遗传 ›› 2011, Vol. 33 ›› Issue (5): 504-511.doi: 10.3724/SP.J.1005.2011.00504

• 综述 • 上一篇    下一篇

外源基因在转基因动物中遗传和表达的稳定性

孔庆然, 刘忠华   

  1. 东北农业大学生命科学学院, 哈尔滨 150030
  • 收稿日期:2010-09-24 修回日期:2010-11-25 出版日期:2011-05-20 发布日期:2011-05-25
  • 通讯作者: 刘忠华 E-mail:liu086@yahoo.com
  • 基金资助:

    转基因生物新品种培育科技重大专项(编号: 2008ZX08006-002, 2009ZX08006-001B)和东北农业大学“创新团队”发展计划资助项目

Inheritance and expression stability of transgene in transgenic animals

KONG Qing-Ran, LIU Zhong-Hua   

  1. College of Life Science, Northeast Agricultural University of China, Harbin 150030, China
  • Received:2010-09-24 Revised:2010-11-25 Online:2011-05-20 Published:2011-05-25
  • Contact: LIU Zhong-Hua E-mail:liu086@yahoo.com

摘要: 转基因技术经过近半个世纪的发展, 已成为当今生物技术研究的热点。近10多年来, 与核移植技术的结合, 转基因效率大大提高, 携带有不同外源基因的不同种类的转基因动物迅速增加。但是, 成功获得转基因动物并不是转基因动物研究的最终目的, 如何利用转基因技术为人类的需求服务才是科研人员始终面对的课题。在畜牧生产领域, 通过转基因技术培育家畜新品种是转基因技术应用的重要体现, 在我国这方面已经引起了广泛关注。但迄今为止, 外源基因在转基因动物中遗传和表达的稳定性仍然是亟待解决的问题, 究其原因, 这主要与位置效应、外源基因的表观遗传学修饰和遗传效率相关, 文章结合目前的研究进展和本实验室的研究结果, 从这3方面阐述其作用机制, 期望为转基因动物遗传育种向产业化的迈进提供一定的理论探讨。

关键词: 转基因动物, 外源基因遗传, 外源基因表达

Abstract: Transgenic technology is one of the most hotspots in biology. In the past decade, the progress in animal cloning has provided an alternative method to improve transgenic efficiency. Many kinds of transgenic animals have been successfully produced via the combination of transfection and nuclear transfer. However, the ultimate aim of transgenesis is not to produce several transgenic animals, but to service for the needs of human. In animal production, transgenic technology has been used to breed new livestock, which has received a lot of attention in China. It has been evidenced that inheritance and expression instability of transgene in transgenic animals is still the major limitation, which is attributed to position effect, epigenetic modification, and hereditary efficiency of transgene. In this review, we discussed the three points for promoting the industrialization of animal transgenic breeding.

Key words: transgenic animals, transgene inheritance, transgene expression