遗传 ›› 2020, Vol. 42 ›› Issue (4): 403-421.doi: 10.16288/j.yczz.19-388

• 研究报告 • 上一篇    

小拟南芥MKK基因家族全基因组鉴定及进化和表达分析

李晓翠1, 康凯程1, 黄先忠2,3(), 范永斌1, 宋苗苗1, 黄韵杰1, 丁佳佳1   

  1. 1. 石河子大学生命科学学院,植物基因组学实验室,石河子 832003
    2. 安徽科技学院农学院,凤阳 233100
    3. 石河子大学生命科学学院,石河子 832003
  • 收稿日期:2019-12-30 修回日期:2020-03-15 出版日期:2020-04-20 发布日期:2020-03-27
  • 通讯作者: 黄先忠 E-mail:xianzhongh106@163.com
  • 作者简介:李晓翠,硕士研究生,专业方向:遗传学。E-mail: lxc360720@163.com
  • 基金资助:
    国家自然科学基金项目编号(U1303302);石河子大学国际科技合作项目资助编号(GJHZ201806)

Genome-wide identification, phylogenetic analysis and expression profiling of the MKK gene family in Arabidopsis pumila

Li Xiaocui1, Kang Kaicheng1, Huang Xianzhong2,3(), Fan Yongbin1, Song Miaomiao1, Huang Yunjie1, Ding Jiajia1   

  1. 1. Plant Genomics Laboratory, College of Life Sciences, Shihezi University, Shihezi 832003, China
    2. College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China
    3. College of Life Sciences, Shihezi University, Shihezi 832003, China
  • Received:2019-12-30 Revised:2020-03-15 Online:2020-04-20 Published:2020-03-27
  • Contact: Huang Xianzhong E-mail:xianzhongh106@163.com
  • Supported by:
    Supported by the National Natural Science Foundation of China No(U1303302);International Science and Technology Cooperation Project of Shihezi University No(GJHZ201806)

摘要:

丝裂原活化蛋白激酶激酶(mitogen-activated protein kinase kinase, MAPKK或MKK)是丝裂原活化蛋白激酶(mitogen-activated protein kinase, MAPK)级联的重要组成部分,在植物的生长发育和胁迫应答过程中发挥重要作用。目前,已在多种植物中鉴定了MKK基因家族,但在十字花科植物小拟南芥(Arabidopsis pumila)中MKK基因家族的系统鉴定与分析尚未见报道。为了探索小拟南芥MKK基因家族的进化和功能,本研究通过全基因组分析鉴定了小拟南芥中16个MKK基因,散布于小拟南芥的10条染色体上。基于系统发育分析和多重序列比对,将这些基因分为5个亚族:A亚族(5个)、B亚族(2个)、C亚族(4个)、D亚族(3个)和E亚族(2个)。分子进化和共线性分析表明小拟南芥中存在7对复制基因,分别是ApMKK1-1/1-2ApMKK2-1/2-2ApMKK3-1/3-2ApMKK4-1/4-2ApMKK5-1/5-2ApMKK9-1/9-2ApMKK10-1/10-2,其中ApMKK1-1/1-2在复制事件之后发生了加速进化。结合ApMKKs启动子区的顺式元件分布和ApMKKs在成熟叶片、茎、花和果实以及盐胁迫下的表达模式,结果发现复制基因的表达具有组织特异性和功能多样性。部分复制基因在组织中的表达模式存在差异,但在盐胁迫下的表达模式却基本相同。本研究结果为解析MKK介导的小拟南芥发育过程和非生物胁迫信号转导通路的复杂机制奠定了基础。

关键词: MKK, 小拟南芥, 基因家族, 组织表达, 盐胁迫

Abstract:

Mitogen-activated protein kinase kinase (MAPKK or MKK) is an important component of the MAPK cascade, which plays important roles in plant growth and development as well as in various stress responses. At present, the MKK gene family has been identified in a variety of plants, but there has been no systematic study in Cruciferous plant Arabidopsis pumila. To explore the evolution and function of the MKK gene family in Arabidopsis pumila, 16 ApMKK genes were identified from the Arabidopsis pumila genome by genome-wide analysis, and they were distributed on 10 chromosomes of Arabidopsis pumila. According to phylogenetic analysis and multiple sequence alignment, these putative genes were divided into five known subfamilies, i.e, Groups A, B, C, D, and E, which includes 5, 2, 4, 3, 2 members, respectively. Evolutionary and syntenic analysis showed that there are seven pairs of duplication genes in Arabidopsis pumila: ApMKK1-1/1-2, ApMKK2-1/2-2, ApMKK3-1/3-2, ApMKK4-1/4-2, ApMKK5-1/5-2, ApMKK9-1/9-2, and ApMKK10-1/10-2. Ka/Ks and Tajima analysis indicated that evolution of ApMKK1-1/1-2 was accelerated after the duplication event. Combining the distribution of cis-element in the promoter region of ApMKKs and the expression profile of ApMKKs in mature leaves, stems, flowers and fruits as well as under salt stress, we found that the expressions of paralogous genes (duplication genes) were tissue-specific and their functions were diversified. The expression patterns of some duplicated genes in tissues were different, but the expression patterns under salt stress were basically the same. These results lay the foundation for analyzing the complex mechanisms of MKK-mediated growth and development and abiotic stress signal transduction pathways in Arabidopsis pumila.

Key words: mitogen-activated protein kinase kinase, Arabidopsis pumila, gene family, tissue expression, salt stress