单细胞转录组测序在少突胶质谱系细胞异质性与神经系统疾病中的应用

doi:10.16288/j.yczz.22-306

遗传 ›› 2023, Vol. 45 ›› Issue (3): 198-211.doi: 10.16288/j.yczz.22-306

单细胞转录组测序在少突胶质谱系细胞异质性与神经系统疾病中的应用

韩熙(), 罗富成()

昆明理工大学灵长类转化医学研究院，省部共建非人灵长类生物医学国家重点实验室，昆明 650500

收稿日期:2022-11-07 修回日期:2023-01-13 出版日期:2023-03-20 发布日期:2023-01-18
通讯作者: 罗富成 E-mail:hxsci@outlook.com;luofc@lpbr.cn
作者简介:韩熙，在读硕士研究生，专业方向：神经生物学。E-mail: hxsci@outlook.com
基金资助:
国家自然科学基金-地区基金(82060234);云南省基础研究计划项目(202101BE070001-065);云南省科技厅科技人才与平台计划(202005AE160019)

Application of single-cell RNA sequencing in probing oligodendroglia heterogeneity and neurological disorders

Xi Han(), Fucheng Luo()

State Key Laboratory of Primate Biomedical Research and Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China

Received:2022-11-07 Revised:2023-01-13 Online:2023-03-20 Published:2023-01-18
Contact: Luo Fucheng E-mail:hxsci@outlook.com;luofc@lpbr.cn
Supported by:
the National Natural Science Foundation of China-Regional Fund(82060234);the Basic Research Projects in Yunnan Province(202101BE070001-065);the Science and Technology Talents and Platform Program of Yunnan Science and Technology Department(202005AE160019)

摘要/Abstract

摘要：

少突胶质细胞是中枢神经系统中形成髓鞘的高度特化的胶质细胞，由少突胶质前体细胞分化而来。长期以来，围绕少突胶质谱系细胞开展的研究主要集中在少突胶质细胞发育、髓鞘形成以及少突胶质谱系细胞在神经系统疾病中的作用等。新兴的单细胞转录组测序技术可以在转录组层面鉴定出特定类型细胞，为少突胶质谱系细胞的研究提供助力。本综述主要关注常见单细胞测序技术的发展以及它们在少突胶质细胞功能异质性和神经系统疾病研究中的应用，并对已取得的成果进行总结阐述，为单细胞测序技术在中枢神经系统疾病中少突胶质谱系细胞相关研究的应用和开发提供思路和参考。

关键词: 少突胶质细胞, 单细胞转录组测序, 细胞异质性, 神经系统疾病

Abstract:

In the central nervous system, oligodendrocytes are highly specialized myelinating glial cells that originate from oligodendrocyte precursor cells. In the past, research centered on the oligodendrocyte development, myelination, and the role of oligodendrocyte lineage in neurological disorders. The emerging single-cell RNA sequencing technology is a new tool to specifically identify cell-types at the transcriptome level, and recently have also been used to investigate oligodendrocyte lineage-related issues. In this review, we summarize the recent developments of single-cell RNA sequencing technologies and their application in the oligodendroglia heterogeneity and neurological disorders, thereby providing new ideas and references for the utilization of single-cell RNA sequencing technology in the research field of oligodendrocyte lineage and neurological disorders.

Key words: oligodendrocytes, single-cell RNA sequencing, heterogeneity, neurological disorders

韩熙, 罗富成. 单细胞转录组测序在少突胶质谱系细胞异质性与神经系统疾病中的应用[J]. 遗传, 2023, 45(3): 198-211.

Xi Han, Fucheng Luo. Application of single-cell RNA sequencing in probing oligodendroglia heterogeneity and neurological disorders[J]. Hereditas(Beijing), 2023, 45(3): 198-211.

图/表 2

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测序平台	推出时间	细胞通量	细胞条码	单分子标签	细胞分离方式	扩增方式	覆盖范围	参考文献
STRT-seq	2011	~100	√	×	cell picking	PCR	5′ end	[4]
Smart-seq	2012	~100	×	×	FACS	PCR	full length	[5]
CEL-seq	2012	~100	√	×	FACS	IVT	3′ end	[7]
Smart-seq2	2013	~100	×	×	FACS	PCR	full length	[6]
Fluidigm C1	2013	~100	×	×	Microfluidic	PCR	full length	[10]
Drop-seq	2015	>10000	√	√	Microdroplets	PCR	3′ end	[12]
inDrop-seq	2015	>10000	√	√	Microdroplets	IVT	3′ end	[22]
CEL-seq2	2016	~100	√	√	Microfluidic	IVT	3′ end	[9]
10× Genomics	2016	>10000	√	√	Microdroplets	PCR	3′ end	[13]
BD Rhapsody	2018	>10000	√	√	Microfluidic	PCR	3′ end	[15]
DNBelab C4	2019	>10000	√	√	Microdroplets	PCR	3′ end	[17]

单细胞转录组测序在少突胶质谱系细胞异质性与神经系统疾病中的应用

Application of single-cell RNA sequencing in probing oligodendroglia heterogeneity and neurological disorders

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[2]	屈亮, 李素, 仇华吉. 单细胞RNA测序技术在病毒研究中的应用[J]. 遗传, 2020, 42(3): 269-277.
[3]	王建, 张凯翔, 芦国珍, 赵湘辉. 5-羟甲基胞嘧啶及其TET氧合酶在神经系统发育和相关疾病中的研究进展[J]. 遗传, 2017, 39(12): 1138-1149.
[4]	王琳淇. 适应性策略：人类致病真菌新生隐球菌的“杀手锏”[J]. 遗传, 2015, 37(5): 436-441.