LincRNA AC027700.1在小鼠蜕膜化中的表达研究

doi:10.16288/j.yczz.21-356

遗传 ›› 2022, Vol. 44 ›› Issue (2): 168-177.doi: 10.16288/j.yczz.21-356

LincRNA AC027700.1在小鼠蜕膜化中的表达研究

谭丽萍(), 高茹菲, 尹鑫, 陈雪梅, 李方方, 袁柳, 何俊琳()

重庆医科大学,生殖与发育国际合作联合实验室, 重庆 400016

收稿日期:2021-10-15 修回日期:2022-01-17 出版日期:2022-02-20 发布日期:2022-01-24
通讯作者: 何俊琳 E-mail:2957066450@qq.com;hejunlin@cqmu.edu.cn
作者简介:谭丽萍,在读硕士研究生,专业方向：生殖遗传学。E-mail: 2957066450@qq.com
基金资助:
国家自然科学基金项目编号(31571554);重庆市自然科学基金面上项目编号(cstc2020jcy j-msxmX0041);重庆市研究生导师团队建设项目资助(dstd201809)

The expression of lincRNA AC027700.1 in mouse decidualization

Liping Tan(), Rufei Gao, Xin Yin, Xuemei Chen, Fangfang Li, Liu Yuan, Junlin He()

Joint International Research Laboratory of Reproduction and Development, Chongqing Medical University, Chongqing 400016, China

Received:2021-10-15 Revised:2022-01-17 Online:2022-02-20 Published:2022-01-24
Contact: He Junlin E-mail:2957066450@qq.com;hejunlin@cqmu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China No(31571554);General Project of Chongqing Natural Science Foundation No(cstc2020jcy j-msxmX0041);Postgraduate Tutor Team Construction Project of Chongqing No(dstd201809)

摘要/Abstract

摘要：

长链非编码RNA(long non-coding RNA, lncRNA)是一类长度大于200 nt、不具有蛋白编码潜能的RNA分子。在细胞生长发育、物质代谢以及疾病等的发生发展过程中起关键调控作用,但在蜕膜化相关领域研究报道较少。为了探究lincRNA AC027700.1在早孕小鼠子宫内膜中的表达规律,初步探讨AC027700.1在小鼠蜕膜化中的作用,本研究利用qRT-PCR检测AC027700.1在小鼠孕第6 d胚胎着床点及着床旁子宫组织中的表达;构建了假孕小鼠体内人工诱导蜕膜化模型和原代小鼠子宫内膜基质细胞体外诱导蜕膜化模型,qRT-PCR检测AC027700.1在人工诱导蜕膜化的组织和细胞中的表达;通过分离细胞核、细胞质RNA,qRT-PCR检测AC027700.1在胞核和胞质中的相对表达水平;利用GOseq和KOBAS软件对AC027700.1下游靶基因进行GO和KEGG分析。结果表明,AC027700.1在小鼠孕第6 d着床点子宫组织中的表达显著高于着床旁;AC027700.1在成功诱导蜕膜化的子宫内膜组织及细胞中的表达显著高于未诱导的组织及细胞;AC027700.1主要定位于细胞核内;AC027700.1下游靶基因主要富集于自噬通路、细胞周期以及RNA转运等通路。本研究初步揭示了lincRNA AC027700.1可能与早孕子宫内膜蜕膜化有关,但具体作用及调节机制还有待进一步研究。

关键词: lincRNA, AC027700.1, 蜕膜化, 子宫内膜, 着床

Abstract:

Long non-coding RNAs (lncRNAs), which belong to the non-protein-coding RNAs, are greater than 200 nt in length. Although they have been found to play crucial roles in the regulation of cell growth and development, cell metabolism and the development of diseases, they are rarely reported in decidualization. The objective of our study is to explore the expression of lincRNA AC027700.1 in the endometrium of early pregnant mice and its role in decidualization. The expression of AC027700.1 in uterine tissues at implantation sites and inter implantation sites on the 6th day of pregnancy were detected by qRT-PCR. The relative expression of AC027700.1 in an in vivo model of induced decidualization in pseudopregnant mice and in in vitro model of induced decidualization in primary stromal cells and nucleus/cytoplasmic fractions were detected by qRT-PCR. GO and KEGG analysis of downstream target genes were performed by GOseq and KOBAS, respectively. The results show that AC027700.1 expression is significantly increased in tissues at implantation sites on the 6th day of pregnancy and in decidualized endometrial tissues and stromal cells. Furthermore, AC027700.1 localizes in the nuclear fraction and the downstream targeted genes are mainly involved in autophagy, cell cycle and RNA transport pathways. This study revealed that lincRNA AC027700.1 may be involved in decidualization of endometrium in early pregnancy, but the specific role and regulatory mechanism remain to be further studied.

Key words: lincRNA, AC027700.1, decidualization, endometrium, implantation

谭丽萍, 高茹菲, 尹鑫, 陈雪梅, 李方方, 袁柳, 何俊琳. LincRNA AC027700.1在小鼠蜕膜化中的表达研究[J]. 遗传, 2022, 44(2): 168-177.

Liping Tan, Rufei Gao, Xin Yin, Xuemei Chen, Fangfang Li, Liu Yuan, Junlin He. The expression of lincRNA AC027700.1 in mouse decidualization[J]. Hereditas(Beijing), 2022, 44(2): 168-177.

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基因	序列(5'→ 3')
β-actin	F: GTGCTATGTTGCTCTAGACTTCG
β-actin	R: ATGCCACAGGATTCCATACC
Dtprp	F: TACCCACGTAAGGTCATC
Dtprp	R: CTCAGAGCCAGAAATCA
AC027700.1	F: CGGAAGTTCAGCAGAAGGAAGAGG
AC027700.1	R: GCCCACACCTTATTCACCAAGACC
U3	F: CTGGCTCTAGGTGCTGCTTGATTG
U3	R: ACTGCGTTCCCTCCCTCTCAC
U4	F: GCGCAGTGGCAGTATCGTAGC
U4	R: CAAGTCGTCATGGCGGGGTATTG

LincRNA AC027700.1在小鼠蜕膜化中的表达研究

The expression of lincRNA AC027700.1 in mouse decidualization

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