植物转基因整合座位的结构

doi:10.1360/yc-007-0157

遗传 ›› 2007, Vol. 29 ›› Issue (2): 157-157―162.doi: 10.1360/yc-007-0157

植物转基因整合座位的结构

陈建民¹, 姚泉洪², 熊爱生², 马鸿翔³, 洪义欢¹

1. 扬州大学生物科学与技术学院, 扬州 225009;
2. 上海市农业科学院生物技术研究中心, 上海 201106;
3. 江苏省农业科学院遗传生理研究所, 南京 210014

收稿日期:2006-05-10 修回日期:2006-07-11 出版日期:2007-02-10 发布日期:2007-02-10
通讯作者: 陈建民

Structures of transgenic integration loci in transformed plants

CHEN Jian-Min¹, YAO Quan-Hong², XIONG Ai-Sheng², MA Hong-Xiang³
HONG Yi-Huan¹

1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; 2. Agro-Biotechnology Research Center of Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 3. Institute of Genetics and Physiology Jiangsu Academy of Agricultural Science, Nanjing 210014, China

Received:2006-05-10 Revised:2006-07-11 Online:2007-02-10 Published:2007-02-10
Contact: Chen Jianmin

摘要/Abstract

摘要：

通过农杆菌和直接DNA转移技术所获得的转基因植株都具有复杂的转基因座位, 转基因整合染色体和染色体区段是随机的, 但组织培养的选择作用表现为非随机性, 偏向整合于染色体的基因富集区。转基因座位除少数含有完整的单拷贝转基因之外, 大多数转基因座位中外源转基因片段、基因组片段和填充DNA相间而存在。转基因座位中转基因及基因组DNA片段产生缺失、重复和染色体的重排, 转基因的完整性对转基因表达具有重要作用。

关键词: 农杆菌介导, 直接DNA转化, 转基因座位, 染色体重排, 旁侧序列

Abstract:

The plants transformed by either Agrobacterium-mediated or directly delivered DNA transformation possess complex transgenic loci. The transgenes tend to be integrated into gene-rich regions on chromosomes due to selection of transgenic cells during tissue culture rather than integrated elsewhere randomly. Fragments of transferred DNA are interspersed with genomic DNA and filler DNA at most of transgenic loci except a few which contain complete single-copy of transgene. Analysis of transgenic loci flanking sequences indicated that deletion, duplication and chromosomal rearrangement often occurred for transgene and genomic DNA. The complete transgenic structure is important for its expression in transformed plants.

陈建民，姚泉洪，熊爱生，马鸿翔，洪义欢. 植物转基因整合座位的结构[J]. 遗传, 2007, 29(2): 157-157―162.

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植物转基因整合座位的结构

Structures of transgenic integration loci in transformed plants

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