 recruitment to DSB sites via pulsed laser microirradiation. Interestingly, immunofluorescence staining exhibits that REV1 also regulates RAD51 focus formation in response to CPT treatment. These results suggest that REV1 and RAD51 might be mutually dependent on each other in the REV1-related HR pathway.

Key words: REV1, DNA double-strand breaks repair, homologous recombination, RAD51, RAD51C

Min Huang,Yeran Yang,Xiaoyan Sun,Ting Zhang,Caixia Guo. RAD51 regulates REV1 recruitment to DNA double-strand
break sites[J]. Hereditas(Beijing), 2018, 40(11): 1007-1014.

References

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RAD51 regulates REV1 recruitment to DNA double-strand
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