用交错延伸剪接PCR技术扩增油菜花粉育性基因MS2Bnap全长cDNA序列

遗传 ›› 2001, Vol. 23 ›› Issue (6): 553-331.

用交错延伸剪接PCR技术扩增油菜花粉育性基因MS2Bnap全长cDNA序列

李德谋;罗小英;侯磊;裴炎;方卫国;杨光伟 LI De-mou;LUO Xiao-ying;HOU Lei;PEI Yan;FANG Wei-guo;YANG Guang-wei

西南农业大学生物技术中心,重庆,北碚,400716 Center for Biotechnology,SUTH west Agricultural University,Chongqing 400716,China

收稿日期:1900-01-01 出版日期:2001-12-10 发布日期:2001-12-10

Amplification of Pollen Fertility Gene MS2Bnap Full-length cDNA from Brassica napus Using SOE by PCR

Received:1900-01-01 Online:2001-12-10 Published:2001-12-10

摘要/Abstract

摘要： 交错延伸剪接PCR是一种用于分子进化研究的DNA改组技术.本实验利用其原理,将已获得的油菜花粉育性基因MS2Bnap有部分序列重叠的三段PCR产物作为模板进行扩增,获得了花粉育性基因MS2Bnap的全长cDNA序列。
Abstract:SOE by PCR is one of DNA shuffling technologies for molecular evolution engineering.Using the theory of SOE by PCR,the full-length cDNA sequence of pollen fertility gene MS2Bnap of Brasscia napus was amplified with three obtained fragments as templates which have part overlapping of MS2Bnap sequence.

关键词: 花粉育性基因MS2Bnap, 交错延伸剪接PCR, 全长cDNA序列
Key words, Pollen fertility gene MS2Bnap, splicing by overlap extension by PCR (SOE by PCR)

李德谋，罗小英，侯磊，裴炎，方卫国，杨光伟. 用交错延伸剪接PCR技术扩增油菜花粉育性基因MS2Bnap全长cDNA序列[J]. 遗传, 2001, 23(6): 553-331.

LI De-mou, LUO Xiao-ying, HOU Lei, PEI Yan, FANG Wei-guo, YANG Guang-wei. Amplification of Pollen Fertility Gene MS2Bnap Full-length cDNA from Brassica napus Using SOE by PCR[J]. HEREDITAS, 2001, 23(6): 553-331.