遗传 ›› 1993, Vol. 15 ›› Issue (1): 20-23.

• 论文 • 上一篇    下一篇

从小麦雄性不育系的未授粉子房诱导单倍体植株

张素芳 张召铎 刘植义 沈银柱 司智海Zhang Sufang Zhang Zhaoduo Liu Zhi Shen Yinzhu Si Zhihai   

  1. 河北师范大学生物系,石家庄050016Department of Biology, Hebei Normal University, Shijiazhuang 050016
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1993-02-10 发布日期:1993-02-10

Induction of Haploid Plants from Unpollinated Ovaries of MaleSterile Lines of W heat in vitro

  • Received:1900-01-01 Revised:1900-01-01 Online:1993-02-10 Published:1993-02-10

摘要: 采用N6培养基为基本培养基,附加不同水平的生长素,培养普通小麦、小麦雄性不育系及其保持系共10个材料的未授粉子房,所用的材料都能诱导出愈伤组织,出愈率在4.2-38.1%。经分化培养分别从T型不育系和太谷核不育材料获得了单倍体植株。供试材料的基因型、培养基中的生长素的种类及用量,对诱导愈伤组织及分化绿苗有重要的作用。
The unpollinated ovaries of male sterile lines and its manitainers of wheat, as well as common wheat, were cultured on N6 medium in vitro,when the pollen of the same panicle were at uninucleate stage. Callus were obtained from all of the materials, haploid plantlets were regenerated from the unpollinated ovaries of timopheevi cytoplasm male sterile line (T75-3369A) and Taigu nucleus male sterile line (Tal x C6841).

关键词: Haploid plants, Unpollinated ovaries, 未授粉子房.单倍体植株 Male sterile lines of wheat, 小麦雄性不育系