PEX基因在过氧化物酶体形成及真菌致病性中的作用

doi:10.16288/j.yczz.17-079

遗传 ›› 2017, Vol. 39 ›› Issue (10): 908-917.doi: 10.16288/j.yczz.17-079

PEX基因在过氧化物酶体形成及真菌致病性中的作用

高飞雁^1,²(),李玲^2,³,王教瑜²(),王艳丽²,孙国昌²()

1. 浙江师范大学化学与生命科学学院,金华 321004
2. 浙江省农业科学院植物保护与微生物研究所,省部共建国家重点实验室培育基地浙江省植物有害生物防控重点实验室,杭州 310021
3. 浙江农林大学农业与食品科学学院,临安 311300

收稿日期:2017-03-06 修回日期:2017-04-11 出版日期:2017-10-20 发布日期:2017-06-19
作者简介:高飞雁,硕士研究生,专业方向：生物化学。E-mail: 1353423503@qq.com|王教瑜，博士，副研究员，研究方向：真菌功能基因组学。E-mail: wangjiaoyu78@sina.com|孙国昌，博士，研究员，研究方向：植物病原真菌学。E-mail: sungc01@sina.com
基金资助:
国家自然科学基金(31470249);中国博士后基金(2016M592018)

The functions of PEX genes in peroxisome biogenesis and pathogenicity in phytopathogenic fungi

Gao Feiyan^1,²(),Li Ling^2,³,Wang Jiaoyu²(),Wang Yanli²,Sun Guochang²()

1. School of Chemistry and Life Science Zhejiang Normal University, Jinhua 321004, China
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. School of Agricultural and Food Sciences, Zhejiang Agriculture and Forest University, Lin’an 311300, China;

Received:2017-03-06 Revised:2017-04-11 Online:2017-10-20 Published:2017-06-19
Supported by:
the Natural Science Foundation of China(31470249);the China Postdoctoral Science Foundation(2016M592018)

摘要/Abstract

摘要：

过氧化物酶体(peroxisomes)是一类真核生物中普遍存在的细胞器,参与β-氧化、乙醛酸循环等多种重要的生化代谢。研究表明,过氧化物酶体在植物病原真菌侵染寄主过程中具有着举足轻重的作用。参与过氧化物酶体形成与增殖的基因,通常称为PEX基因。近年来,越来越多的PEX基因在植物病原真菌中得到鉴定,真菌过氧化物酶体的形成机制及其在植物病原真菌生长发育和致病过程中的作用越来越受到研究者的关注。本文围绕PEX 基因在过氧化物酶体形成中的作用、对过氧化物酶体相关生化代谢的影响,以及与植物病原真菌生长发育和致病性的关系进行了综述,以期为植物病原真菌致病机理研究和病害防控提供借鉴和参考。

关键词: 过氧化物酶体, PEX基因, 植物病原真菌, 致病性

Abstract:

Peroxisomes are cellular organelles present ubiquitously in eukaryotic cells and are involved in β-oxidation, glyoxylate cycle and a variety of biochemical metabolisms. Recently peroxisomes have been demonstrated to play vital roles in the host infection processes by plant fungal pathogens. The biogenesis of peroxisomes requires a category of proteins named peroxins, which are encoded by the PEX genes. So far, more than 10 PEX genes were isolated in phytopathogenic fungi, and significant research efforts are focused on the mechanism of peroxisome formation and the roles of peroxisome in the development and pathogenicity of fungal pathogens. In this review, we summarize the latest advances in peroxisome biogenesis and functions in pathogenic fungi, including the roles of PEXs in life cycle of peroxisome, peroxisome related metabolisms, and fungal development, infection and pathogenicity, in order to provide references for future studies in plant pathogenic fungi and the control of disease.

Key words: peroxisomes, PEX genes, phytopathogenic fungi, pathogenicity

高飞雁, 李玲, 王教瑜, 王艳丽, 孙国昌. PEX基因在过氧化物酶体形成及真菌致病性中的作用[J]. 遗传, 2017, 39(10): 908-917.

Gao Feiyan, Li Ling, Wang Jiaoyu, Wang Yanli, Sun Guochang. The functions of PEX genes in peroxisome biogenesis and pathogenicity in phytopathogenic fungi[J]. Hereditas(Beijing), 2017, 39(10): 908-917.

参考文献

[1]	Veenhuis M, Salomons FA, Van Der Klei IJ. Peroxisome biogenesis and degradation in yeast: a structure/ function analysis. Microsc Res Tech, 2000, 51(6): 584-600.
[2]	Wanders RJA, Waterham HR. Peroxisomal disorders: the single peroxisomal enzyme deficiencies. Biochim Biophys Acta, 2006, 1763(12): 1707-1720.
[3]	Li L, Wang JY, Zhang Z, Wang YL, Liu MX, Jiang H, Chai RY, Mao XQ, Qiu HP, Liu FQ, Sun GH. MoPex19, which is essential for maintenance of peroxisomal structure and Woronin bodies, is required for metabolism and development in the rice blast fungus. PLoS One, 2014, 9(1): e85252.
[4]	Hu JP. Molecular basis of peroxisome division and proliferation in plants. Int Rev Cell Mol Biol, 2010, 279: 79-99.
[5]	Muench DG, Mullen RT. Peroxisome dynamics in plant cells: a role for the cytoskeleton. Plant Sci, 2003, 164(3): 307-315.
[6]	Kiel JAKW, Veenhuis M, van der Klei IJ. PEX genes in fungal genomes: common, rare or redundant. Traffic, 2006, 7(10): 1291-1303.
[7]	Holroyd C, Erdmann R. Protein translocation machineries of peroxisomes. FEBS Lett, 2001, 501(1): 6-10.
[8]	Kubo Y, Fujihara N, Harata K, Neumann U, Robin GP, O'connell R. Colletotrichum orbiculare FAM1 encodes a novel woronin body-associated Pex22 peroxin required for appressorium-mediated plant infection. mBio, 2015, 6(5): e01305-15.
[9]	Wang JY, Li L, Zhang Z, Qiu HP, Li DM, Fang Y, Jiang H, Chai R, Mao XQ, Wang YL, Sun GC. One of three Pex11 family members is required for peroxisomal proliferation and full virulence of the rice blast fungus Magnaporthe oryzae. PLoS One, 2015, 10(7): e0134249.
[10]	Hynes MJ, Murray SL, Khew GS, Davis MA. Genetic analysis of the role of peroxisomes in the utilization of acetate and fatty acids in Aspergillus nidulans. Genetics, 2008, 178(3): 1355-1369.
[11]	Managadze D, Würtz C, Wiese S, Schneider M, Girzalsky W, Meyer HE, Erdmann R, Warscheid B, Rottensteiner H. Identification of PEX33, a novel component of the peroxisomal docking complex in the filamentous fungus Neurospora crassa. Eur J Cell Biol, 2010, 89(12): 955-964.
[12]	Smith JJ, Aitchison JD. Peroxisomes take shape. Nat Rev Mol Cell Biol, 2013, 14(12): 803-817.
[13]	Klionsky DJ, Cregg JM, Dunn WA, Emr SD, Sakai Y, Sandoval IV, Sibirny A, Subramani S, Thumm M, Veenhuis M, Ohsumi Y. A unified nomenclature for yeast autophagy-related genes. Dev Cell, 2003, 5(4): 539-545.
[14]	Titorenko VI, Terlecky SR. Peroxisome metabolism and cellular aging. Traffic, 2011, 12(3): 252-259.
[15]	Liu MX, Wang JY, Sun GC. Key genes in peroxisome biogenesis and degradation in Magnaporthe oryzae. Chin J Biochem Mol Biol, 2014, 30(6): 554-564.
[15]	刘毛欣, 王教瑜, 孙国昌. 稻瘟病菌过氧化物酶体产生与降解的关键基因. 中国生物化学与分子生物学报, 2014, 30(6): 554-564.
[16]	Purdue PE, Lazarow PB. Pex18p is constitutively degraded during peroxisome biogenesis. J Biol Chem, 2001, 276(50): 47684-47689.
[17]	Hettema EH, Erdmann R, van der Klei I, Veenhuis M. Evolving models for peroxisome biogenesis. Curr Opin Cell Biol, 2014, 29: 25-30.
[18]	Goh J, Jeon J, Kim KS, Park J, Park SY, Lee YH. The PEX7-mediated peroxisomal import system is required for fungal development and pathogenicity in Magnaporthe oryzae. PLoS One, 2011, 6(12): e28220.
[19]	Wang JY, Zhen Z, Wang YL, Li L, Chai RY, Mao XQ, Jiang H, Qiu HP, Du XF, Lin FC, Sun GH. PTS1 peroxisomal import pathway plays shared and distinct roles to PTS2 pathway in development and pathogenicity of. Magnaporthe oryzae. PLoS One, 2013, 8(2): e55554.
[20]	Kiel JAKW, van der Klei IJ. Proteins involved in microbody biogenesis and degradation in Aspergillus nidulans. Fungal Genet Biol, 2009, 46(1 Suppl.): S62-S71.
[21]	Li L, Wang JY, Chen HL, Chai RY, Zhang Z, Mao XQ, Qiu HP, Jiang H, Wang YL, Sun GC. Pex14/17, a filamentous fungus-specific peroxin, is required for the import of peroxisomal matrix proteins and full virulence of Magnaporthe oryzae. Mol Plant Pathol, 2016, doi: 10.1111/mpp.12487.
[22]	Su H, Zhao Y, Yang P, Huang CX, Zhang KQ, Yang JK. Charastics of fungal peroxins. Microbiol China, 2014, 41(4): 725-733.
[22]	苏浩, 赵勇, 杨攀, 黄成翔, 张克勤, 杨金奎. 真菌peroxins蛋白的性质和功能. 微生物学通报, 2014, 41(4): 725-733.
[23]	Opaliński Ł, Bartoszewska M, Fekken S, Liu HY, de Boer R, van der Klei IJ, Veenhuis M, Kiel JAKW. De novo peroxisome biogenesis in Penicillium chrysogenum is not dependent on the Pex11 family members or Pex16. PLoS One, 2012, 7(4): e35490.
[24]	Li XL, Baumgart E, Dong GX, Morrell JC, Jimenez- Sanchez G, Valle D, Smith KD, Gould SJ. PEX11α is required for peroxisome proliferation in response to 4-phenylbutyrate but is dispensable for peroxisome proliferator-activated receptor alpha-mediated peroxisome proliferation. Mol Cell Biol, 2002, 22(23): 8226-8240.
[25]	Liu C, Wu B, Chen W, Li L, Li L, Han P, Zheng CQ, Liu GL. Expression of peroxisome proliferator-activated receptor-γ coactivator-1 in the liver of diabetic animals: experiment with mice. Natl Med J China, 2008, 88(26): 1863-1865.
[25]	刘聪, 吴波, 陈威, 李莉, 李玲, 韩平, 郑长青, 刘国良. 糖尿病大鼠肝脏过氧化物增殖体受体γ辅激活子-1的表达及其意义. 中华医学杂志, 2008, 88(26): 1863-1865.
[26]	Kobayashi S, Tanaka A, Fujiki Y. Fis1, DLP1, and Pex11p coordinately regulate peroxisome morphogenesis. Exp Cell Res, 2007, 313(8): 1675-1686.
[27]	Thoms S, Erdmann R. Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation. FEBS J, 2005, 272(20): 5169-5181.
[28]	Vizeacoumar FJ, Torres-Guzman JC, Tam YYC, Aitchison JD, Rachubinski RA. YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae. J Cell Biol, 2003, 161(2): 321-332.
[29]	Vizeacoumar FJ, Torres-Guzman JC, Bouard D, Aitchison JD, Rachubinski RA. Pex30p, Pex31p, and Pex32p form a family of peroxisomal integral membrane proteins regulating peroxisome size and number in. Saccharomyces cerevisiae. Mol Biol Cell, 2004, 15(2): 665-677.
[30]	Urban M, Bhargava T, Hamer JE. An ATP-driven efflux pump is a novel pathogenicity factor in rice blast disease. EMBO J, 1999, 18(3): 512-521.
[31]	Oku M, Sakai Y. Pexophagy in yeasts. Biochim Biophys Acta, 2016, 1863(5): 992-998.
[32]	Komduur JA, Veenhuis M, Kiel JAKW. The Hansenula polymorpha PDD7 gene is essential for macropexophagy and microautophagy. FEMS Yeast Res, 2003, 3(1): 27-34.
[33]	Farré JC, Subramani S. Peroxisome turnover by micropexophagy: an autophagy-related process. Trends Cell Biol, 2004, 14(9): 515-523.
[34]	Meijer WH, van der Klei IJ, Veenhuis M, Kiel JAKW. ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes. Autophagy, 2007, 3(2): 106-116.
[35]	Soundararajan S, Jedd G, Li XL, Ramos-Pamploña M, Chua NH, Naqvi NI. Woronin body function in Magnaporthe grisea is essential for efficient pathogenesis and for survival during nitrogen starvation stress. Plant Cell, 2004, 16(6): 1564-1574.
[36]	Farré JC, Manjithaya R, Mathewson RD, Subramani S. PpAtg30 tags peroxisomes for turnover by selective autophagy. Dev Cell, 2008, 14(3): 365-376.
[37]	Asakura M, Ninomiya S, Sugimoto M, Oku M, Yamashita SI, Okuno T, Sakai Y, Takano Y. Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus. Colletotrichum orbiculare. Plant Cell, 2009, 21(4): 1291-1304.
[38]	Nazarko TY, Polupanov AS, Manjithaya RR, Subramani S, Sibirny AA. The requirement of sterol glucoside for pexophagy in yeast is dependent on the species and nature of peroxisome inducers. Mol Biol Cell, 2007, 18(1): 106-118.
[39]	Deng YZ, Qu ZW, Naqvi NI. The role of Snx41-based pexophagy in Magnaporthe development. PLoS One, 2013, 8(11): e79128.
[40]	Langfelder K, Streibel M, Jahn B, Haase G, Brakhage AA. Biosynthesis of fungal melanins and their importance for human pathogenic fungi. Fungal Genet Biol, 2003, 38(2): 143-158.
[41]	Patkar RN, Ramos-Pamploña M, Gupta AP, Fan Y, Naqvi NI. Mitochondrial β-oxidation regulates organellar integrity and is necessary for conidial germination and invasive growth in Magnaporthe oryzae. Mol Microbiol, 2012, 86(6): 1345-1363.
[42]	Swiegers JH, Dippenaar N, Pretorius IS, Bauer FF. Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: three carnitine acetyltransferases are essential in a carnitine-dependent strain. Yeast, 2001, 18(7): 585-595.
[43]	Bhambra GK, Wang ZY, Soanes DM, Wakley GE, Talbot NJ. Peroxisomal carnitine acetyl transferase is required for elaboration of penetration hyphae during plant infection by Magnaporthe grisea. Mol Microbiol, 2006, 61(1): 46-60.
[44]	Wang ZY, Soanes DM, Kershaw MJ, Talbot NJ. Functional analysis of lipid metabolism in Magnaporthe grisea reveals a requirement for peroxisomal fatty acid β-oxidation during appressorium-mediated plant infection. Mol Plant Microbe Interact, 2007, 20(5): 475-491.
[45]	Hamilton AJ, Gomez BL. Melanins in fungal pathogens. J Med Microbiol, 2002, 51(3): 189-191.
[46]	Kogej T, Wheeler MH, Rižner TL, Gunde-Cimerman N. Evidence for 1,8-dihydroxynaphthalene melanin in three halophilic black yeasts grown under saline and non-saline conditions. FEMS Microbiol Lett, 2004, 232(2): 203-209.
[47]	Bowman SM, Free SJ. The structure and synthesis of the fungal cell wall. BioEssays, 2006, 28(8): 799-808.
[48]	Ramos-Pamplona M, Naqvi NI. Host invasion during rice-blast disease requires carnitine-dependent transport of peroxisomal acetyl-CoA. Mol Microbiol, 2006, 61(1): 61-75.
[49]	Liu FF, Ng SK, Lu YF, Low W, Lai J, Jedd G. Making two organelles from one: woronin body biogenesis by peroxisomal protein sorting. J Cell Biol, 2008, 180(2): 325-339.
[50]	Jedd G, Chua NH. A new self-assembled peroxisomal vesicle required for efficient resealing of the plasma membrane. Nat Cell Biol, 2000, 2(4): 226-231.
[51]	Kimura A, Takano Y, Furusawa I, Okuno T. Peroxisomal metabolic function is required for appressorium-mediated plant infection by. Colletotrichum lagenarium. Plant Cell, 2001, 13(8): 1945-1957.
[52]	Deng SZ, Gu ZK, Yang N, Li L, Yue XF, Que YW, Sun GC, Wang ZY, Wang JY. Identification and characterization of the peroxin 1 gene MoPEX1 required for infection-related morphogenesis and pathogenicity in Magnaporthe oryzae. Sci Rep, 2016, 6: 36292.
[53]	Fujihara N, Sakaguchi A, Tanaka S, Fujii S, Tsuji G, Shiraishi T, O'connell R, Kubo Y. Peroxisome biogenesis factor PEX13 is required for appressorium-mediated plant infection by the anthracnose fungus Colletotrichum orbiculare. Mol Plant Microbe Interact, 2010, 23(4): 436-445.
[54]	Min K, Son H, Lee J, Choi GJ, Kim JC, Lee YW. Peroxisome function is required for virulence and survival of Fusarium graminearum. Mol Plant-Microbe Interact, 2012, 25(12): 1617-1627.
[55]	Imazaki A, Tanaka A, Harimoto Y, Yamamoto M, Akimitsu K, Park P, Tsuge T. Contribution of peroxisomes to secondary metabolism and pathogenicity in the fungal plant pathogen Alternaria alternata. Eukaryot Cell, 2010, 9(5): 682-694.
[56]	Zhong KL, Li X, Le XY, Kong XY, Zhang HF, Zheng XB, Wang P, Zhang ZG. MoDnm1 dynamin mediating peroxisomal and mitochondrial fission in complex with MoFis1 and MoMdv1 is important for development of functional appressorium in Magnaporthe oryzae. PLoS Path, 2016, 12(8): e1005823.
[57]	Chen XL, Shen M, Yang J, Xing YF, Chen D, Li ZG, Zhao WS, Zhang Y. Peroxisomal fission is induced during appressorium formation and is required for full virulence of the rice blast fungus. Mol Plant Pathol, 2017, 18(2): 222-237.
[58]	Klose J, Kronstad JW. The multifunctional β-Oxidation enzyme is required for full symptom development by the biotrophic maize pathogen Ustilago maydis. Eukaryot Cell, 2006, 5(12): 2047-2061.
[59]	Idnurm A, Howlett BJ. Isocitrate lyase is essential for pathogenicity of the fungus Leptosphaeria maculans to canola(Brassica napus). Eukaryot Cell, 2002, 1(5): 719-724.
[60]	Asakura M, Okuno T, Takano Y. Multiple contributions of peroxisomal metabolic function to fungal pathogenicity in Colletotrichum lagenarium. Appl Environ Microbiol, 2006, 72(9): 6345-6354.
[61]	Solomon PS, Lee RC, Wilson TJG, Oliver RP. Pathogenicity of Stagonospora nodorum requires malate synthase. Mol Microbiol, 2004, 53(4): 1065-1073.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

PEX基因在过氧化物酶体形成及真菌致病性中的作用

The functions of PEX genes in peroxisome biogenesis and pathogenicity in phytopathogenic fungi

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

[1]	李荣, 郭源平, 潘敬新, 郭奕斌. 成骨不全Ⅰ型家系的基因检测和COL1A2基因新突变的致病性鉴定[J]. 遗传, 2015, 37(1): 41-47.
[2]	谢兆辉. 一个抗菌药物新靶点：细菌mRNA降解途径[J]. 遗传, 2013, 35(3): 324-332.
[3]	张昕，姜华，王艳丽，张震，毛雪琴，柴荣耀，邱海萍，杜新法，王教瑜，孙国昌. 真菌类过氧化物酶体增殖因子PEX11基因家族生物信息学研究[J]. 遗传, 2012, 34(5): 635-646.
[4]	苏燕，彭姝彬，李智琼，黄青阳. PPARGC1A基因Thr394Thr/Gly482Ser多态性与2型糖尿病的关联研究[J]. 遗传, 2008, 30(3): 304-308.
[5]	姬森林，黄青阳. PPARγ变异与复杂疾病[J]. 遗传, 2006, 28(8): 993-1001.
[6]	黄俊丽，王贵学. 稻瘟病菌致病性的分子遗传学研究进展[J]. 遗传, 2005, 27(3): 492-498.