遗传 ›› 2017, Vol. 39 ›› Issue (10): 865-876.doi: 10.16288/j.yczz.17-199

• 特邀综述 •    下一篇

酿酒酵母染色体设计与合成研究进展

徐赫鸣1,2(),谢泽雄1,2,刘夺1,2,吴毅1,2,李炳志1,2,元英进1,2()   

  • 收稿日期:2017-05-31 修回日期:2017-09-10 出版日期:2017-09-21 发布日期:2017-09-22
  • 作者简介:徐赫鸣,硕士研究生,专业方向：合成生物学。
  • 基金资助:
    国家自然科学基金(21390203);国家自然科学基金(21576198)

Design and synthesis of yeast chromosomes

Heming Xu1,2(),Zexiong Xie1,2,Duo Liu1,2,Yi Wu1,2,Bingzhi Li1,2,Yingjin Yuan1,2()   

  • Received:2017-05-31 Revised:2017-09-10 Online:2017-09-21 Published:2017-09-22
  • Supported by:
    Supported by the National Natural Science Foundation of China(21390203);Supported by the National Natural Science Foundation of China(21576198)

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摘要：

随着合成生物学的蓬勃发展,基因组学的研究正在由读取基因组信息拓展到以编写基因组信息为主的合成基因组学时代。2009年,由Jef D. Boeke教授提出的人工合成酵母基因组计划(Sc2.0)旨在合成世界上首个真核生物基因组。在美、中、英、法、澳大利亚、新加坡等多国科学家的努力下,目前已经完成1/3的酵母染色体的人工合成。本文从合成基因组学领域的发展历程出发,介绍了Sc2.0计划中酿酒酵母(Saccharomyces cerevisiae)染色体设计与合成的最新进展,包括酿酒酵母9号染色体右臂、3号染色体、2号染色体、5号染色体、6号染色体、10号染色体和12号染色体的设计与合成过程,阐述了其各自的合成策略以及生物学意义,以期为合成基因组学的深入开展提供借鉴与参考。

关键词: 合成基因组学, 合成酵母基因组计划(Sc2.0), 合成型酵母染色体

Abstract:

With the rapid growth and development of synthetic biology, research in the genomics is advancing from genome sequencing to genome synthesis. In 2009, Professor Jef D. Boeke proposed the Synthetic Yeast Genome Project (Sc2.0), which aims to synthesize the world’s first eukaryotic genome. With the efforts of scientists from the United States, China, Britain, France, Australia, Singapore and other countries, a third of the Saccharomyces cerevisiae chromosomes has now been synthesized. In the perspectives of synthetic genomics, we here review the recent progress in the Sc2.0 project, including discussion on the right arm of chromosome Ⅸ, and chromosomes Ⅱ, Ⅴ, Ⅵ, Ⅹ, Ⅻ, in terms of their designs and synthetic strategy as well as the biological significance, thereby providing a reference for further research in synthetic genomics.

Key words: synthetic genomics, synthetic yeast genome project (Sc2.0), synthetic yeast chromosomes