遗传 ›› 2000, Vol. 22 ›› Issue (6): 392-394.

• 论文 • 上一篇    下一篇

应用微卫星标记鉴别水稻籼粳亚种

樊叶杨;庄杰云;吴建利;孙宝龙;郑康乐 FAN Ye-yang;ZHUANG Jie-yun;WU Jian-li;SUN Bao-long;ZHENG Kang-le   

  1. 中国水稻研究所国家水稻改良中心,杭州 310006 National Center for Rice Improvement,CNRRI,Hangzhou 31006,China
  • 收稿日期:1900-01-01 出版日期:2000-12-10 发布日期:2000-12-10

SSLP-based Identification of Subspecies in Rice (Oryza sativa L.)

  • Received:1900-01-01 Online:2000-12-10 Published:2000-12-10

摘要: 应用70个微卫星标记分析了3个籼稻测验种和3个粳稻测验种的多态性,发现其中36个标记可以区分籼粳测验种。再以18个籼粳品种进一步筛选,找到了分布于12条染色体的21个籼粳特异性微卫星标记。在这21个标记中,20个在籼粳亚种间带型相异,其中7个在亚种内带型一致,13个在亚种内带型不一致;1个标记在12个籼稻品种和1个粳稻品种检测到相同的带型,其余11个粳稻品种具有另一种带型。微卫星标记和RFLP标记检测籼粳亚种不仅具有一致性,而且还有互补性。
Abstract:Six indica and japonica testers were assayed using 70 microsatellite markers.Thirty-six markers distinguishing indicas from japonicas were detected.By further-screening among 18 indica and japonica varieties,21 markers distributed on 12 rice chromosomes were found to be indica-japonica differentiated.No indica varieties shared same patterns with any japonica varieties at 20 marker loci,of which identical patterns were observed within subspecies at 7 loci while within-subspecies variations were observed at 13 loci.At the remaining locus,12 indica and 1 japonica varieties had the same allele,while other 11 japonica varieties had another allele.It also showed that SSLP was not only consistent,but also complementary,to RFLP for the subspecies identification.

关键词: 亚洲栽培稻
Key words,
microsatellite DNA markers, 籼粳特异性, 微卫星DNA标记, indica-japonica differentiation