PlcR在炭疽芽胞杆菌A16R中的功能研究

doi:10.16288/j.yczz.15-040

遗传 ›› 2015, Vol. 37 ›› Issue (5): 494-498.doi: 10.16288/j.yczz.15-040

PlcR在炭疽芽胞杆菌A16R中的功能研究

贾晓琳^1,2,王东澍²,高志奇²,冯尔玲²,郑继平^1,3,王恒樑²,郭桂英¹,刘先凯²

1. 海南大学热带生物资源教育部重点实验室,海口 570228;
2. 军事医学科学院生物工程研究所,北京 100071;
3. 海南省热带生物资源可持续利用重点实验室,省部共建国家重点实验培育基地;海口 570228

收稿日期:2015-01-19 出版日期:2015-05-20 发布日期:2015-05-20
通讯作者: 郭桂英,硕士,实验师,研究方向：微生物学。E-mail: 815827434@qq.com;刘先凯,博士,副研究员,研究方向：病原微生物功能基因组学。E-mail: liuxk007@163.com
作者简介:贾晓琳,硕士研究生,专业方向：微生物遗传学。E-mail: leibaihe.2008@163.com;王东澍,博士研究生,专业方向：微生物学。E-mail: wangdongshu@foxmail.com;贾晓琳和王东澍为并列第一作者。
基金资助:
海南省自然科学基金项目(编号：313043)和海南大学青年基金项目(编号：qnjj1229)资助

The function of PlcR in Bacillus anthracis vaccine strain A16R

Xiaolin Jia^{1, 2}, Dongshu Wang², Zhiqi Gao², Erling Feng², Jiping Zheng^{1, 3}, Hengliang Wang², Guiying Guo¹, Xiankai Liu²

1. Ministry of Education Key Laboratory for Tropical Biological Resources, Hainan University, Haikou 570228,China;
2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China;
3. Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-resources-State Key Laboratory Incubation Base, Haikou 570228, China

Received:2015-01-19 Online:2015-05-20 Published:2015-05-20

摘要/Abstract

摘要： 炭疽芽胞杆菌(Bacillus anthracis)、蜡样芽胞杆菌(B. cereus)和苏云金芽胞杆菌(B. thuringiensis)均属于蜡样芽胞杆菌群,在遗传学上有很高的相似性。PlcR (Phospholipase C regulator)在蜡样芽胞杆菌中是十分重要的调控因子,但plcR基因在炭疽芽胞杆菌中发生一个无义突变导致在炭疽芽胞杆菌中产生一个截短PlcR蛋白。为了研究plcR基因对炭疽芽胞杆菌功能的影响,文章以蜡样芽胞杆菌CMCC6330基因组为模板,构建重组表达质粒pBE2A-plcR后导入炭疽芽胞杆菌疫苗株A16R中获得重组菌株,对其进行表型分析。结果显示,炭疽芽胞杆菌重组菌株的溶血活性基本没有恢复,但恢复了部分神经鞘磷脂酶活性,表明将蜡样芽胞杆菌的plcR基因导入炭疽芽胞杆菌后,可以直接激活神经鞘磷脂酶活性。

关键词: 蜡样芽胞杆菌, plcR, 炭疽芽胞杆菌, 神经鞘磷脂酶, 溶血素

Abstract: Bacillus anthracis, B. thuringiensis and B. cereus are members of the B. cereus group. They share high genetic similarity. Whereas plcR (Phospholipase C regulator) usually encodes a functional pleiotropic activator protein in B. cereus and B. thuringiensis isolates, a characteristic nonsense mutation is found in all B. anthracis strains investigated, making the gene dysfunctional. To study the function of PlcR in B. anthracis, we used the B. cereus CMCC63301 genome as a template and constructed a recombinant expression plasmid pBE2A-plcR, and introduced it into the B. anthracis vaccine strain A16R, and then analyzed the activity of the hemolysin and sphingomyelinase. The results showed that transformation of B. anthracis with plasmid pBE2A-plcR carrying the native B. cereus plcR gene active the expression of sphingomyelinase gene, but did not activate expression of hemolysin genes of B. anthracis A16R.

Key words: Bacillus cereus, plcR, Bacillus anthracis, sphingomyelinase, hemolysin

贾晓琳,王东澍,高志奇,冯尔玲,郑继平,王恒樑,郭桂英,刘先凯. PlcR在炭疽芽胞杆菌A16R中的功能研究[J]. 遗传, 2015, 37(5): 494-498.

Xiaolin Jia, Dongshu Wang, Zhiqi Gao, Erling Feng, Jiping Zheng, Hengliang Wang, Guiying Guo, Xiankai Liu. The function of PlcR in Bacillus anthracis vaccine strain A16R[J]. HEREDITAS(Beijing), 2015, 37(5): 494-498.

参考文献

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PlcR在炭疽芽胞杆菌A16R中的功能研究

The function of PlcR in Bacillus anthracis vaccine strain A16R

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