遗传 ›› 2008, Vol. 30 ›› Issue (3): 277-282.doi: 10.3724/SP.J.1005.2008.00277

• 综述 • 上一篇    下一篇

Min基因突变小鼠模型在肠道肿瘤研究中的应用

盛弘强, 陈俭, 来茂德   

  1. 浙江大学医学院病理学与病理生理学系, 杭州 310058

  • 收稿日期:2007-08-15 修回日期:2007-11-05 出版日期:2008-03-10 发布日期:2008-03-10
  • 通讯作者: 盛弘强

Use of Apc(Min/+) mouse model in the studies of intestinal tumors

SHENG Hong-Qiang, CHEN Jian, LAI Mao-De

  

  1. Department of Pathology and Pathophysiology, School of Medicine, Zhejiang University, Hangzhou 310058, China
  • Received:2007-08-15 Revised:2007-11-05 Online:2008-03-10 Published:2008-03-10
  • Contact: SHENG Hong-Qiang

摘要:

迄今为止, 肠道肿瘤相关的基因突变小鼠或敲除小鼠大约有30多种, Min(multiple intestinal neoplasia)小鼠是具有肠道多发性腺瘤特征的Apc基因突变小鼠, 被认为是当前较为理想的家族性腺瘤性息肉病(FAP)的研究模型。APC基因是Wnt途径中重要的抑癌基因, 该途径不仅是动物胚胎发育过程中关键的信号转导途径, 也对结直肠肿瘤的发生发展起到不同寻常的作用。对Min小鼠模型历史、分子遗传学与表型特征、肠道肿瘤与Wnt途径异常、抑癌基因甲基化、TGF-b途径和多药耐药基因等内容进行了介绍, 并分析了该小鼠模型在抗结直肠肿瘤药物研究中的应用和意义。

关键词: Wnt基因, Min小鼠, APC基因

Abstract:

More than thirty kinds of mutant or knockout mice bearing intestinal neoplasm have been reported up to the present. Apc(Min/+) mouse holding multiple intestinal neoplasia, provides an appropriate model to evaluate human familial adenomatous polyposis. APC is an important tumor-suppressor gene in the Wnt pathway, which is involved in the pivotal signal transduction cascade in animal embryogenesis and colorectal carcinogenesis. Apc(Min/+) mouse model was pre-sented as aspects of the strain background, genotype/phenotype, divergent canonical Wnt signaling pathway, methylation of tumor-suppressor gene, TGF-b signaling pathway and multidrug resistance gene, etc. This review also introduced the appli-cation and signification of the mouse model in studies of anti-colorectal tumor drugs.