遗传 ›› 2006, Vol. 28 ›› Issue (12): 1619-1619~1626.doi: 10.1360/yc-006-1619

• 专论与综述 • 上一篇    下一篇

根癌农杆菌介导的香蕉遗传转化研究进展

赵 静, 金志强, 徐碧玉   

  1. (中国热带农业科学院生物技术研究所热带作物生物技术国家重点实验室, 海口 571101)

  • 收稿日期:2006-02-12 修回日期:2006-07-03 出版日期:2006-12-10 发布日期:2006-12-10
  • 通讯作者: 徐碧玉

Progress on Agrobacterium-mediated Transformation of Banana

ZHAO Jing, JIN Zhi-Qiang, XU Bi-Yu   

  1. (State Key Laboratory of Tropical Crops Biotechnology, Institute of Tropical Bioscience and Biotechnology,
    Chinese Academy of Tropical Agricultural Sciences, Haikou
    571101, China)
  • Received:2006-02-12 Revised:2006-07-03 Online:2006-12-10 Published:2006-12-10
  • Contact: XU Bi-Yu

摘要:

香蕉的栽培品种绝大多数是三倍体, 常规育种难度大, 工作周期长, 难以采用传统的育种方法进行遗传改良, 因此转基因技术成为改良香蕉种质的有效方法。根癌农杆菌介导的香蕉的遗传转化从20世纪90年代起取得的了较大的进步, 但仍然存在着很多问题。文章分析了影响农杆菌介导香蕉转化的几个重要环节, 并对其研究现状、存在的问题及应用前景作简要概述。

关键词: 遗传转化, 根癌农杆菌, 香蕉

Abstract:

The development of improved Musa by conventional breeding remains to be a difficult endeavor because of the long generation times, various levels of ploidy, sterility of most edible cultivars and limited genetic variability. Therefore, genetic engineering may offer an alternative method for crop enhancement. The transformation system mediated by Agrobacterium tumefaciens has been used successfully to transform banana during the past 10 years. This article reviews recent progresses on transformation of banana mediated by Agrobacterium tumefaciens and summarizes the factors affecting transformation efficiency. Existing problems and its prospects are briefly discussed.

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