玉米逆境响应相关转录因子ZmC2H2-1基因克隆及功能验证

doi:10.16288/j.yczz.18-119

遗传 ›› 2018, Vol. 40 ›› Issue (9): 767-778.doi: 10.16288/j.yczz.18-119

玉米逆境响应相关转录因子ZmC2H2-1基因克隆及功能验证

汪德州(),莫晓婷,张霞,徐妙云,赵军(),王磊()

中国农业科学院生物技术研究所,北京 100081

收稿日期:2018-05-04 修回日期:2018-06-28 出版日期:2018-09-20 发布日期:2018-09-05
作者简介:汪德州,博士研究生,专业方向：生物化学与分子生物学。E-mail: wangdezhou84@126.com
基金资助:
国家重点研发计划项目(2016YFD0101002);转基因生物新品种培育重大专项(2018ZX0800988B);北京市粮经作物产业创新团队项目(BAIC09-2018)

Isolation and functional characterization of a stress-responsive transcription factor ZmC2H2-1 in Zea mays

Wang Dezhou(),Mo Xiaoting,Zhang Xia,Xu Miaoyun,Zhao Jun(),Wang Lei()

Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China

Received:2018-05-04 Revised:2018-06-28 Online:2018-09-20 Published:2018-09-05
Supported by:
Supported by the National Key Research and Development Program of China(2016YFD0101002);Key project on the Breeding of New Varieties of Genetically Modified(2018ZX0800988B);Program for Economic and Food Crops of Industrial Innovative Research of Beijing(BAIC09-2018)

摘要/Abstract

摘要：

玉米是我国第一大作物,提高玉米的抗逆性是玉米育种的重要目标性状之一。植物C2H2型锌指蛋白广泛参与植物各个时期的生长发育及逆境应答过程。本研究从玉米中分离了转录因子ZmC2H2-1基因并对其功能进行了初步研究。结果表明,ZmC2H2-1属于C2H2锌指蛋白转录因子家族,编码蛋白主要位于细胞核中,酵母自激活实验表明ZmC2H2-1不具有自激活活性;干旱、盐和ABA等逆境可抑制ZmC2H2-1基因在玉米中的表达;过表达ZmC2H2-1基因的拟南芥叶片失水速率更快,在PEG、高盐和ABA处理条件下,与对照相比转ZmC2H2-1基因拟南芥耐逆性降低,以上结果说明ZmC2H2-1基因是作为玉米抗逆的负调控因子参与了逆境胁迫应答。本研究为深入解析玉米ZmC2H2-1的调控网络和玉米的抗逆调控机制奠定了基础。

关键词: 玉米, C2H2型锌指蛋白, 亚细胞定位, 抗逆, 基因调控

Abstract:

Maize has become the most widely planted crops in China and improving maize stress tolerance is one of major target traits for maize breeding. C2H2 zinc finger proteins are widely involved in growth development and stress response in plants. In this study, the transcription factor ZmC2H2-1 gene was isolated from maize and its function was investigated. Our data showed that ZmC2H2-1 belonged to C2H2 transcription factor family, mainly located in the nucleus, and cannot self-activate in yeast. Drought, salt and ABA can inhibit ZmC2H2-1 expression in maize. The water loss rate of excised-leaves was faster in ZmC2H2-1-transgenic Arabidopsis than that in WT. When treated with PEG, high salt and ABA, the stress tolerance was more sensitive in ZmC2H2-1-transgenicplants than WT. These data showed that ZmC2H2-1 played a negative role in stress tolerance in maize. Collectively, this study provides important information for us to analyze ZmC2H2-1 regulatory network and mechanism of stress tolerance in maize.

Key words: maize, C2H2-type zinc finger proteins, subcellular localization, stresses tolerance, gene regulatory

汪德州,莫晓婷,张霞,徐妙云,赵军,王磊. 玉米逆境响应相关转录因子ZmC2H2-1基因克隆及功能验证[J]. 遗传, 2018, 40(9): 767-778.

Wang Dezhou,Mo Xiaoting,Zhang Xia,Xu Miaoyun,Zhao Jun,Wang Lei. Isolation and functional characterization of a stress-responsive transcription factor ZmC2H2-1 in Zea mays[J]. Hereditas(Beijing), 2018, 40(9): 767-778.

参考文献

[1]	Mahajan S, Tuteja N . Cold, salinity and drought stresses: An overview. Arch Biochem Biophys, 2005,444(2):139-158.
[2]	Takatsuji H . Zinc-finger transcription factors in plants. Cell MolLifeSci, 1998,54(6):582-596.
[3]	Zhao LJ, Yi XY, Zeng YL . Research progress on plant stress related C2H2 type zinc finger protein. Mol Plant Breed, 2016,14(3):578-585.
[3]	赵丽娟, 易小娅, 曾幼玲 . 植物逆境相关C2H2型锌指蛋白的研究进展. 分子植物育种, 2016,14(3):578-585.
[4]	Huang J, Wang JF, Zhang HS . Structure and Function of plant C2H2 zinc finger protein. Hereditas(Beijing), 2004,26(3):414-418.
[4]	黄骥, 王建飞, 张红生 . 植物C2H2型锌指蛋白的结构与功能. 遗传, 2004,26(3):414-418.
[5]	Takatsuji H, Mori M, Benfey PN, Ren L, Chua NH . Characterization of a zinc finger DNA-binding protein expressed specifically in Petunia petals and seedlings. EMBO J, 1992,11(1):241-249.
[6]	Tague BW, Gallant P, Goodman HM . Expression analysis of an Arabidopsis C2H2 zinc finger protein gene. Plant Mol Biol, 1996,32(5):785-796.
[7]	Joseph MP, Papdi C, Kozma-Bognár L, Nagy I, López-Carbonell M, Rigó G, Szabados L . The Arabidopsis zinc finger protein3 interferes with abscisic acid and light signaling in seed germination and plant development. Plant Physiol, 2014,165(3):1203-1220.
[8]	Zhou Z, Sun L, Zhao Y, An L, Yan A, Meng X & Gan Y . Zinc Finger Protein 6 (ZFP6) regulates trichome initiation by integrating gibberellin and cytokinin signaling in Arabidopsis thaliana. New Phytol, 2013,198(3):699-708.
[9]	Lippuner V, Cyert MS, Gasser CS . Two classes of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild-type yeast. J Biol Chem, 1996,271(22):12859-12866.
[10]	Kodaira KS, Qin F, Tran LSP, Maruyama K, Kidokoro S, Fujita Y, Yamaguchi-Shinozaki K . Arabidopsis Cys2/His2 zinc-finger proteins AZF1 and AZF2 negatively regulate abscisic acid-repressive and auxin-inducible genes under abiotic stress conditions. Plant Physiol, 2011,157(2):742-756.
[11]	Sakamoto H, Araki T, Meshi T, Iwabuchi M . Expression of a subset of the Arabidopsis Cys2/His2-type zinc-finger protein gene family under water stress. Gene, 2000,248(1-2):23-32.
[12]	Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K . Arabidopsis Cyst/His2-type zinc-finger proteins function as transcription repressors under drought, cold and high-salinity stress conditions. Plant Physiol, 2004,136(1):2734-2746.
[13]	Wang D, Yang J S . Cloning and characterization of cDNA encoding cotton STZ like protein. J Fudan Univ Nat Sci, 2002,41(1):42-46.
[14]	Guo SQ, Huang J, Jiang Y, Zhang HS . Cloning and characterization of RZF71 encoding a C2H2-type zinc figner protein from rice. Hereditas(Beijing), 2007,29(5):607-613.
[14]	郭书巧, 黄骥, 江燕, 张红生 . 水稻C2H2型锌指蛋自基因RZF71的克降与表达分析. 遗传, 2007,29(5):607-613.
[15]	Guo SQ, Zhang HS . Cloning and functional analysis of two zinc finger protein genes, RZF5 and RZF71 from Rice (Oryza Sativa L.)[Dissertation]. Nanjing Agricultural University, 2006, 97-101.
[15]	郭书巧 . 水稻锌指蛋自基因RZF5和RZF71的克降与功能分析[学位论文]. 南京农业大学, 2006, 97-101.
[16]	Kim JC, Lee SH, Cheong YH, Yoo CM, Lee SI, Chun HJ, Yun DJ, Hong JC, Lee SY, Lim CO, Cho MJ . A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. Plant J, 2001,25(3):247-259.
[17]	Sugano S, Kaminaka H, Rybka Z, Catala R, Salinas J, Matsui K, Ohme-Takagi M, Takatsuji H . Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in petunia. Plant J, 2003,36(6):830-841.
[18]	Huang J, Zhang HS, Cao YJ, Wang JF, Yang JS . Cloning and sequencing analysis of a novel C2H2 zinc forger protein cDNA from rice. J Nanjing Agric Univ, 2002,25(2):110-112.
[18]	黄骥, 张红生, 曹雅君, 王东, 王建飞, 杨金水 . 一个新的水稻C2H2型锌指蛋白cDNA的克隆与序列分析. 南京农业大学学报, 2002,25(2):110-112.
[19]	Yuan S, Li X, Li R, Wang L, Zhang C, Chen L, Hao Q, Zhang X, Chen H, Shan Z, Yang Z, Chen S, Qiu D, Ke D, Zhou X . Genome-wide identification and classification of soybean C2H2 zinc finger proteins and their expression analysis in legume-rhizobium symbiosis. Front Microbiol, 2018,9:126.
[20]	Liu XY, Lv SS, Liu R, Fan SX, Liu CJ, Liu RY, Han YY . Transcriptomic analysis reveals the roles of gibberellin- regulated genes and transcription factors in regulating bolting in lettuce (Lactuca sativa L.). PLoS One, 2018,13(2):e0191518.
[21]	Liu Q, Wang Z, Xu X, Zhang H, Li C . Genome-wide analysis of C2H2 Zinc-finger family transcription factors and their responses to abiotic stresses in poplar (Populus trichocarpa). PLoS One, 2015,10(8):e013475.
[22]	Weng H, Yoo CY, Gosney MJ, Hasegawa PM, Mickelbart MV . Poplar GTL1 is a Ca 2+/calmodulin-binding transcription factor that functions in plant water use efficiency and drought tolerance. PLoS One , 2012,7(3):e32925.
[23]	Mun BG, Lee SU, Park EJ, Kim HH, Hussain A, Imran QM, Lee IJ, Yun BW . Analysis of transcription factors among differentially expressed genes induced by drought stress in Populus davidiana. 3 Biotech, 2017,7(3):209.
[24]	Royo J, Gómez E, Barrero C, Mu?iz LM, Sanz Y, Hueros G . Transcriptional activation of the maize endosperm transfercell-specific gene BETL1 by ZmMRP-1 is enhanced by two C2H2 zinc finger-containing proteins. Planta, 2009,230(4):807-818.
[25]	Fedotova AA, Bonchuk AN, Mogila VA, Georgiev PG . C2H2 zinc finger proteins: the largest but poorly explored family of higher eukaryotic transcription factors. Acta Naturae, 2017,9(2):47-58.
[26]	Rogers SO, Bendich AJ . Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985,5(2):69-76.
[27]	Rogers SO, Bendich AJ . Extraction of DNA from plant tissues. Plant Molecular Biology Manual, Springer, Dordrecht, 1989, 73-83.
[28]	Chomczynski P, Sacchi N . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 1987,162(1):156-159.
[29]	Freuler F, Stettler T, Meyerhofer M, Leder L, Mayr LM . Development of a novel Gateway-based vector system for efficient, multiparallel protein expression inEscherichia coli. Protein Expr Purif, 2008,59(2):232-241.
[30]	Arabidopsis Genome Initiative . Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature, 2000,408(6814):796-815.
[31]	Rizhsky L, Davletova S, Liang H, Mittler R . The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis. J Biol Chem, 2004,279(12):11736-11743.
[32]	Song B . Cloning and functional analysis of SCTF-1, STF-2and STF-3 encoding a C2H2-type zinc figner protein from Soybean[Dissertation]. Jilin Agricultural University, 2012, 89-109.
[32]	宋冰. 大豆 C2H2型锌指蛋白基因SCTF-1, STF-2, STF-3的克隆及功能分析[学位论文]. 吉林农业大学, 2012, 89-109.
[33]	Cui LG, Shan JX, Shi M, Gao JP, Lin HX . DCA1 acts as a transcriptional co-activator of DST and contributes to drought and salt tolerance in rice. PLoS Genetics, 2015,11(10):e1005617.
[34]	Cui F, Li J, Ding A, Zhao C, Wang L, Wang X, Li S, Bao Y, Li X, Feng D, Kong L, Wang H . Conditional QTL mapping for plant height with respect to thelength of the spike and internode in two mapping populations of wheat. Theor Appl Genet, 2011,122(8):1517-1536.

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玉米逆境响应相关转录因子ZmC2H2-1基因克隆及功能验证

Isolation and functional characterization of a stress-responsive transcription factor ZmC2H2-1 in Zea mays

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