真核生物基因组长内含子递归剪接事件的分子机制

doi:10.16288/j.yczz.18-182

遗传 ›› 2019, Vol. 41 ›› Issue (2): 89-97.doi: 10.16288/j.yczz.18-182

• 综述 • 下一篇

真核生物基因组长内含子递归剪接事件的分子机制

魏金川¹,徐添翼¹,吴静^1,²(),宋晓峰¹()

^1. 南京航空航天大学自动化学院,南京 211106
^2. 南京医科大学生物医学工程与信息学院,南京 211166

收稿日期:2018-10-30 修回日期:2018-12-24 出版日期:2019-02-20 发布日期:2019-01-03
作者简介:魏金川,硕士研究生,专业方向：生物信息学。E-mail: weijc@nuaa.edu.cn
基金资助:
国家自然科学基金项目资助(61571223)

Molecular mechanisms of recursive splicing events in long introns of eukaryotes

Jinchuan Wei¹,Tianyi Xu¹,Jing Wu^1,²(),Xiaofeng Song¹()

^1. Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China
^2. School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, China

Received:2018-10-30 Revised:2018-12-24 Online:2019-02-20 Published:2019-01-03
Supported by:
[Supported by the National Natural Science Foundation of China](61571223)

摘要/Abstract

摘要：

在高等真核生物基因组转录过程中,一次剪接可完成短内含子的去除,而较长内含子(>10 kb)则需通过多次剪接方可去除。多次剪接去除长内含子的过程通常被称为递归性剪接。已有研究表明,递归性剪接事件与诸多生物学过程及疾病的发生发展有着密切的联系。近年来,关于递归性剪接的研究越来越多,研究者已经在果蝇(Drosophila)和多种脊椎动物基因组转录过程中发现了递归剪接事件,通过不同的生物信息学方法找到了多个递归剪接位点并进行了实验验证。目前国际上对递归性剪接的研究主要集中在递归剪接过程、剪接位点识别及其对生物学过程的影响等方面。本文针对真核生物基因组转录过程中递归剪接事件的分子机制和国内外研究现状进行了综述,旨在为深入理解RNA剪接过程分子机制提供参考。

关键词: 递归剪接, 套索结构, RS位点, Total RNA-seq

Abstract:

Recursive splicing refers to the biological process that long introns are removed in multiple steps during pre-mRNA splicing. In comparison to large introns (>10 kb), most introns in higher eukaryotic genomes are removed in one step during transcription. Previous studies have revealed that recursive splicing events play important roles in many biological processes, including the pathogenesis and development of diseases. In recent years, more researchers have focused on recursive splicing events and found that recursive splicing occurs in Drosophila and many other vertebrates. Multiple recursive splicing sites have been predicted by different bioinformatics methods and verified by experiments. Current researches focus on the process of recursive splicing, recursive splicing site recognition and its influence on biological processes. In this review, we summarize the molecular mechanism of recursive splicing events in eukaryotic genomes and the present development in this field, aiming to lay the basis for further understanding of the mechanisms of RNA splicing.

Key words: recursive splicing, lariat structure, RS-sites, Total RNA-seq

魏金川,徐添翼,吴静,宋晓峰. 真核生物基因组长内含子递归剪接事件的分子机制[J]. 遗传, 2019, 41(2): 89-97.

Jinchuan Wei,Tianyi Xu,Jing Wu,Xiaofeng Song. Molecular mechanisms of recursive splicing events in long introns of eukaryotes[J]. Hereditas(Beijing), 2019, 41(2): 89-97.

参考文献

[1]	Zhang J, Sun X, Qian Y, Maquat LE . Intron function in the nonsense-mediated decay of beta-globin mRNA: indications that pre-mRNA splicing in the nucleus can influence mRNA translation in the cytoplasm. RNA (New York, N.Y.), 1998,4(7):801-815. [DOI]
[2]	Hoffman MM, Birney E . Estimating the neutral rate of nucleotide substitution using introns. Mol Biol Evol, 2007,24(2):522-531. [DOI]
[3]	Chevalier BS, Stoddard BL . Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility. Nucleic Acids Res, 2001,29(18):3757-3774. [DOI]
[4]	Zhang KH . The functions and applications of introns. Chin Anim Husb Vet Med, 2012,39(7):80-83.
[4]	张开慧 . 内含子的功能及应用. 中国畜牧兽医, 2012,39(7):80-83. [DOI]
[5]	Zhao JX, Wang F, Xu ZR, Fan YM . The epigenetic effect on pre-mRNA alternative splicing. Hereditas(Beijing), 2014,36(3):248-255.
[5]	赵金璇, 王芳, 徐峥嵘, 范怡梅 . 表观遗传调控pre- mRNA的选择性剪接. 遗传, 2014,36(3):248-255. [DOI]
[6]	Newman A . RNA splicing. Curr Biol, 1998,8(25):R903. [DOI]
[7]	Yeo G, Burge CB . Maximum entropy modeling of short sequence motifs with applications to RNA splicing signals. J Comput Biol, 2004,11(2-3):377-394. [DOI]
[8]	Li YI, van de Geijn B, Raj A, Knowles DA, Petti AA, Golan D, Gilad Y, Pritchard JK . RNA splicing is a primary link between genetic variation and disease. Science, 2016,352(6285):600-604. [DOI]
[9]	Hang J, Wan R, Yan C, Shi Y . Structural basis of pre-mRNA splicing. Science, 2015,349(6253):1191-1198. [DOI]
[10]	Hatton AR, Subramaniam V, Lopez AJ . Generation of alternative Ultrabithorax isoforms and stepwise removal of a large intron by resplicing at exon-exon junctions. Mol Cell, 1998,2(6):787-796. [DOI]
[11]	Marashi SA, Goodarzi H, Sadeghi M, Eslahchi C, Pezeshk H . Importance of RNA secondary structure information for yeast donor and acceptor splice site predictions by neural networks. Comput Biol Chem, 2006,30(1):50-57. [DOI]
[12]	Rautmann G, Matthes HW, Gait MJ, Breathnach R . Synthetic donor and acceptor splice sites function in an RNA polymerase B (II) transcription unit. Embo J, 1984,3(9):2021-2028. [DOI]
[13]	Burset M, Seledtsov IA, Solovyev VV . Analysis of canonical and non-canonical splice sites in mammalian genomes. Nucleic Acids Res, 2000,28(21):4364-4375. [DOI]
[14]	Schreier P, Aichinger C . Splicing as target for identifying new active substances. 2003. [DOI]
[15]

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

真核生物基因组长内含子递归剪接事件的分子机制

Molecular mechanisms of recursive splicing events in long introns of eukaryotes

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics