遗传 ›› 2001, Vol. 23 ›› Issue (2): 128-130.

• 论文 • 上一篇    下一篇

RAPD分析在绢丝昆虫亲缘关系研究中的应用I.蓖麻蚕品种间的遗传差异

左正宏1;桂慕燕2;王学民2;陈元霖2 ZUO Zheng-hong1;GUI Mu-yan2;WANG Xue-min2;CHEN Yuan-lin2   

  1. 1.湖南省岳阳卫生学校,岳阳 414000 2.福建省厦门大学生物学系,厦门 361005 1.Yueyang Heath School of Hunan,Yueyang 414000; 2.Dept.of Bio.Xiamen University,Xiamen 361005,China
  • 收稿日期:1900-01-01 出版日期:2001-04-10 发布日期:2001-04-10

Application of RAPD Technique in Genetic Relationship Study of Silk Insect I. Genetic Variance in Eri Silkworm

  • Received:1900-01-01 Online:2001-04-10 Published:2001-04-10

摘要: 用RAPD技术对蓖麻蚕基因组DNA进行多态性研究,分析了5个蓖麻蚕品种间的遗传差异。结果表明,所采用的40个随机引物中,有27个引物扩增谱带清晰且重复性较好,扩增总片段数达243个,单个引物的扩增片段数在4~17之间,平均为9条,片段大小在0.33~3.0kb之间。不同蓖麻蚕品种间的遗传距离(D)在0.0683~0.1603之间,根据D值,由UPGMA聚类分析软件绘制了它们的聚类分子树。
Abstract:Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among eri sickworm. The genetic variance of five erisickworm was studied. The result showed that: 27 of 40 arbitrary primers could amplify clearly with repeatable bands.243 fragments were obtained.Each primer gave 4~17 bands and the average was 9.The length of the band was 0.33~3.0kb. The genetic distance (D) value between different breeds of Eri Silkworm was 0.0683~0.1603. The D value was used to construct a dendrogram by UPGMA.

关键词: RAPD, 品种差异 Keywords, eri silkworm, RAPD, 蓖麻蚕